LOCUS       BBa_K1086003             954 bp    DNA     linear       21-May-2024
DEFINITION  TorCad promoter + RFP
ACCESSION   BBa_K1086003
VERSION     BBa_K1086003.1  
FEATURES             Location/Qualifiers
     promoter        1..77
                     /label=BBa_K1086000
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..814
                     /label=mCherry
     misc_feature    455..455
                     /label=C->T (removing PstI site)
     misc_feature    104..817
                     /label=BBa_J06504
     stem_loop       837..880
                     /label=stem_loop
     misc_feature    826..905
                     /label=BBa_B0010
     terminator      826..905
                     /label=BBa_B0010
     stem_loop       921..940
                     /label=T7 TE
     misc_feature    947..947
                     /label=stop
     polyA_site      941..954
                     /label=polya
     misc_feature    914..954
                     /label=BBa_B0012
     terminator      914..954
                     /label=BBa_B0012
     misc_feature    86..954
                     /label=BBa_J06702
ORIGIN
        1 attctgttca tatctgttca tattccgttc atcctgacca gtgccgctgt tcatatttgc
       61 tcattaagat cgcttcatac tagagaaaga ggagaaatac tagatggtga gcaagggcga
      121 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      181 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      241 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      301 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      361 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      421 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa
      481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      541 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      601 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      661 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      721 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      781 ctccaccggc ggcatggacg agctgtacaa gtaataatac tagagccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//