LOCUS       BBa_K1085028             985 bp    DNA     linear       06-Nov-2024
DEFINITION  RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 x2 
            SilkTail Strep-tag Stop-codon
ACCESSION   BBa_K1085028
VERSION     BBa_K1085028.1  
FEATURES             Location/Qualifiers
     RBS             1..6
                     /label=RBS
     CDS             17..226
                     /label=spidersilk subunit E1
     misc_feature    14..16
                     /label=starting codon (ATG)
     CDS             1..226
                     /label=BBa_K1085001
     CDS             235..444
                     /label=spidersilk subunit E2
     CDS             235..444
                     /label=BBa_K1085002
     CDS             453..662
                     /label=spidersilk subunit E2
     CDS             453..662
                     /label=BBa_K1085002
     misc_feature    959..982
                     /label=strep
     CDS             671..940
                     /label=spidersilk C-terminal domain
     misc_feature    983..985
                     /label=Stop codon (TGA)
     misc_feature    671..985
                     /label=BBa_K1085009
ORIGIN
        1 aggaggcata tccatgggcg gatacggacc aggagcaggc caacaaggcc caggctcaca
       61 aggcccaggc tcaggcggac aacaaggacc aggcggacaa ggcggctacg gaccaggcgc
      121 cggacaacaa ggcccaggca gccaaggacc aggcagcggc ggccaacaag gcccaggagg
      181 acaaggacca tacggcccaa gcgcagcagc cgcagcagcc gcagcctact agagggcgga
      241 tacggcccag gcgcaggcca acaaggacca ggcagccaag gaccaggctc aggcggacaa
      301 caaggcccag gcggacaagg cggctacggc ccaggcgccg gacaacaagg accaggctca
      361 caaggaccag gctccggcgg acaacaagga ccaggcggcc aaggaccata cggaccaagc
      421 gcagcagccg cagcagccgc cgcatactag agggcggata cggcccaggc gcaggccaac
      481 aaggaccagg cagccaagga ccaggctcag gcggacaaca aggcccaggc ggacaaggcg
      541 gctacggccc aggcgccgga caacaaggac caggctcaca aggaccaggc tccggcggac
      601 aacaaggacc aggcggccaa ggaccatacg gaccaagcgc agcagccgca gcagccgccg
      661 catactagag gccccagttg cctccgcagc cgcctcaagg ctgtcctccc ctcaagcctc
      721 ctcccgtgtt tcatccgccg tgtccactct cgtgtcctcc ggacctacga atcctgccgc
      781 cttatccaat gccatctcca gcgttgtatc acaagtttca gcctccaatc ctggactatc
      841 cggatgtgac gttctcgttc aagccctcct cgaactcgta tccgccctcg tacacatcct
      901 cggctcctcc tccattggac aaattaatta cgccgcctcc tccgcagcag catccgcatg
      961 gagccatccg cagtttgaaa aatga
//