LOCUS BBa_K1085050 3604 bp DNA linear 23-Dec-2024 DEFINITION des knock out ACCESSION BBa_K1085050 VERSION BBa_K1085050.1 FEATURES Location/Qualifiers misc_feature 1..1072 /label=DesK up CDS 1240..2526 /label=Tet misc_feature 2675..3604 /label=DesK down ORIGIN 1 atccggcagc attaagtggg ttgatttgta aaaaagtctg gaagaaagag ccaagatatg 61 ctgtaatggt ataaaaccat tgatggaggg ttctatgtac gcctcaaaac tgaaaaaggg 121 agacgaaatc cggatcgtgt ctcccgcgac cagcatgtct atattatcaa atgaagcaaa 181 aatccaagca aaaacggcgt tagaacgctt aggttatcgg gtaaccatcg ctgaacacgc 241 aaatgaatgt aacgaatttg attcatcctc tatcgagtca agagtccatg atctgcacgc 301 tgcctttttt gatccaggcg tcaaagcgat tttaacgaca ctcggagggt ttaattcaaa 361 tcagctgctg cgttatcttg attacgagaa aatcaaacga catccaaaaa ttctgtgcgg 421 ttattccgat ataaccgctc tttgcaacgc tatttatcaa aaaacgggtc ttgttacgta 481 ttcaggtccc cacttctcaa catttgcaat gaaaaaagga ctcgattata cagaggagta 541 ttttctttct tgctgcgctt cagacgatcc gtttgaaatt cacccctcaa gtgagtggag 601 cgatgatcgc tggtttttgg atcaagaaaa caggcgtttt tatcctaata acggccctgt 661 tgtaatccaa gaaggttatg cggaaggtac tttaattggc ggaaacttat gcacgcttaa 721 tttgctgcag ggaacggagt attttcccga aacagaacat actattttat tgattgaaga 781 tgattatatg tcagacatcc atatgtttga ccgcgatctg caatcactca tccacctccc 841 cgctttttca catgtaaagg cgattttgat cggcagattt cagaaagcat caaacgtatc 901 aatagatctt gtaaaagcca tgatcgaaac aaaaaaagaa ttatccggca tcccgatcat 961 cgcaaatata aatgccggac atacctcgcc aattgccacg ttccctatag gaggaacatg 1021 caggattgaa gctatttcgg gtacatcacg aatatggatt gataaacatt aatcagcttg 1081 taaatttttt tacaagcttt tttagcgcaa tcggctatgc atgccgcacg agacatgaca 1141 aatgtcatat aggaggcatg atgtgtgcta ctacaaaaga cttctctcat tagcgtatac 1201 tgaaccgaga cacacaatga gaggatactt actgaattca tggttttgaa cgtctcatta 1261 cctgatattg caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc 1321 tttatgttaa ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc 1381 atcaaaaggt tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt 1441 gttggccatt ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca 1501 gctgcatttc cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg 1561 ggtaaagcat ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg 1621 attggtggaa tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata 1681 acaattatca ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt 1741 cattttgata tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt 1801 acaacatcat atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta 1861 aaacatatca ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct 1921 tttatgattg gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct 1981 atggttcctt atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta 2041 attattttcc ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt 2101 gatagaagag gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt 2161 ttaactgctt cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt 2221 gttttaggtg ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg 2281 aaacagcagg aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag 2341 ggaacaggta ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg 2401 ttacctatgg aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca 2461 ggaatcattg tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat 2521 ttctaaatcg ttaagggatc aactttgggg gatcctgata aaggaggtct tctaatatac 2581 tagaaggctt cctttttatt gttggagggt gcattctggc tttcacaatg ataaagttac 2641 aagtaagatg aaaagctgaa aatgaggtaa gatcatgatt aaaaatcatt ttacatttca 2701 aaaactaaac gggattacgc cctatatatg gacgatattt ttcatcctcc ccttctactt 2761 tatatggaaa tcatcatcta catttgttat tattgtcggc atcattttga cgcttttatt 2821 tttttcggta tatcgatttg cttttgtctc aaaaggctgg accatttatt tgtggggatt 2881 tttattaatt ggcatttcaa ccgcctccat tactctgttc agttatattt attttgcttt 2941 ctttattgct tattttattg gaaacattaa ggaacgcgtc ccttttcata ttttatatta 3001 tgtccattta ataagcgcgg ccgtcgcagc caatttcagt ctcgtattaa aaaaggaatt 3061 ctttctgaca caaattcctt ttgtcgttat taccctcatc agcgcaattt tattgccctt 3121 cagtataaaa agccgcaagg agcgcgaacg acttgaagaa aagctcgagg atgcaaatga 3181 acggattgca gaactggtaa aattagaaga acgtcagcga attgcccgcg atctccatga 3241 tacgcttggg caaaagcttt ctcttattgg tttaaaaagc gacttagcaa gaaaattgat 3301 atacaaagat cccgaacaag cagctcgtga actgaaaagt gttcagcaaa cagcgcgaac 3361 ttctttaaat gaagtaagaa aaatcgtttc ctctatgaag ggcatccggc tcaaggatga 3421 attgatcaac atcaaacaaa ttctcgaagc agctgacatt atgtttatct atgaagaaga 3481 gaaatggccc gagaatatct cattgctgaa tgaaaacatt ttgagcatgt gcttaaagga 3541 agctgtcaca aatgtcgtca aacacagcca ggctaaaact tgccgagttg acattcagca 3601 gctc //