BBa_K1083001
1
BBa_K1083001
HEWL-Antigen-Luciferase
2013-09-26T11:00:00Z
2015-05-08T01:09:04Z
This sequence comes from cameloids.
This generates a protein construct containing a epitope for HEWL (see biobrick BBa_K1083000). The HEWL epitope (V) is exchangebla due to a built in restriction site for XhoI. H and CH2 are optimezed for E.coli. The VHCH2 generates an antibody from cameloids. This part is connected to Lucifarese (biobrick BBa_K3255209).
false
false
_1393_
0
16112
9
It's complicated
false
This part was very tricky to design due to allot of restriction sites high gc content and not optimized for e. coli.
false
David Jullesson
annotation2364601
1
HEWL-Antibody
range2364601
1
1
1075
annotation2364602
1
Luciferase
range2364602
1
1076
2693
BBa_K1083001_sequence
1
aaagaggagaatactagatggaactgggtctgtcttgggttgttctggcagcactgcttcaggatgttcagctggttgaatctggtggtggtagcgttcaggcaggtggttctctgcgtctgtcttgcgcagtttctggctataaagatcgcaactattgcatgggctggtttcgccgtgcaccgggcaaagaacgcgaaggcgttgcggtgattgatagcagcggcctcaccgcgtatgcggatagcgtggaactcgagattagcgaaccgcaaccgcaaccgggttgcacctgtccgaaatgtccggcaccggaactgccgggtggtccgtctgtgtttgtgtttccgccgaaaccgaaagatgtgctgagcattagcggtcgcccggaagtgacctgcgtggtggtggatgtgggccaggaagatccggaagtgaactttaactggtatattgatggcgtggaagtgcgcaccgcgaacaccaaaccgaaagaagaacagtttaacagcacctatcgcgtggtgagcgtgctgaccattcagcatcaggattggctgaccggcaaagaatttaaatgcaaagtgaacaacaaagcgctgccggcgccgattgaacgcaccattagcaaagcgaaaggccagacccgcgaaccgcaggtgtataccctggcgccgcatcgcgaagaactggcgaaagataccgtgaacgtgacctgcctggtgaaaggcttttatccgccggatattaacgtggaatggcagcgcaacggccagccggaaagcgaaggcgcgtatgcgaccaccctgccgcagcaggataacgatggcacctattttctgtatagcaaactgagcgtgggcaaaaacacctggcagcagggcgaaacctttacctgcgtggtgatgcatgaagcgctgcataaccatagcacccagaaaagcattacccagagcagcggcaaaagtcctaacggcgcgagcgacagtggttctgcttcgaagactggttctgcttcgcggatccttaaggaggtaaaaaaaatggagaacatggagaacgacgagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatggaacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttggaaaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaactgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgctgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttattacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgcctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaagaacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgttacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtcccttttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttgatgaagaaaccttcctgaaaaccctgcaagactacaagtgcactagtgtgattctggtcccgacgctgttcgcgatcctgaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaagaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcgcgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttattgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggttatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacgatgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcggagctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaactgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaagtgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacggtcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z