LOCUS       BBa_K1085020            1298 bp    DNA     linear       25-Nov-2024
DEFINITION  RBS Start-codon EstA SpiderSilkSubunitN1 SpiderSilkSubunitN2 
            SilkTail Strep-tag Stop-codon
ACCESSION   BBa_K1085020
VERSION     BBa_K1085020.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=RBS
     misc_feature    14..16
                     /label=starting codon (ATG)
     CDS             17..649
                     /label=estA (Bac. sub.)
     misc_feature    656..679
                     /label=strep
     CDS             698..838
                     /label=spidersilk subunit N1
     CDS             1..838
                     /label=BBa_K1085012
     CDS             847..975
                     /label=spidersilk subunit N2
     CDS             847..975
                     /label=BBa_K1085006
     misc_feature    1272..1295
                     /label=strep
     CDS             984..1253
                     /label=spidersilk C-terminal domain
     misc_feature    1296..1298
                     /label=Stop codon (TGA)
     misc_feature    984..1298
                     /label=BBa_K1085009
ORIGIN
        1 aggaggcata tccatgaaat ttgtaaaaag aaggatcatt gcacttgtaa caattttgat
       61 gctgtctgtt acatcgctgt ttgcgttgca gccgtcagca aaagccgctg aacacaatcc
      121 agtcgttatg gttcacggta ttggaggggc atcattcaat tttgcgggaa ttaagagcta
      181 tctcgtatct cagggctggt cgcgggacaa gctgtatgca gttgattttt gggacaagac
      241 aggcacaaat tataacaatg gaccggtatt atcacgattt gtgcaaaagg ttttagatga
      301 aacgggtgcg aaaaaagtgg atattgtcgc tcacagcatg gggggcgcga acacacttta
      361 ctacataaaa aatctggacg gcggaaataa agttgcaaac gtcgtgacgc ttggcggcgc
      421 gaaccgtttg acgacaggca aggcgcttcc gggaacagat ccaaatcaaa agattttata
      481 cacatccatt tacagcagtg ccgatatgat tgtcatgaat tacttatcaa gattagatgg
      541 tgctagaaac gttcaaatcc atggcgttgg acacatcggc cttctgtaca gcagccaagt
      601 caacagcctg attaaagaag ggctgaacgg cgggggccag aatacgaatg gatcctggag
      661 ccatccgcag tttgaaaaat ccgcagcagc atccgcacca ggaggagccg gctaccaagg
      721 accaggcggc taccaaggac catacggacc aggcggcggc tacggaccag gcgcaggcta
      781 ccaaggacca ggctcacaat acggaccagg ctcagcagca gccgcagcag ccgcagcata
      841 ctagagggct accaaggacc aggcggctac caaggaccat acggaccagg cggcggctac
      901 ggaccaggcg caggctacca aggaccaggc tcacaatacg gaccaggctc agcagcagcc
      961 gcagcagccg cagcatacta gaggccccag ttgcctccgc agccgcctca aggctgtcct
     1021 cccctcaagc ctcctcccgt gtttcatccg ccgtgtccac tctcgtgtcc tccggaccta
     1081 cgaatcctgc cgccttatcc aatgccatct ccagcgttgt atcacaagtt tcagcctcca
     1141 atcctggact atccggatgt gacgttctcg ttcaagccct cctcgaactc gtatccgccc
     1201 tcgtacacat cctcggctcc tcctccattg gacaaattaa ttacgccgcc tcctccgcag
     1261 cagcatccgc atggagccat ccgcagtttg aaaaatga
//