LOCUS       BBa_K1085010             907 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1
ACCESSION   BBa_K1085010
VERSION     BBa_K1085010.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=RBS
     CDS             17..649
                     /label=estA (Bac. sub.)
     misc_feature    656..679
                     /label=strep
     CDS             698..907
                     /label=spidersilk subunit E1
     misc_feature    14..16
                     /label=starting codon (ATG)
ORIGIN
        1 aggaggcata tccatgaaat ttgtaaaaag aaggatcatt gcacttgtaa caattttgat
       61 gctgtctgtt acatcgctgt ttgcgttgca gccgtcagca aaagccgctg aacacaatcc
      121 agtcgttatg gttcacggta ttggaggggc atcattcaat tttgcgggaa ttaagagcta
      181 tctcgtatct cagggctggt cgcgggacaa gctgtatgca gttgattttt gggacaagac
      241 aggcacaaat tataacaatg gaccggtatt atcacgattt gtgcaaaagg ttttagatga
      301 aacgggtgcg aaaaaagtgg atattgtcgc tcacagcatg gggggcgcga acacacttta
      361 ctacataaaa aatctggacg gcggaaataa agttgcaaac gtcgtgacgc ttggcggcgc
      421 gaaccgtttg acgacaggca aggcgcttcc gggaacagat ccaaatcaaa agattttata
      481 cacatccatt tacagcagtg ccgatatgat tgtcatgaat tacttatcaa gattagatgg
      541 tgctagaaac gttcaaatcc atggcgttgg acacatcggc cttctgtaca gcagccaagt
      601 caacagcctg attaaagaag ggctgaacgg cgggggccag aatacgaatg gatcctggag
      661 ccatccgcag tttgaaaaat ccgcagcagc atccgcaggc ggatacggac caggagcagg
      721 ccaacaaggc ccaggctcac aaggcccagg ctcaggcgga caacaaggac caggcggaca
      781 aggcggctac ggaccaggcg ccggacaaca aggcccaggc agccaaggac caggcagcgg
      841 cggccaacaa ggcccaggag gacaaggacc atacggccca agcgcagcag ccgcagcagc
      901 cgcagcc
//