##gff-version 3
##sequence-region BBa_K1077002 1 3265
BBa_K1077002	.	engineered_region	1617	2678	.	+	0	ID=BBa_F2622;Name=BBa_F2622
BBa_K1077002	.	terminator	2487	2566	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2622
BBa_K1077002	.	stem_loop	2498	2541	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1077002	.	engineered_region	2487	2566	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1077002	.	CDS	1698	2453	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2622
BBa_K1077002	.	CDS	1698	2447	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1077002	.	engineered_region	1698	2453	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1077002	.	engineered_tag	2454	2478	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1077002	.	point_mutation	1871	1871	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1077002	.	engineered_region	1698	1699	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1077002	.	point_mutation	2189	2189	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1077002	.	promoter	2624	2678	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2622
BBa_K1077002	.	non_covalent_binding_site	2624	2643	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1077002	.	promoter	2665	2670	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1077002	.	engineered_region	2624	2678	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1077002	.	start_codon	2676	2676	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1077002	.	promoter	2643	2648	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1077002	.	promoter	1617	1670	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_F2622
BBa_K1077002	.	promoter	1636	1641	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1077002	.	promoter	1659	1664	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1077002	.	operator	1619	1635	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1077002	.	operator	1642	1658	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1077002	.	engineered_region	1617	1670	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1077002	.	ribosome_entry_site	1680	1691	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2622
BBa_K1077002	.	conserved_region	1684	1687	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1077002	.	terminator	2575	2615	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2622
BBa_K1077002	.	engineered_region	2575	2615	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1077002	.	stop_codon	2608	2608	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1077002	.	stem_loop	2582	2601	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1077002	.	polyA_site	2602	2615	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1077002	.	ribosome_entry_site	896	907	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1077002	.	conserved_region	900	903	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1077002	.	polypeptide_domain	2705	3265	.	+	0	ID=BBa_K880000;Name=BBa_K880000
BBa_K1077002	.	engineered_region	44	825	.	+	0	ID=BBa_K176001;Name=BBa_K176001
BBa_K1077002	.	terminator	785	825	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176001
BBa_K1077002	.	engineered_region	785	825	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1077002	.	stop_codon	818	818	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1077002	.	stem_loop	792	811	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1077002	.	polyA_site	812	825	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1077002	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176001
BBa_K1077002	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1077002	.	terminator	697	776	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176001
BBa_K1077002	.	stem_loop	708	751	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1077002	.	engineered_region	697	776	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1077002	.	CDS	62	688	.	+	0	ID=BBa_I732080;Name=BBa_I732080;Parent=BBa_K176001
BBa_K1077002	.	CDS	62	688	.	+	0	ID=annotation1940756;Name=tetR;Parent=BBa_I732080
BBa_K1077002	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1077002	.	ribosome_entry_site	2687	2698	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1077002	.	conserved_region	2691	2694	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1077002	.	promoter	834	887	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1077002	.	promoter	853	858	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1077002	.	non_covalent_binding_site	859	877	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1077002	.	engineered_region	834	887	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1077002	.	non_covalent_binding_site	834	852	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1077002	.	promoter	876	881	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1077002	.	terminator	1480	1608	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1077002	.	terminator	1480	1559	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1077002	.	stem_loop	1491	1534	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1077002	.	engineered_region	1480	1559	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1077002	.	terminator	1568	1608	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1077002	.	engineered_region	1568	1608	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1077002	.	stop_codon	1601	1601	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1077002	.	stem_loop	1575	1594	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1077002	.	polyA_site	1595	1608	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1077002	.	CDS	914	1471	.	+	0	ID=BBa_K137007;Name=BBa_K137007
>BBa_K1077002
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagt
gatagagatactgagcactactagagaaagaggagaaatactagatgatgcaggcggtttgttacggggcaacgggagc
cagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttgatctgcattatcaggac
cttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcacccgttacgttttgatg
agcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactgacgctatatttatttc
tcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattgaagctggaaccgtaacg
cagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagatactcgtttaattcagg
attatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgttttgccggattatggga
aagaaataatctcataaacgaaaaattaaaaagagaagaggtttaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaag
atactgagcacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaa
taaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatat
tatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatgga
ggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaa
ttggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatc
actgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaact
atatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaat
aaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaa
agctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaac
tcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaatta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagag
aaagaggagaaatactagatgacgagaaaatatctcacacaggatgaagtctacaggctgatggatgcagcccagagca
tgtcctttcctgaaagaaatcgctgtctgattatgatggctttcattcacggttttagggccagtgaacttcttgattt
acgtttatccgatattgatgcatctggaaaacaacttaatattcggcgtattaaaaatggtttctcgacaacacatcct
cttcttcctgatgaatataatttgatcaagttgtggcttaagcagaggaagttaattgaaaacggagttgaaggagact
ggctttttctctcccggaaacgccgccctatcagcagacaacattttttttctatcattcgtgaggctggaaaacgtgc
aggattagctgtaaaagcacatcctcatatgttacgtcatgcctgtggttttgctctggctgacaatggtgtcgatacc
cgactacttcaggattacctgggtcatcgaaatattcagcatactgtcagatacacagccagtaatgctgctcgtttta
aaggggtgtggaaaaaaaagcctcgt