BBa_K1085035
1
BBa_K1085035
EstA Strep-tag SpiderSilkSubunitE1
2013-10-03T11:00:00Z
2015-05-08T01:09:05Z
The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008) and are from the MaSp2 of the <i>Argiope aurantia</i>.
The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
This biobrick contains signal sequence EstA attached to the silk subunit E1 with an N-terminal strep-tag.
The Strep-tag, fused at the N-terminal, is used both as a binding tag to use to indicate its production and as a binding component to Streptavidin.
Subunit E1 codes for a spider silk protein that is optimized for maximal expression in <i>Bacillus subtilis</i> 168. The primary goal of the algorithm was to optimize the codon selection based on their availability scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tertiary goal was to minimize the number of restriction sites.
The amino acid sequence is based on the adapted MaSp2 sequences described in the paper of Brooks et. al. 2008.
false
false
_1395_
0
16111
9
Not in stock
false
The DNA sequence of subunit E1 was codon-optimized for Bacillus subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
false
Mirjam Groenewold
annotation2367749
1
starting codon (ATG)
range2367749
1
15
17
annotation2367750
1
estA-SRP
range2367750
1
18
107
annotation2367752
1
spider silk subunit E1
range2367752
1
156
365
annotation2367751
1
strep
range2367751
1
114
137
annotation2367748
1
RBS
range2367748
1
1
6
BBa_K1085035_sequence
1
aggaggaggatattatgaaatttgtaaaaagaaggatcattgcacttgtaacaattttgatgctgtctgttacatcgctgtttgcgttgcagccgtcagcaaaagccggatcctggagccatccgcagtttgaaaaatccgcagcagcatccgcaggcggatacggaccaggagcaggccaacaaggcccaggctcacaaggcccaggctcaggcggacaacaaggaccaggcggacaaggcggctacggaccaggcgccggacaacaaggcccaggcagccaaggaccaggcagcggcggccaacaaggcccaggaggacaaggaccatacggcccaagcgcagcagccgcagcagccgcagcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z