BBa_K864400
1
BBa_K864400
Ptac, trp & lac regulated promoter
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Created from oligos ordered from SigmaAldrich.
Oligo sequences:
Ptac+
AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA
Ptac-
CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
Released HQ 2013
The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]]
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
false
false
_1124_
0
9827
9
In stock
false
Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
false
Erik Lundin
annotation2197238
1
-10
range2197238
1
29
33
annotation2197237
1
-35
range2197237
1
7
12
annotation2197236
1
Ptac
range2197236
1
1
40
annotation2197240
1
lacO1 site
range2197240
1
36
61
BBa_K1080025
1
DVR1+
DVR1 (+ Ptac promoter)
2013-09-24T11:00:00Z
2015-05-08T01:09:03Z
See BBa_K1080012 and BBa_K864400
This composite combines a Ptac promoter with our gene for 3,8-divinyl protochlorophyllidea 8-vinyl reductase, DVR1.
false
false
_1389_
0
18612
9
Not in stock
false
See BBa_K1080012 and BBa_K864400
false
Macquarie University
component2364438
1
BBa_K1080012
component2364437
1
BBa_K864400
annotation2364438
1
BBa_K1080012
range2364438
1
70
1175
annotation2364437
1
BBa_K864400
range2364437
1
1
61
BBa_K1080012
1
DVR1
DVR1
2013-09-21T11:00:00Z
2015-05-08T01:09:03Z
Chlamydomonas reinhardtii
THSHERRNBHYDF
false
false
_1389_
0
18612
9
In stock
false
STHSHTSHD
false
Macquarie University
annotation2525687
1
RBS
range2525687
1
6
16
annotation2525688
1
DVR1
range2525688
1
25
1000
BBa_K1080012_sequence
1
agctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa
BBa_K1080025_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagagctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa
BBa_K864400_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z