##gff-version 3
##sequence-region BBa_K1087021 1 1360
BBa_K1087021	.	engineered_region	1	398	.	+	0	ID=BBa_K145215;Name=BBa_K145215
BBa_K1087021	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K145215
BBa_K1087021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145215
BBa_K1087021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1087021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1087021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1087021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1087021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1087021	.	terminator	270	349	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145215
BBa_K1087021	.	engineered_region	270	349	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1087021	.	stem_loop	281	324	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1087021	.	terminator	358	398	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145215
BBa_K1087021	.	engineered_region	358	398	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1087021	.	stop_codon	391	391	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1087021	.	stem_loop	365	384	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1087021	.	polyA_site	385	398	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1087021	.	mature_transcript_region	165	261	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K145215
BBa_K1087021	.	engineered_region	407	1360	.	+	0	ID=BBa_K1087015;Name=BBa_K1087015
BBa_K1087021	.	promoter	407	511	.	+	0	ID=BBa_K1087004;Name=BBa_K1087004;Parent=BBa_K1087015
BBa_K1087021	.	promoter	407	460	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1087004
BBa_K1087021	.	promoter	426	431	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1087021	.	non_covalent_binding_site	432	450	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1087021	.	engineered_region	407	460	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1087021	.	promoter	449	454	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1087021	.	non_covalent_binding_site	407	425	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1087021	.	mature_transcript_region	469	511	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K1087004
BBa_K1087021	.	engineered_region	518	1360	.	+	0	ID=BBa_J04650;Name=BBa_J04650;Parent=BBa_K1087015
BBa_K1087021	.	terminator	1232	1311	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_K1087021	.	engineered_region	1232	1311	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1087021	.	stem_loop	1243	1286	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1087021	.	terminator	1320	1360	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_K1087021	.	engineered_region	1320	1360	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1087021	.	stop_codon	1353	1353	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1087021	.	stem_loop	1327	1346	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1087021	.	polyA_site	1347	1360	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1087021	.	CDS	518	1198	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_K1087021	.	engineered_tag	1199	1223	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1087021	.	CDS	518	1192	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K1087021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggt
gattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaa
gcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactag
aatcacctcttgcttttgggtaagacagaagaggagatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata