LOCUS BBa_K1077002 3265 bp DNA linear 22-Dec-2024 DEFINITION Inducible fimE and hbiF recombinase generator (tet and lux). ACCESSION BBa_K1077002 VERSION BBa_K1077002.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..688 /label=tetR CDS 62..688 /label=BBa_I732080 stem_loop 708..751 /label=stem_loop misc_feature 697..776 /label=BBa_B0010 terminator 697..776 /label=BBa_B0010 stem_loop 792..811 /label=T7 TE misc_feature 818..818 /label=stop polyA_site 812..825 /label=polya misc_feature 785..825 /label=BBa_B0012 terminator 785..825 /label=BBa_B0012 misc_feature 44..825 /label=BBa_K176001 misc_feature 834..852 /label=TetR 1 misc_feature 834..887 /label=BBa_R0040 promoter 853..858 /label=-35 misc_feature 859..877 /label=TetR 2 promoter 876..881 /label=-10 promoter 834..887 /label=BBa_R0040 misc_feature 900..903 /label=conserved RBS 896..907 /label=BBa_B0034 CDS 914..1471 /label=BBa_K137007 stem_loop 1491..1534 /label=stem_loop misc_feature 1480..1559 /label=BBa_B0010 terminator 1480..1559 /label=BBa_B0010 stem_loop 1575..1594 /label=T7 TE misc_feature 1601..1601 /label=stop polyA_site 1595..1608 /label=polya misc_feature 1568..1608 /label=BBa_B0012 terminator 1568..1608 /label=BBa_B0012 terminator 1480..1608 /label=BBa_B0015 misc_feature 1619..1635 /label=lac O1 promoter 1636..1641 /label=-35 misc_feature 1642..1658 /label=lac O1 promoter 1659..1664 /label=-10 misc_feature 1617..1670 /label=BBa_R0011 promoter 1617..1670 /label=BBa_R0011 misc_feature 1684..1687 /label=conserved RBS 1680..1691 /label=BBa_B0034 misc_feature 1698..1699 /label=prefix misc_feature 1871..1871 /label=T misc_feature 2189..2189 /label=A CDS 1698..2447 /label=luxR misc_feature 1698..2453 /label=BBa_C0062 misc_feature 2454..2478 /label=Help:Barcodes CDS 1698..2453 /label=BBa_C0062 stem_loop 2498..2541 /label=stem_loop misc_feature 2487..2566 /label=BBa_B0010 terminator 2487..2566 /label=BBa_B0010 stem_loop 2582..2601 /label=T7 TE misc_feature 2608..2608 /label=stop polyA_site 2602..2615 /label=polya misc_feature 2575..2615 /label=BBa_B0012 terminator 2575..2615 /label=BBa_B0012 misc_feature 2624..2643 /label=LuxR/HSL promoter 2643..2648 /label=-35 promoter 2665..2670 /label=-10 misc_feature 2676..2676 /label=start misc_feature 2624..2678 /label=BBa_R0062 promoter 2624..2678 /label=BBa_R0062 misc_feature 1617..2678 /label=BBa_F2622 misc_feature 2691..2694 /label=conserved RBS 2687..2698 /label=BBa_B0034 misc_feature 2705..3265 /label=BBa_K880000 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt cctaataata ctagagccag gcatcaaata aaacgaaagg 721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 841 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 901 ggagaaatac tagatgatgc aggcggtttg ttacggggca acgggagcca gagattattg 961 tcttattctg ttggcatatc ggcatgggat gcgtattagt gaactgcttg atctgcatta 1021 tcaggacctt gaccttaatg aaggtagaat aaatattcgc cgactgaaga acggattttc 1081 taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg gaacgctgga cccaggaacg 1141 tgctaactgg aaaggcgctg accggactga cgctatattt atttctcgcc gcgggagtcg 1201 gctttctcgc cagcaggcct atcgcattat tcgcgatgcc ggtattgaag ctggaaccgt 1261 aacgcagact catcctcata tgttaaggca tgcttgcggt tatgaattgg cggagcgtgg 1321 tgcagatact cgtttaattc aggattatct cgggcatcga aatattcgcc atactgtgcg 1381 ttataccgcc agtaatgctg ctcgttttgc cggattatgg gaaagaaata atctcataaa 1441 cgaaaaatta aaaagagaag aggtttaata atactagagc caggcatcaa ataaaacgaa 1501 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1561 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 1621 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 1681 aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga ataattaata 1741 aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa 1801 tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg 1861 atatttcaat cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt 1921 taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga 1981 atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa 2041 catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa 2101 tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt 2161 gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag 2221 caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat 2281 gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca 2341 ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt 2401 ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat 2461 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2521 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2581 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta ggatcgtaca 2641 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagaaag aggagaaata 2701 ctagatgacg agaaaatatc tcacacagga tgaagtctac aggctgatgg atgcagccca 2761 gagcatgtcc tttcctgaaa gaaatcgctg tctgattatg atggctttca ttcacggttt 2821 tagggccagt gaacttcttg atttacgttt atccgatatt gatgcatctg gaaaacaact 2881 taatattcgg cgtattaaaa atggtttctc gacaacacat cctcttcttc ctgatgaata 2941 taatttgatc aagttgtggc ttaagcagag gaagttaatt gaaaacggag ttgaaggaga 3001 ctggcttttt ctctcccgga aacgccgccc tatcagcaga caacattttt tttctatcat 3061 tcgtgaggct ggaaaacgtg caggattagc tgtaaaagca catcctcata tgttacgtca 3121 tgcctgtggt tttgctctgg ctgacaatgg tgtcgatacc cgactacttc aggattacct 3181 gggtcatcga aatattcagc atactgtcag atacacagcc agtaatgctg ctcgttttaa 3241 aggggtgtgg aaaaaaaagc ctcgt //