##gff-version 3
##sequence-region BBa_K1087022 1 1463
BBa_K1087022	.	engineered_region	503	1463	.	+	0	ID=BBa_K1087018;Name=BBa_K1087018
BBa_K1087022	.	engineered_region	603	1463	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K1087018
BBa_K1087022	.	ribosome_entry_site	603	614	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1087022	.	conserved_region	607	610	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1087022	.	terminator	1423	1463	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1087022	.	polyA_site	1450	1463	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1087022	.	engineered_region	1423	1463	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1087022	.	stem_loop	1430	1449	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1087022	.	stop_codon	1456	1456	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1087022	.	terminator	1335	1414	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1087022	.	stem_loop	1346	1389	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1087022	.	engineered_region	1335	1414	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1087022	.	CDS	621	1301	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1087022	.	CDS	621	1295	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1087022	.	engineered_tag	1302	1326	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1087022	.	promoter	503	594	.	+	0	ID=BBa_I746361;Name=BBa_I746361;Parent=BBa_K1087018
BBa_K1087022	.	promoter	503	594	.	+	0	ID=annotation1943784;Name=PO;Parent=BBa_I746361
BBa_K1087022	.	promoter	1	494	.	+	0	ID=BBa_K1087007;Name=BBa_K1087007
BBa_K1087022	.	engineered_region	112	494	.	+	0	ID=BBa_K1087006;Name=BBa_K1087006;Parent=BBa_K1087007
BBa_K1087022	.	promoter	1	105	.	+	0	ID=BBa_K1087004;Name=BBa_K1087004;Parent=BBa_K1087007
BBa_K1087022	.	mature_transcript_region	63	105	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K1087004
BBa_K1087022	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1087004
BBa_K1087022	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1087022	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1087022	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1087022	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1087022	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K1087022
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctt
gcttttgggtaagacagaagaggagatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccag
ccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcat
gagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtcc
gaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgg
gactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagaaagaggagaaatactagatggcttcctcc
gaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaag
gtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgc
ttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctg
aaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccagg
actcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttat
gcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaa
atgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagc
tgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacga
acgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata