LOCUS BBa_K1087017 2030 bp DNA linear 26-Nov-2024 DEFINITION RBS +cre-ssrA(LAA)tag +RBS+GFP-ssrA +teminator ACCESSION BBa_K1087017 VERSION BBa_K1087017.1 FEATURES Location/Qualifiers misc_feature 1..1108 /label=BBa_K1087000 misc_feature 1121..1124 /label=conserved RBS 1117..1128 /label=BBa_B0034 misc_feature 1135..1893 /label=BBa_E0044 stem_loop 1913..1956 /label=stem_loop misc_feature 1902..1981 /label=BBa_B0010 terminator 1902..1981 /label=BBa_B0010 stem_loop 1997..2016 /label=T7 TE misc_feature 2023..2023 /label=stop polyA_site 2017..2030 /label=polya misc_feature 1990..2030 /label=BBa_B0012 terminator 1990..2030 /label=BBa_B0012 terminator 1902..2030 /label=BBa_B0015 misc_feature 1135..2030 /label=BBa_K1087011 misc_feature 1117..2030 /label=BBa_K1087012 ORIGIN 1 gggctagcta ctagagatta aagaggagaa atactagatg tccaatttac tgaccgtaca 61 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat 121 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt 181 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc 241 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat 301 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag 361 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg 421 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact 481 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta 541 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac 601 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg 661 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc 721 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc 781 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac 841 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg 901 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga 961 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct 1021 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgatcgac ctgccgcaaa 1081 cgacgaaaac tacgctttag cagcttaata ctagagaaag aggagaaata ctagatgcgt 1141 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 1201 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 1261 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 1321 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 1381 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 1441 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 1501 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 1561 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 1621 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 1681 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 1741 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 1801 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca 1861 aacgacgaaa actacgctgc tgctgtttaa taatactaga gccaggcatc aaataaaacg 1921 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1981 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //