LOCUS BBa_K1085023 1125 bp DNA linear 06-Nov-2024 DEFINITION RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2 ACCESSION BBa_K1085023 VERSION BBa_K1085023.1 FEATURES Location/Qualifiers RBS 1..6 /label=RBS CDS 17..649 /label=estA (Bac. sub.) misc_feature 656..679 /label=strep CDS 698..907 /label=spidersilk subunit E1 misc_feature 14..16 /label=starting codon (ATG) CDS 1..907 /label=BBa_K1085010 CDS 916..1125 /label=spidersilk subunit E2 CDS 916..1125 /label=BBa_K1085002 ORIGIN 1 aggaggcata tccatgaaat ttgtaaaaag aaggatcatt gcacttgtaa caattttgat 61 gctgtctgtt acatcgctgt ttgcgttgca gccgtcagca aaagccgctg aacacaatcc 121 agtcgttatg gttcacggta ttggaggggc atcattcaat tttgcgggaa ttaagagcta 181 tctcgtatct cagggctggt cgcgggacaa gctgtatgca gttgattttt gggacaagac 241 aggcacaaat tataacaatg gaccggtatt atcacgattt gtgcaaaagg ttttagatga 301 aacgggtgcg aaaaaagtgg atattgtcgc tcacagcatg gggggcgcga acacacttta 361 ctacataaaa aatctggacg gcggaaataa agttgcaaac gtcgtgacgc ttggcggcgc 421 gaaccgtttg acgacaggca aggcgcttcc gggaacagat ccaaatcaaa agattttata 481 cacatccatt tacagcagtg ccgatatgat tgtcatgaat tacttatcaa gattagatgg 541 tgctagaaac gttcaaatcc atggcgttgg acacatcggc cttctgtaca gcagccaagt 601 caacagcctg attaaagaag ggctgaacgg cgggggccag aatacgaatg gatcctggag 661 ccatccgcag tttgaaaaat ccgcagcagc atccgcaggc ggatacggac caggagcagg 721 ccaacaaggc ccaggctcac aaggcccagg ctcaggcgga caacaaggac caggcggaca 781 aggcggctac ggaccaggcg ccggacaaca aggcccaggc agccaaggac caggcagcgg 841 cggccaacaa ggcccaggag gacaaggacc atacggccca agcgcagcag ccgcagcagc 901 cgcagcctac tagagggcgg atacggccca ggcgcaggcc aacaaggacc aggcagccaa 961 ggaccaggct caggcggaca acaaggccca ggcggacaag gcggctacgg cccaggcgcc 1021 ggacaacaag gaccaggctc acaaggacca ggctccggcg gacaacaagg accaggcggc 1081 caaggaccat acggaccaag cgcagcagcc gcagcagccg ccgca //