LOCUS BBa_K1085018 767 bp DNA linear 22-Nov-2024 DEFINITION RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 SilkTail Strep-tag stop-codon ACCESSION BBa_K1085018 VERSION BBa_K1085018.1 FEATURES Location/Qualifiers RBS 1..6 /label=RBS CDS 17..226 /label=spidersilk subunit E1 misc_feature 14..16 /label=starting codon (ATG) CDS 1..226 /label=BBa_K1085001 CDS 235..444 /label=spidersilk subunit E2 CDS 235..444 /label=BBa_K1085002 misc_feature 741..764 /label=strep CDS 453..722 /label=spidersilk C-terminal domain misc_feature 765..767 /label=Stop codon (TGA) misc_feature 453..767 /label=BBa_K1085009 ORIGIN 1 aggaggcata tccatgggcg gatacggacc aggagcaggc caacaaggcc caggctcaca 61 aggcccaggc tcaggcggac aacaaggacc aggcggacaa ggcggctacg gaccaggcgc 121 cggacaacaa ggcccaggca gccaaggacc aggcagcggc ggccaacaag gcccaggagg 181 acaaggacca tacggcccaa gcgcagcagc cgcagcagcc gcagcctact agagggcgga 241 tacggcccag gcgcaggcca acaaggacca ggcagccaag gaccaggctc aggcggacaa 301 caaggcccag gcggacaagg cggctacggc ccaggcgccg gacaacaagg accaggctca 361 caaggaccag gctccggcgg acaacaagga ccaggcggcc aaggaccata cggaccaagc 421 gcagcagccg cagcagccgc cgcatactag aggccccagt tgcctccgca gccgcctcaa 481 ggctgtcctc ccctcaagcc tcctcccgtg tttcatccgc cgtgtccact ctcgtgtcct 541 ccggacctac gaatcctgcc gccttatcca atgccatctc cagcgttgta tcacaagttt 601 cagcctccaa tcctggacta tccggatgtg acgttctcgt tcaagccctc ctcgaactcg 661 tatccgccct cgtacacatc ctcggctcct cctccattgg acaaattaat tacgccgcct 721 cctccgcagc agcatccgca tggagccatc cgcagtttga aaaatga //