BBa_K1076001 1 BBa_K1076001 Flanking genes for FadR deletion 2013-09-12T11:00:00Z 2015-05-08T01:09:03Z From genomic sequence Part of the upstream and dowstream regions of the gene FadR, containing part the genes SpoVR and NhaB respectively.This parts have Nde1 complementary site and they were ligate together. This method garantee generation of a deleted copy of the target gene using a two step assymmetric/crossover PCR amplification. The target in this case is the FadR. In other words its a system to knock down the FadR gene which is transcribed into a regulatory intake fatty-acid protein. false false _1385_ 0 13948 9 It's complicated false Design PCR primers to amplify the regions flanking the gene FadR. Subsequent to amplification, these flanking regions are fused together via a complementary ???tag??? region. This fusion product will then be inserted into the suicide vector pNPTS138. false Luna Lacerda, Adolfo Mota, Carlos Gustavo Nunes BBa_K1076001_sequence 1 tacatatgtaacgatcaagataggcgagagcgctattttgtaacacaaagttaaaagccgtctttatttgaagacggctttattatttgcggcatgattaggatgtcgtattgtttggacaagttgctaacacattaatgctggcatcggcactttgttgctcaagggtaacattaaatccccagagtcgatgtagatgcttcaacacttcgtgatagctattggcaagcggaatgtcgttgcttggaacatatcttagggttaatgatctatcgccatttatttcaacatggtgtacttggatattaggttctatgtttgacaagttatattgctgtgaaagtaattgacgaatatcacggtaaccttgttcatcgtgaattgctgatacagaaagataactgcgtttttcatcatcatgaatgccaaataatttaaattggcgaatgacttttggggaaagatattggctaataaaactctcatccttaaaatgctgcatggcaaaatgtaacgtctcaagccaattactgcctgcgatatcaggaaaccatactttatcttcatcggtcggtgcttcacaaattcgcctgatatcggtgaacatggcaaaacctagagcataggggttaataccgctgtaatagcggctgttatactcaggctgcgcaacaacacctgtatggctttgtaaaaactccatcataaaacggtcggtcacgacaccatcatcgtataagtgatttaaaatcgtgtagtgccagaagggatccgatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z