LOCUS       BBa_K1087015             954 bp    DNA     linear       30-Jun-2024
DEFINITION  Ptet-lock 3d-mRFP1-teminator
ACCESSION   BBa_K1087015
VERSION     BBa_K1087015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..105
                     /label=BBa_J23032
     promoter        1..105
                     /label=BBa_K1087004
     CDS             112..786
                     /label=mrfp1
     misc_feature    793..817
                     /label=Help:Barcodes
     CDS             112..792
                     /label=BBa_E1010
     stem_loop       837..880
                     /label=stem_loop
     misc_feature    826..905
                     /label=BBa_B0010
     terminator      826..905
                     /label=BBa_B0010
     stem_loop       921..940
                     /label=T7 TE
     misc_feature    947..947
                     /label=stop
     polyA_site      941..954
                     /label=polya
     misc_feature    914..954
                     /label=BBa_B0012
     terminator      914..954
                     /label=BBa_B0012
     misc_feature    112..954
                     /label=BBa_J04650
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg cttttgggta agacagaaga ggagatacta gatggcttcc
      121 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      181 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      241 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      301 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      361 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      421 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      481 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      541 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      601 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      661 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
      721 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      781 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//