LOCUS       BBa_K1085018             767 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 SilkTail 
            Strep-tag stop-codon
ACCESSION   BBa_K1085018
VERSION     BBa_K1085018.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=RBS
     CDS             17..226
                     /label=spidersilk subunit E1
     misc_feature    14..16
                     /label=starting codon (ATG)
     CDS             1..226
                     /label=BBa_K1085001
     CDS             235..444
                     /label=spidersilk subunit E2
     CDS             235..444
                     /label=BBa_K1085002
     misc_feature    741..764
                     /label=strep
     CDS             453..722
                     /label=spidersilk C-terminal domain
     misc_feature    765..767
                     /label=Stop codon (TGA)
     misc_feature    453..767
                     /label=BBa_K1085009
ORIGIN
        1 aggaggcata tccatgggcg gatacggacc aggagcaggc caacaaggcc caggctcaca
       61 aggcccaggc tcaggcggac aacaaggacc aggcggacaa ggcggctacg gaccaggcgc
      121 cggacaacaa ggcccaggca gccaaggacc aggcagcggc ggccaacaag gcccaggagg
      181 acaaggacca tacggcccaa gcgcagcagc cgcagcagcc gcagcctact agagggcgga
      241 tacggcccag gcgcaggcca acaaggacca ggcagccaag gaccaggctc aggcggacaa
      301 caaggcccag gcggacaagg cggctacggc ccaggcgccg gacaacaagg accaggctca
      361 caaggaccag gctccggcgg acaacaagga ccaggcggcc aaggaccata cggaccaagc
      421 gcagcagccg cagcagccgc cgcatactag aggccccagt tgcctccgca gccgcctcaa
      481 ggctgtcctc ccctcaagcc tcctcccgtg tttcatccgc cgtgtccact ctcgtgtcct
      541 ccggacctac gaatcctgcc gccttatcca atgccatctc cagcgttgta tcacaagttt
      601 cagcctccaa tcctggacta tccggatgtg acgttctcgt tcaagccctc ctcgaactcg
      661 tatccgccct cgtacacatc ctcggctcct cctccattgg acaaattaat tacgccgcct
      721 cctccgcagc agcatccgca tggagccatc cgcagtttga aaaatga
//