BBa_I13500 1 BBa_I13500 Screening plasmid intermediate 2005-05-30T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 Built by Josh as an intermediate in screening plasmid construction false true _11_ 0 253 6 In stock false true jkm component1505002 1 BBa_E0040 component1505000 1 BBa_B0034 annotation1505002 1 BBa_E0040 range1505002 1 19 738 annotation1505000 1 BBa_B0034 range1505000 1 1 12 BBa_K1077000 1 fimIRL fim switch inverted repeat left IRL natural 2013-09-15T11:00:00Z 2015-05-08T01:09:03Z Synthesized based on E. coli CFT073 genomic sequence. fim switch inverted repeat left IRL natural. Ligate a part downstream, then ligate the IRR downstream of that. This switch half is in the "ON" orientation. false false _1386_ 0 13249 9 It's complicated true This switch half is in the "ON" orientation. false Mike Ferguson and Joshua Abramson BBa_K1077003 1 BBa_K1077003 J23100 fim switch b0034 GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:03Z none J23100 fim switch b0034 GFP false false _1386_ 0 13249 9 It's complicated true none false Mike Ferguson component2366531 1 BBa_I13500 component2366526 1 BBa_J23100 component2366527 1 BBa_K1077001 component2366525 1 BBa_K1077000 annotation2366527 1 BBa_K1077001 range2366527 1 343 379 annotation2366531 1 BBa_I13500 range2366531 1 388 1125 annotation2366525 1 BBa_K1077000 range2366525 1 1 291 annotation2366526 1 BBa_J23100 range2366526 1 300 334 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1077001 1 fimIRR fim switch inverted repeat right IRR natural 2013-09-15T11:00:00Z 2015-05-08T01:09:03Z Synthesized based on E. coli CFT073 genomic sequence fim switch inverted repeat right IRR natural. Ligate a part upstream, then ligate the IRL upstream of that. This switch half is in the "ON" orientation. false false _1386_ 0 13249 9 It's complicated true This switch half is in the "ON" orientation. false Mike Ferguson and Joshua Abramson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1077001_sequence 1 gatatagacagtttggccccaaatgtttcatcttttg BBa_B0034_sequence 1 aaagaggagaaa BBa_K1077003_sequence 1 gatttaacttattgataataaagttaaaaaacaaataaatacaagacaattggggccattttgactcatagaggaaagcatcgcgcacaaactttttcagtttatttgttggcttaatgttctatagttatctttatttgcagttttttatattgcatgaggtggtttttggagagaagaatgaggaagttgcgtcgagctacagaaacgttagctttacatatagcggaggtgatgtgaaattaatttacaagagaaataatttacatatcaaacagttagatgcttttttactagagttgacggctagctcagtcctaggtacagtgctagctactagaggatatagacagtttggccccaaatgtttcatcttttgtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_I13500_sequence 1 aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1077000_sequence 1 gatttaacttattgataataaagttaaaaaacaaataaatacaagacaattggggccattttgactcatagaggaaagcatcgcgcacaaactttttcagtttatttgttggcttaatgttctatagttatctttatttgcagttttttatattgcatgaggtggtttttggagagaagaatgaggaagttgcgtcgagctacagaaacgttagctttacatatagcggaggtgatgtgaaattaatttacaagagaaataatttacatatcaaacagttagatgcttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z