LOCUS BBa_K1085020 1298 bp DNA linear 06-Nov-2024 DEFINITION RBS Start-codon EstA SpiderSilkSubunitN1 SpiderSilkSubunitN2 SilkTail Strep-tag Stop-codon ACCESSION BBa_K1085020 VERSION BBa_K1085020.1 FEATURES Location/Qualifiers RBS 1..6 /label=RBS misc_feature 14..16 /label=starting codon (ATG) CDS 17..649 /label=estA (Bac. sub.) misc_feature 656..679 /label=strep CDS 698..838 /label=spidersilk subunit N1 CDS 1..838 /label=BBa_K1085012 CDS 847..975 /label=spidersilk subunit N2 CDS 847..975 /label=BBa_K1085006 misc_feature 1272..1295 /label=strep CDS 984..1253 /label=spidersilk C-terminal domain misc_feature 1296..1298 /label=Stop codon (TGA) misc_feature 984..1298 /label=BBa_K1085009 ORIGIN 1 aggaggcata tccatgaaat ttgtaaaaag aaggatcatt gcacttgtaa caattttgat 61 gctgtctgtt acatcgctgt ttgcgttgca gccgtcagca aaagccgctg aacacaatcc 121 agtcgttatg gttcacggta ttggaggggc atcattcaat tttgcgggaa ttaagagcta 181 tctcgtatct cagggctggt cgcgggacaa gctgtatgca gttgattttt gggacaagac 241 aggcacaaat tataacaatg gaccggtatt atcacgattt gtgcaaaagg ttttagatga 301 aacgggtgcg aaaaaagtgg atattgtcgc tcacagcatg gggggcgcga acacacttta 361 ctacataaaa aatctggacg gcggaaataa agttgcaaac gtcgtgacgc ttggcggcgc 421 gaaccgtttg acgacaggca aggcgcttcc gggaacagat ccaaatcaaa agattttata 481 cacatccatt tacagcagtg ccgatatgat tgtcatgaat tacttatcaa gattagatgg 541 tgctagaaac gttcaaatcc atggcgttgg acacatcggc cttctgtaca gcagccaagt 601 caacagcctg attaaagaag ggctgaacgg cgggggccag aatacgaatg gatcctggag 661 ccatccgcag tttgaaaaat ccgcagcagc atccgcacca ggaggagccg gctaccaagg 721 accaggcggc taccaaggac catacggacc aggcggcggc tacggaccag gcgcaggcta 781 ccaaggacca ggctcacaat acggaccagg ctcagcagca gccgcagcag ccgcagcata 841 ctagagggct accaaggacc aggcggctac caaggaccat acggaccagg cggcggctac 901 ggaccaggcg caggctacca aggaccaggc tcacaatacg gaccaggctc agcagcagcc 961 gcagcagccg cagcatacta gaggccccag ttgcctccgc agccgcctca aggctgtcct 1021 cccctcaagc ctcctcccgt gtttcatccg ccgtgtccac tctcgtgtcc tccggaccta 1081 cgaatcctgc cgccttatcc aatgccatct ccagcgttgt atcacaagtt tcagcctcca 1141 atcctggact atccggatgt gacgttctcg ttcaagccct cctcgaactc gtatccgccc 1201 tcgtacacat cctcggctcc tcctccattg gacaaattaa ttacgccgcc tcctccgcag 1261 cagcatccgc atggagccat ccgcagtttg aaaaatga //