BBa_K864400 1 BBa_K864400 Ptac, trp & lac regulated promoter 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG Released HQ 2013 The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]] [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1124_ 0 9827 9 In stock false Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone. false Erik Lundin annotation2197240 1 lacO1 site range2197240 1 36 61 annotation2197238 1 -10 range2197238 1 29 33 annotation2197237 1 -35 range2197237 1 7 12 annotation2197236 1 Ptac range2197236 1 1 40 BBa_K1080003 1 "The Gun" GUN4 2013-09-11T11:00:00Z 2016-10-20T06:59:15Z Chlamydomonas reinhardtii Magnesium chelate co-factor false false _1389_ 7708 18463 9 It's complicated true Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence false Macquarie University annotation2525656 1 RBS range2525656 1 6 16 annotation2525657 1 GUN4 range2525657 1 25 675 BBa_K1080013 1 Gun4+ GUN4 (+ Ptac Promoter) 2013-09-21T11:00:00Z 2015-05-08T01:09:03Z Promoter - Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG GUN4 - Chlamydomonas reinhardtii Tetrapyrrole-binding protein - In Arabidopsis, GUN4 (Genomes uncoupled 4) is required for the functioning of the plastid mediated repression of nuclear transcription that is involved in controlling the levels of magnesium- protoporphyrin IX. GUN4 binds the product and substrate of Mg-chelatase, an enzyme that produces Mg-Proto, and activates Mg-chelatase. GUN4 is thought to participates in plastid-to-nucleus signaling by regulating magnesium-protoporphyrin IX synthesis or trafficking. This composite part contains the promoter BBa_K864400. The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1389_ 0 18612 9 Not in stock false Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence. Transformation done using Dh5-alpha competent cells. false Macquarie University component2364363 1 BBa_K1080003 component2364362 1 BBa_K864400 annotation2364362 1 BBa_K864400 range2364362 1 1 61 annotation2364363 1 BBa_K1080003 range2364363 1 70 797 BBa_K1080003_sequence 1 agctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgcct BBa_K1080013_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagagctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgcct BBa_K864400_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z