BBa_K1087021
type: ComponentDefinition
persistent identity: BBa K1087021
display id: BBa_K1087021
version: 1
was generated by: iGEM to SBOL conversion
description: RiboKey+Lock+mRFP1
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: 3A assembly
owned by: james
owned by: myers
mutable description: This part was constructed by assemblying BBa_K145215 and BBa_K1087015 to test the function of riboregulator.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K1087015
component: BBa_K145215
sequence annotation: BBa_K145215
sequence annotation: BBa_K1087015
sequence: https://synbiohub.org/public/igem/BBa_K1087021_sequence/1

BBa_K1087015
type: Component
persistent identity: component2364518
display id: component2364518
version: 1
top level: BBa_K1087021
definition: BBa_K1087015
access: public

BBa_K145215
type: Component
persistent identity: component2364500
display id: component2364500
version: 1
top level: BBa_K1087021
definition: BBa_K145215
access: public

BBa_K145215
type: SequenceAnnotation
persistent identity: annotation2364500
display id: annotation2364500
version: 1
top level: BBa_K1087021
location: https://synbiohub.org/public/igem/BBa_K1087021/annotation2364500/range2364500/1
component: BBa_K145215

https://synbiohub.org/public/igem/BBa_K1087021/annotation2364500/range2364500/1
type: Range
persistent identity: range2364500
display id: range2364500
version: 1
top level: BBa_K1087021
direction: direction/reverse
start: 1
end: 398
orientation: inline

BBa_K1087015
type: SequenceAnnotation
persistent identity: annotation2364518
display id: annotation2364518
version: 1
top level: BBa_K1087021
location: https://synbiohub.org/public/igem/BBa_K1087021/annotation2364518/range2364518/1
component: BBa_K1087015

https://synbiohub.org/public/igem/BBa_K1087021/annotation2364518/range2364518/1
type: Range
persistent identity: range2364518
display id: range2364518
version: 1
top level: BBa_K1087021
direction: direction/reverse
start: 407
end: 1360
orientation: inline

BBa_K145215
type: ComponentDefinition
persistent identity: BBa K145215
display id: BBa_K145215
version: 1
was generated by: iGEM to SBOL conversion
description: FILTER Key (TetR promoter + key)
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: other parts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We did a lot of modeling on this device. The most efficient Berkeley 2006 key and lock have been used. The RiboKy might be required in higher copy number although modeling doesn't predict this.
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_J23009
component: BBa_J23008
sequence annotation: BBa_R0040
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145215_sequence/1

BBa_R0040
type: Component
persistent identity: component1974063
display id: component1974063
version: 1
top level: BBa_K145215
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1974071
display id: component1974071
version: 1
top level: BBa_K145215
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1974069
display id: component1974069
version: 1
top level: BBa_K145215
definition: BBa_B0010
access: public

BBa_J23009
type: Component
persistent identity: component1974068
display id: component1974068
version: 1
top level: BBa_K145215
definition: BBa_J23009
access: public

BBa_J23008
type: Component
persistent identity: component1974067
display id: component1974067
version: 1
top level: BBa_K145215
definition: BBa_J23008
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1974063
display id: annotation1974063
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
type: Range
persistent identity: range1974063
display id: range1974063
version: 1
top level: BBa_K145215
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation1974067
display id: annotation1974067
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
type: Range
persistent identity: range1974067
display id: range1974067
version: 1
top level: BBa_K145215
direction: direction/reverse
start: 63
end: 156
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation1974068
display id: annotation1974068
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
type: Range
persistent identity: range1974068
display id: range1974068
version: 1
top level: BBa_K145215
direction: direction/reverse
start: 165
end: 261
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974069
display id: annotation1974069
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
type: Range
persistent identity: range1974069
display id: range1974069
version: 1
top level: BBa_K145215
direction: direction/forward
start: 270
end: 349
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974071
display id: annotation1974071
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
type: Range
persistent identity: range1974071
display id: range1974071
version: 1
top level: BBa_K145215
direction: direction/forward
start: 358
end: 398
orientation: inline

BBa_K1087015
type: ComponentDefinition
persistent identity: BBa K1087015
display id: BBa_K1087015
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet-lock 3d-mRFP1-teminator
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: No
owned by: james
owned by: myers
mutable description: This part was designed to test the function of the riboregulator Ptet-lock 3d. The expression of the reporter should be low without the key BBa_K145215 and high when cotransfected with the key.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J04650
component: BBa_K1087004
sequence annotation: BBa_K1087004
sequence annotation: BBa_J04650
sequence: https://synbiohub.org/public/igem/BBa_K1087015_sequence/1

BBa_J04650
type: Component
persistent identity: component2340416
display id: component2340416
version: 1
top level: BBa_K1087015
definition: mRFP1
access: public

BBa_K1087004
type: Component
persistent identity: component2340406
display id: component2340406
version: 1
top level: BBa_K1087015
definition: BBa_K1087004
access: public

BBa_K1087004
type: SequenceAnnotation
persistent identity: annotation2340406
display id: annotation2340406
version: 1
top level: BBa_K1087015
location: https://synbiohub.org/public/igem/BBa_K1087015/annotation2340406/range2340406/1
component: BBa_K1087004

https://synbiohub.org/public/igem/BBa_K1087015/annotation2340406/range2340406/1
type: Range
persistent identity: range2340406
display id: range2340406
version: 1
top level: BBa_K1087015
direction: direction/reverse
start: 1
end: 105
orientation: inline

BBa_J04650
type: SequenceAnnotation
persistent identity: annotation2340416
display id: annotation2340416
version: 1
top level: BBa_K1087015
location: https://synbiohub.org/public/igem/BBa_K1087015/annotation2340416/range2340416/1
component: BBa_J04650

https://synbiohub.org/public/igem/BBa_K1087015/annotation2340416/range2340416/1
type: Range
persistent identity: range2340416
display id: range2340416
version: 1
top level: BBa_K1087015
direction: direction/reverse
start: 112
end: 954
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa J04650
display id: BBa_J04650
version: 1
was generated by: iGEM to SBOL conversion
description: E1010.B0015
created: 2005-08-28T11:00:00Z
modified: 2015-08-31T04:08:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part results from ligating E1010 with B0015 in the manner described on this website.
discontinued: false
dominant: false
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04650_sequence/1

BBa_E1010
type: Component
persistent identity: component1676935
display id: component1676935
version: 1
top level: mRFP1
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1676952
display id: component1676952
version: 1
top level: mRFP1
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1676942
display id: component1676942
version: 1
top level: mRFP1
definition: BBa_B0010
access: public

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1676935
display id: annotation1676935
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676935/range1676935/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04650/annotation1676935/range1676935/1
type: Range
persistent identity: range1676935
display id: range1676935
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 681
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1676942
display id: annotation1676942
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676942/range1676942/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04650/annotation1676942/range1676942/1
type: Range
persistent identity: range1676942
display id: range1676942
version: 1
top level: mRFP1
direction: direction/forward
start: 715
end: 794
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1676952
display id: annotation1676952
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676952/range1676952/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04650/annotation1676952/range1676952/1
type: Range
persistent identity: range1676952
display id: range1676952
version: 1
top level: mRFP1
direction: direction/forward
start: 803
end: 843
orientation: inline

BBa_K1087004
type: ComponentDefinition
persistent identity: BBa K1087004
display id: BBa_K1087004
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet-Riboregulator(lock 3d)
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: 3A assembly
owned by: james
owned by: myers
mutable description: This is riboregulator that can express downstream gene with a key BBa_K145215, while "lock" it without the key.We assemblied this part as an alternative to BBa_I714070 which has a poor quality and cannot be transformed from the kit plate.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_J23032
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J23032
sequence: https://synbiohub.org/public/igem/BBa_K1087004_sequence/1

BBa_J23032
type: Component
persistent identity: component2339160
display id: component2339160
version: 1
top level: BBa_K1087004
definition: BBa_J23032
access: public

BBa_R0040
type: Component
persistent identity: component2339155
display id: component2339155
version: 1
top level: BBa_K1087004
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2339155
display id: annotation2339155
version: 1
top level: BBa_K1087004
location: https://synbiohub.org/public/igem/BBa_K1087004/annotation2339155/range2339155/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1087004/annotation2339155/range2339155/1
type: Range
persistent identity: range2339155
display id: range2339155
version: 1
top level: BBa_K1087004
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2339160
display id: annotation2339160
version: 1
top level: BBa_K1087004
location: https://synbiohub.org/public/igem/BBa_K1087004/annotation2339160/range2339160/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K1087004/annotation2339160/range2339160/1
type: Range
persistent identity: range2339160
display id: range2339160
version: 1
top level: BBa_K1087004
direction: direction/reverse
start: 63
end: 105
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

https://synbiohub.org/public/igem/BBa_K1087021_sequence/1
type: Sequence
persistent identity: BBa K1087021 sequence
display id: BBa_K1087021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145215_sequence/1
type: Sequence
persistent identity: BBa K145215 sequence
display id: BBa_K145215_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087015_sequence/1
type: Sequence
persistent identity: BBa K1087015 sequence
display id: BBa_K1087015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04650_sequence/1
type: Sequence
persistent identity: BBa J04650 sequence
display id: BBa_J04650_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087004_sequence/1
type: Sequence
persistent identity: BBa K1087004 sequence
display id: BBa_K1087004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z