LOCUS       BBa_K1087021            1360 bp    DNA     linear       14-Jun-2024
DEFINITION  RiboKey+Lock+mRFP1
ACCESSION   BBa_K1087021
VERSION     BBa_K1087021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..156
                     /label=BBa_J23008
     misc_feature    165..261
                     /label=BBa_J23009
     stem_loop       281..324
                     /label=stem_loop
     misc_feature    270..349
                     /label=BBa_B0010
     terminator      270..349
                     /label=BBa_B0010
     stem_loop       365..384
                     /label=T7 TE
     misc_feature    391..391
                     /label=stop
     polyA_site      385..398
                     /label=polya
     misc_feature    358..398
                     /label=BBa_B0012
     terminator      358..398
                     /label=BBa_B0012
     misc_feature    1..398
                     /label=BBa_K145215
     misc_feature    407..425
                     /label=TetR 1
     misc_feature    407..460
                     /label=BBa_R0040
     promoter        426..431
                     /label=-35
     misc_feature    432..450
                     /label=TetR 2
     promoter        449..454
                     /label=-10
     promoter        407..460
                     /label=BBa_R0040
     misc_feature    469..511
                     /label=BBa_J23032
     promoter        407..511
                     /label=BBa_K1087004
     CDS             518..1192
                     /label=mrfp1
     misc_feature    1199..1223
                     /label=Help:Barcodes
     CDS             518..1198
                     /label=BBa_E1010
     stem_loop       1243..1286
                     /label=stem_loop
     misc_feature    1232..1311
                     /label=BBa_B0010
     terminator      1232..1311
                     /label=BBa_B0010
     stem_loop       1327..1346
                     /label=T7 TE
     misc_feature    1353..1353
                     /label=stop
     polyA_site      1347..1360
                     /label=polya
     misc_feature    1320..1360
                     /label=BBa_B0012
     terminator      1320..1360
                     /label=BBa_B0012
     misc_feature    518..1360
                     /label=BBa_J04650
     misc_feature    407..1360
                     /label=BBa_K1087015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacccaaaa gcaagaggtg attctagttg gtggttaatg aaaattaact tacttactag
      121 aaatatctct aaaaagccag attattaatc cggctttact agagcgggcg ggcgggcggg
      181 cgggatccat aaaaaaaaaa cccaaaagca agaggtgatt ctagttaaaa aaaaaaagct
      241 tcccgcccgc ccgcccgccc gtactagagc caggcatcaa ataaaacgaa aggctcagtc
      301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      361 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat
      421 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa ctagaatcac
      481 ctcttgcttt tgggtaagac agaagaggag atactagatg gcttcctccg aagacgttat
      541 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      601 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      661 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      721 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      781 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      841 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      901 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      961 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1021 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1081 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1141 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     1201 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     1261 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1321 cacactggct caccttcggg tgggcctttc tgcgtttata
//