LOCUS BBa_K1080026 9436 bp DNA linear 16-Dec-2024 DEFINITION Mg chelatase + Gun4 co-factor ACCESSION BBa_K1080026 VERSION BBa_K1080026.1 FEATURES Location/Qualifiers promoter 1..40 /label=Ptac promoter 7..12 /label=-35 promoter 29..33 /label=-10 misc_feature 36..61 /label=lacO1 site promoter 1..61 /label=BBa_K864400 RBS 75..85 /label=RBS CDS 94..1182 /label=ChlI1 CDS 70..1185 /label=BBa_K1080000 RBS 1199..1209 /label=RBS CDS 1218..2399 /label=ChlI2 CDS 1194..2405 /label=BBa_K1080011 RBS 2419..2429 /label=RBS CDS 2438..6535 /label=ChlH CDS 2414..6538 /label=BBa_K1080001 RBS 6552..6562 /label=RBS CDS 6571..8700 /label=ChlD CDS 6547..8700 /label=BBa_K1080002 RBS 8714..8724 /label=RBS CDS 8733..9383 /label=GUN4 CDS 8709..9436 /label=BBa_K1080003 ORIGIN 1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat 61 ttactagaga gctttaagaa ggagatatac ataatggcag ctactgaggt caaggctgct 121 gaaggccgta ctgagaagga gctgggtcag gcacgtccaa tctttccatt caccgccatc 181 gttggtcagg atgagatgaa gctggcgctg attctgaacg tgatcgatcc taagatcggt 241 ggtgtcatga tcatgggtga ccgtggtact ggtaagtcca ctactattcg tgcactggca 301 gatctgctgc ctgaaatgca ggtggttgct aacgatccat ttaactcgga tccaactgat 361 ccagagctga tgagtgaaga agttcgtaac cgtgtcaagg caggtgaaca gctgccagtg 421 tcttccaaga agattcctat ggttgacctg ccactgggtg ctactgaaga tcgtgtgtgc 481 ggtaccatcg atatcgagaa ggcactgact gaaggtgtta aggcattcga gcctggtctg 541 ctggctaagg ctaaccgtgg tatcctgtac gtggatgaag tcaacctgct ggatgatcat 601 ctggttgatg ttctgctgga ctcggcagca tctggttgga acaccgtgga gcgtgaaggt 661 atctccatca gccacccagc ccgcttcatc ctggttggtt cgggtaaccc agaggaaggt 721 gaactgcgtc cacagctgct ggatcgcttc ggcatgcacg ctcagatcgg taccgtcaaa 781 gatcctcgtc tgcgtgtgca aatcgtgtcg cagcgctcga ccttcgacga gaatccagcc 841 gcctttcgca aggactacga agctggtcag atggcactga cacagcgtat cgttgacgct 901 cgtaagctgc tgaagcaagg tgaagtcaac tacgacttcc gcgtcaagat cagccagatc 961 tgctcggacc tgaacgtgga cggcatccgt ggtgatatcg tgactaaccg tgcagctaag 1021 gctctggctg cattcgaagg tcgtaccgaa gtgactccag aagacatcta ccgtgtcatt 1081 ccactgtgcc tgcgtcaccg tctccgtaaa gacccactgg ctgagatcga cgacggtgac 1141 cgtgtgcgtg agatcttcaa gcaggtgttc ggcatggagt aataatacta gagagcttta 1201 agaaggagat atacataatg ccatccacga aagcagctaa gaagccgaac ttcccattcg 1261 tcaagattca aggtcaagag gaaatgaagc ttgcactgct gctgaacgtt gttgatccaa 1321 acatcggtgg tgtacttatt atgggtgacc gtggtactgc taagtcggtc gcagtccgtg 1381 ctctggtgga tatgcttcca gacattgacg tggttgaagg tgacgcattc aacagctctc 1441 caacagatcc taagttcatg ggtccagaca cactgcaacg tttccgtaac ggcgagaagc 1501 tgccaaccgt tcgtatgcgg acaccactgg ttgaactgcc tctgggtgca accgaggatc 1561 gtatctgcgg tacaatcgat atcgagaagg cactgacgca gggtatcaag gcatacgaac 1621 ctggtctgct ggcaaaggct aaccgtggta tcctgtatgt ggacgaagtg aacctgctgg 1681 atgatggtct ggttgatgtc gtgctggact cgtcggctag cggtctgaac actgtggaac 1741 gtgaaggtgt gtctattgtg catcctgcac gtttcatcat gattggttca ggtaaccccc 1801 aggaaggtga actgcgtcca cagctgctgg atcgtttcgg catgagcgtc aacgtggcaa 1861 cgctgcaaga caccaagcaa cgtacgcagc tggtgctgga tcgtcttgcg tacgaagctg 1921 accctgacgc atttgtggac tcgtgcaagg cagagcagac agcactcacg gacaagctgg 1981 aagcagcacg tcaacgtctg cgttccgtca agatcagcga agaactgcaa atcctgatct 2041 ctgacatttg ctctcgtctg gatgttgatg gtctgcgtgg tgacattgtt atcaaccgtg 2101 ctgctaaggc tcttgttgca ttcgaaggtc gtaccgaagt taccacgaat gacgtggaac 2161 gtgtcatctc gggttgcctc aaccatcgtc tgcgtaagga cccactggac cccattgaca 2221 acggcactaa ggttgcaatc ctgttcaagc gtatgactga tccagaaatc atgaagcgtg 2281 aagaagaagc taagaagaag cgcgaagagg ctgctgccaa ggctaaggct gaaggtaagg 2341 ctgatcgtcc tacgggtgct aaggctggtg catgggctgg cttgccacct cgtcggtaat 2401 aataatacta gagagcttta agaaggagat atacataatg tgcaatgtgg cgactggacc 2461 acgtccacca atgaccacct tcacaggtgg caacaagggt cctgctaagc agcaggtgtc 2521 gctggatctg cgtgacgatg gtgctggcat gttcaccagc accagcccag agatgcgtcg 2581 tgtcgtccct gacgatgtga agggtcgcgt taaggtgaag gttgtgtacg tggtgctgga 2641 ggcacagtac cagtcggcaa tcagcgctgc tgtgaagaac atcaacgcca agaactccaa 2701 ggtgtgcttc gaggtggtgg gctacctgct ggaggagctg cgtgaccaga agaacctcga 2761 tatgctcaag gaggatgtgg cctctgccaa catcttcatc ggctcgctca tcttcattga 2821 ggagcttgcc gagaagattg tggaggcggt gagccccctg cgcgagaagc tggacgcgtg 2881 cctgatcttc ccgtccatgc cggcggtcat gaagctgaac aagctgggca cgttttcgat 2941 ggctcagctg ggccagtcga agtcggtgtt ctcggagttc atcaagtctg ctcgcaagaa 3001 caacgacaac ttcgaggagg gcttgctgaa gctggtgcgc accctgccta aggtgctgaa 3061 gtatctgccc tcggacaagg cgcaggacgc caagaacttc gtgaacagcc tgcaatactg 3121 gctgggtggt aactcggaca acctggagaa cctgctgctg aacaccgtca gcaactacgt 3181 gccagctctg aagggtgtgg acttcagcgt ggctgagccc accgcatacc cagatgtggg 3241 tatctggcac cctctggcct cgggtatgta cgaggacctg aaggagtacc tgaactggta 3301 cgacacccgt aaggacatgg tcttcgccaa ggacgcaccc gtcattggcc tggtgctgca 3361 acgctcgcac ctggtgactg gcgatgaggg tcactacagc ggtgtggtcg ctgagctgga 3421 gagccgtggt gctaaggtca tcccagtctt tgcaggtggc ctggacttct ccgcacccgt 3481 caagaagttc ttctacgacc cactgggctc tggtcgcacg ttcgtggaca ccgttgtgtc 3541 gctgaccggt ttcgcactgg tgggtggtcc agcacgtcag gacgcaccga aggccattga 3601 ggcactgaag aacctgaacg tgccctacct ggtgtcgctg ccgctggtgt tccagaccac 3661 tgaggagtgg ctggacagcg agctgggtgt gcacccagtc caggtggctc tgcaagttgc 3721 cctgccagag ctggatggtg ccatggagcc catcgtgttc gctggtcgtg actcgaacac 3781 aggcaagtcg cactcgctgc cagaccgcat cgcttcgctg tgcgctcgtg cagtgaactg 3841 ggcaaacctg cgcaagaagc gcaacgccga gaagaagctg gcagtcaccg tgttcagctt 3901 cccacctgac aagggcaacg tcggcactgc tgcctacctg aacgtgttcg gctccatcta 3961 ccgtgtgctg aagaatctgc aacgtgaggg ctacgacgtg ggtgccctgc caccctcgga 4021 ggaggatctg atccagtcgg tgctgaccca gaaggaggcc aagttcaact cgaccgacct 4081 gcacatcgcc tacaagatga aggtggacga gtaccagaag ctgtgccctt acgccgaggc 4141 gctggaggag aactggggca agccccccgg caccctgaac accaacggcc aggagctgct 4201 ggtgtacggc cgccagtacg gcaacgtctt catcggcgtg cagcccacct tcggctacga 4261 gggcgacccg atgcgcctgc tgttctcgaa gtcggccagc ccccaccacg gcttcgccgc 4321 ctactacacc ttcctggaga agatcttcaa ggccgacgcc gtgctgcact tcggcaccca 4381 cggctcgctg gagttcatgc ccggcaagca ggtcggcatg tcgggtgtgt gctaccccga 4441 ctcgctgatc ggcaccatcc ccaacctcta ctactacgcc gccaacaacc cgtctgaggc 4501 caccatcgcc aagcgccgct cgtacgccaa caccatttcg tacctgacgc cgcctgccga 4561 gaacgccggc ctgtacaagg gcctgaagga gctgaaggag ctgatcagct cgtaccaggg 4621 catgcgtgag tctggccgcg ccgagcagat ctgcgccacc atcattgaga ccgccaagct 4681 gtgcaacctg gaccgcgacg tgaccctgcc cgacgctgac gccaaggacc tgaccatgga 4741 catgcgcgac agcgttgtgg gccaggtgta ccgcaagctg atggagattg agtcccgcct 4801 gctgccctgc ggcctgcacg tggtgggctg cccgcccacc gccgaggagg ccgtggccac 4861 cctggtcaac atcgctgagc tggaccgccc ggacaacaac ccccccatca agggcatgcc 4921 cggcatcctg gcccgcgcca ttggtcgcga catcgagtcg atttacagcg gcaacaacaa 4981 gggtgtcctg gctgacgttg accagctgca acgcatcacc gaggcctccc gtacctgcgt 5041 gcgtgagttc gtgaaggacc gcaccggtct gaacggtcgt atcggcacca actggatcac 5101 caacctgctc aagttcaccg gtttctacgt ggacccctgg gtgcgtggtc tgcaaaacgg 5161 tgagttcgcc agcgcaaacc gtgaggagct gatcaccctg ttcaactacc tggagttctg 5221 cctgacccag gtggtcaagg acaacgagct gggtgcactg gtagaggcac tgaacggtca 5281 gtacgtcgag ccaggtccag gtggtgaccc catccgcaac cccaacgtgc tgcccaccgg 5341 taagaacatc cacgcactgg accctcagtc gattcccact caggcagcac tgaagagcgc 5401 acgtctggtg gtggaccgtc tgctggaccg tgagcgcgac aacaacggcg gcaagtaccc 5461 cgagaccatc gcgctggtgc tgtggggcac tgacaacatc aagacctacg gcgagtcgct 5521 ggcccaggtc atgatgatgg tcggtgtcaa gcccgtggcc gacgccctgg gtcgtgtgaa 5581 caagctggag gtgatccctc tggaggagct gggtcgtcca cgtgtggacg tggttgtcaa 5641 ctgctcgggt gtgttccgcg acctgttcgt gaaccagatg ctgctgctgg accgtgcaat 5701 caagctggca gcagagcagg acgagccaga tgagatgaac ttcgtgcgca agcacgccaa 5761 gcagcaggca gcagagctgg gtctgcaaag cctgcgcgac gcagcaaccc gtgtgttctc 5821 caacagctcg ggttcctact cgtccaacgt caacctggcg gtggagaaca gcagctggag 5881 cgacgagtcg cagctgcaag agatgtacct gaagcgcaag tcgtacgcat tcaactcgga 5941 ccgtccaggt gcaggtggtg agatgcagcg cgacgtgttc gagacggcaa tgaagaccgt 6001 ggacgtgacc ttccagaacc tggactcgtc cgagatctcg ctgaccgatg tgtcgcacta 6061 cttcgactct gacccaacca agctggttgc atcgcttcgt aacgatggtc gtacacctaa 6121 cgcatacatc gcagacacca ctactgctaa cgcgcaggtc cgcactcttg gtgagactgt 6181 gcgtctggac gcacgcacca agctgctcaa tcctaagtgg tacgaaggaa tgcttgcctc 6241 aggatacgaa ggagtgcgcg agatccagaa gcgcatgacc aacaccatgg gatggtctgc 6301 tacctcggga atggtggaca actgggtgta cgacgaggct aactcgacct tcatcgagga 6361 tgcggccatg gctgagcgcc tgatgaacac caaccctaac agcttccgca agctggtggc 6421 taccttcctg gaagccaacg gacgcggcta ctgggatgct aagcctgagc agctggaacg 6481 tctgcgtcag ctgtacatgg acgtggagga caagattgag ggagtcgaat aataataata 6541 ctagagagct ttaagaagga gatatacata atgcgcgcca tgaaggtgtc tgaggaggac 6601 tccaagggct tcgatgcgga tgtgtcgacc cgcctggccc gctcgtaccc tctggcggcc 6661 gtggtgggcc aggacaacat caagcaggcg ctgctgctgg gcgccgtgga caccgggctg 6721 ggcggcatcg ccatcgccgg tcgccgcggt accgccaagt ccatcatggc tcgcggcctg 6781 cacgctctgc tgccgcccat tgaggtggtg gagggcagca tctgcaacgc cgaccccgag 6841 gacccccgct cctgggaggc tggcctggct gagaagtatg cgggcggccc tgtgaagacc 6901 aagatgcgct cggcgccgtt tgtgcagatc cctctgggtg tgactgagga ccgcttggtg 6961 ggcactgtgg acattgaggc gtccatgaag gagggcaaga ctgtgttcca gcccggcctg 7021 ctggctgagg cgcaccgcgg catcctgtac gtggacgaga tcaacctgct ggatgacggc 7081 attgccaacc tgctgctgtc catcctgtcg gacggagtca acgtggtgga gcgcgagggc 7141 atctccatca gccacccctg ccggccgctg ctgattgcca cctacaaccc cgaggagggc 7201 cctctgcgtg agcacctgct ggaccgcatc gccattggcc tcagcgccga cgtccccagc 7261 accagcgacg agcgcgtcaa ggccattgac gcagccatcc gcttccagga caagccgcag 7321 gacactattg acgacaccgc ggagctcacc gacgccctgc gcacctcggt catcctggct 7381 cgcgagtacc tgaaggacgt gaccatcgcg ccggagcagg tgacctacat tgtggaggag 7441 gcgcgccgcg gcggagtcca ggggcaccgc gcggagctgt acgcggtcaa gtgtgccaag 7501 gcgtgtgcgg ctctggaggg ccgtgagcgt gtgaacaagg atgacctgcg ccaggccgtg 7561 cagctggtca tcctgccgcg cgccaccatc ctggaccagc ccccgcccga gcaggagcag 7621 cccccgccgc cgcccccgcc ccctcccccg ccgccgccgc aggaccaaat ggaggacgag 7681 gaccaggagg agaaggagga cgagaaggag gaggaggaga aggagaacga ggaccaggac 7741 gagcccgaga tccctcagga gttcatgttt gagtccgagg gcgtcatcat ggacccctcc 7801 atcctcatgt tcgcgcagca gcagcagcgc gcgcagggcc gctccggccg cgccaagacg 7861 ctcatcttca gcgacgaccg cggccgctac atcaagccca tgctgcccaa gggtgacaag 7921 gtcaagcgcc tggcagtgga cgccacgctt cgcgccgccg cgccctacca gaagattcgc 7981 cggcagcagg ccatcagcga gggcaaggtg cagcgcaagg tgtacgtgga caagccagac 8041 atgcgctcca agaagctggc ccgcaaggcc ggtgcgctgg tgatttttgt tgtggacgcg 8101 tccggctcca tggctctgaa ccgcatgagc gccgccaagg gcgcctgcat gcgcctgctg 8161 gctgagtcgt acaccagccg cgaccaggtg tgcctcatcc ccttctacgg cgacaaggcc 8221 gaggtgctgc tgccgccctc caagtccatc gccatggccc gccgccgcct ggactcgctg 8281 ccctgcggcg gcggctcgcc ccttgcgcac ggcctgtcca cggcggtacg tgtgggcatg 8341 caggccagcc aggcgggcga ggtgggccgc gtcatgatgg tgctcatcac ggacggccgc 8401 gccaacgtca gcctggccaa gtccaacgag gaccccgagg cgctcaagcc cgacgcgccc 8461 aagcccaccg ccgactcgct gaaggacgag gtgcgcgaca tggccaagaa ggccgcgtcc 8521 gccggcatca acgtgcttgt cattgacacg gagaacaagt tcgtgagcac cggctttgcg 8581 gaggagatct ccaaggcagc gcagggcaag tactactacc tgcccaacgc cagcgacgca 8641 gcaatcgcag cagcagcatc cggtgcaatg gcagcagcta agggaggata ctagcaataa 8701 tactagagag ctttaagaag gagatataca taatggcgat gcgtgtcacc gtcgctgctg 8761 gtaagctgga ctctgtctcc ctgttcggtg gagacaccgc cagcctgatg ggtggttcac 8821 agactgtgga gaagaagaag tctggtaagg aggctgtgat ggaggtgcag ctcagctcca 8881 cagcaggcat cgactacacc gtccttcgtg accaccttgc caacggtgag ttccgtgagg 8941 cagaggacga gactcgtgct ctgctgatca agcttgctgg tccagaagca gtcaagcgca 9001 actgggttta cttcaccgag gtgaagaaca tttccgtcac cgatttccag accctggaca 9061 acctgtggaa ggcgtccagc aacaacaagt tcggctacag cgttcagaag gagatctggg 9121 tgcagaacca gaagcgctgg cccaagttct tcaagcagat tgactggacc cagggcgaga 9181 acaacaacta ccggaagtgg cccatggagt tcatctacag catggacgcc ccccgcggcc 9241 acctgcccct caccaacgcg ctccgaggga cgcagctgtt ccaggcgatc atggagcacc 9301 cagcgttcga gaagtccagc acggcaaaga cgcttgatca gaaggcggcg gaggccgccg 9361 ggcggacaca gtcgctgttc taagctctca gcagctcagc ggctgatcct gccaggtgtt 9421 gtatcagcgg ccgcct //