BBa_K1087022
type: ComponentDefinition
persistent identity: BBa K1087022
display id: BBa_K1087022
version: 1
was generated by: iGEM to SBOL conversion
description: Lock+phiR73 activator+PO promoter+mRFP1
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: 3A assembly
owned by: james
owned by: myers
mutable description: This part was constructed by assemblying BBa_K1087007 and BBa_K1087018 to test the function of ribolock and phiR73
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1087018
component: BBa_K1087007
sequence annotation: BBa_K1087007
sequence annotation: BBa_K1087018
sequence: https://synbiohub.org/public/igem/BBa_K1087022_sequence/1

BBa_K1087018
type: Component
persistent identity: component2364542
display id: component2364542
version: 1
top level: BBa_K1087022
definition: BBa_K1087018
access: public

BBa_K1087007
type: Component
persistent identity: component2364527
display id: component2364527
version: 1
top level: BBa_K1087022
definition: BBa_K1087007
access: public

BBa_K1087007
type: SequenceAnnotation
persistent identity: annotation2364527
display id: annotation2364527
version: 1
top level: BBa_K1087022
location: https://synbiohub.org/public/igem/BBa_K1087022/annotation2364527/range2364527/1
component: BBa_K1087007

https://synbiohub.org/public/igem/BBa_K1087022/annotation2364527/range2364527/1
type: Range
persistent identity: range2364527
display id: range2364527
version: 1
top level: BBa_K1087022
direction: direction/reverse
start: 1
end: 494
orientation: inline

BBa_K1087018
type: SequenceAnnotation
persistent identity: annotation2364542
display id: annotation2364542
version: 1
top level: BBa_K1087022
location: https://synbiohub.org/public/igem/BBa_K1087022/annotation2364542/range2364542/1
component: BBa_K1087018

https://synbiohub.org/public/igem/BBa_K1087022/annotation2364542/range2364542/1
type: Range
persistent identity: range2364542
display id: range2364542
version: 1
top level: BBa_K1087022
direction: direction/reverse
start: 503
end: 1463
orientation: inline

BBa_K1087007
type: ComponentDefinition
persistent identity: BBa K1087007
display id: BBa_K1087007
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet-lock 3d-PhiR73 activator-B0015
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: 3A assembly
owned by: james
owned by: myers
mutable description: In this part,the expression of PhiR73 activator is controlled by the riboregulator lock3d. When this part is cotransfected with the key BBa_K145215, the "lock" can be opened and the PhiR73 activator can be expressed to induce its promoters such as BBa_I746361.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1087006
component: BBa_K1087004
sequence annotation: BBa_K1087004
sequence annotation: BBa_K1087006
sequence: https://synbiohub.org/public/igem/BBa_K1087007_sequence/1

BBa_K1087006
type: Component
persistent identity: component2339180
display id: component2339180
version: 1
top level: BBa_K1087007
definition: BBa_K1087006
access: public

BBa_K1087004
type: Component
persistent identity: component2339179
display id: component2339179
version: 1
top level: BBa_K1087007
definition: BBa_K1087004
access: public

BBa_K1087004
type: SequenceAnnotation
persistent identity: annotation2339179
display id: annotation2339179
version: 1
top level: BBa_K1087007
location: https://synbiohub.org/public/igem/BBa_K1087007/annotation2339179/range2339179/1
component: BBa_K1087004

https://synbiohub.org/public/igem/BBa_K1087007/annotation2339179/range2339179/1
type: Range
persistent identity: range2339179
display id: range2339179
version: 1
top level: BBa_K1087007
direction: direction/reverse
start: 1
end: 105
orientation: inline

BBa_K1087006
type: SequenceAnnotation
persistent identity: annotation2339180
display id: annotation2339180
version: 1
top level: BBa_K1087007
location: https://synbiohub.org/public/igem/BBa_K1087007/annotation2339180/range2339180/1
component: BBa_K1087006

https://synbiohub.org/public/igem/BBa_K1087007/annotation2339180/range2339180/1
type: Range
persistent identity: range2339180
display id: range2339180
version: 1
top level: BBa_K1087007
direction: direction/reverse
start: 112
end: 494
orientation: inline

BBa_K1087018
type: ComponentDefinition
persistent identity: BBa K1087018
display id: BBa_K1087018
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter+mRFP1
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: NO
owned by: james
owned by: myers
mutable description: This is a RFP reporter controlled by PO promoter from phage.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_I746361
sequence annotation: BBa_I746361
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1087018_sequence/1

BBa_I13507
type: Component
persistent identity: component2341031
display id: component2341031
version: 1
top level: BBa_K1087018
definition: BBa_I13507
access: public

BBa_I746361
type: Component
persistent identity: component2341019
display id: component2341019
version: 1
top level: BBa_K1087018
definition: BBa_I746361
access: public

BBa_I746361
type: SequenceAnnotation
persistent identity: annotation2341019
display id: annotation2341019
version: 1
top level: BBa_K1087018
location: https://synbiohub.org/public/igem/BBa_K1087018/annotation2341019/range2341019/1
component: BBa_I746361

https://synbiohub.org/public/igem/BBa_K1087018/annotation2341019/range2341019/1
type: Range
persistent identity: range2341019
display id: range2341019
version: 1
top level: BBa_K1087018
direction: direction/reverse
start: 1
end: 92
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2341031
display id: annotation2341031
version: 1
top level: BBa_K1087018
location: https://synbiohub.org/public/igem/BBa_K1087018/annotation2341031/range2341031/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1087018/annotation2341031/range2341031/1
type: Range
persistent identity: range2341031
display id: range2341031
version: 1
top level: BBa_K1087018
direction: direction/reverse
start: 101
end: 961
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_K1087004
type: ComponentDefinition
persistent identity: BBa K1087004
display id: BBa_K1087004
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet-Riboregulator(lock 3d)
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: 3A assembly
owned by: james
owned by: myers
mutable description: This is riboregulator that can express downstream gene with a key BBa_K145215, while "lock" it without the key.We assemblied this part as an alternative to BBa_I714070 which has a poor quality and cannot be transformed from the kit plate.
discontinued: false
dominant: false
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_J23032
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J23032
sequence: https://synbiohub.org/public/igem/BBa_K1087004_sequence/1

BBa_J23032
type: Component
persistent identity: component2339160
display id: component2339160
version: 1
top level: BBa_K1087004
definition: BBa_J23032
access: public

BBa_R0040
type: Component
persistent identity: component2339155
display id: component2339155
version: 1
top level: BBa_K1087004
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2339155
display id: annotation2339155
version: 1
top level: BBa_K1087004
location: https://synbiohub.org/public/igem/BBa_K1087004/annotation2339155/range2339155/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1087004/annotation2339155/range2339155/1
type: Range
persistent identity: range2339155
display id: range2339155
version: 1
top level: BBa_K1087004
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2339160
display id: annotation2339160
version: 1
top level: BBa_K1087004
location: https://synbiohub.org/public/igem/BBa_K1087004/annotation2339160/range2339160/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K1087004/annotation2339160/range2339160/1
type: Range
persistent identity: range2339160
display id: range2339160
version: 1
top level: BBa_K1087004
direction: direction/reverse
start: 63
end: 105
orientation: inline

BBa_K1087006
type: ComponentDefinition
persistent identity: BBa K1087006
display id: BBa_K1087006
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage without RBS but with B0015
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: PCRed from BBa_K1087005
owned by: james
owned by: myers
mutable description: This part was PCRed from BBa_K1087005 to get rid of the RBS, so one can use this part with a riboregulator such as BBa_K1087004.
discontinued: false
dominant: false
experience: experience/None
group u list: _1397_
m user id: 0
owner id: 12187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No
star: false
creator: Tong Xu
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1087006_sequence/1

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: iGEM to SBOL conversion
description: PO promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
top level: BBa_I746361
direction: direction/forward
start: 1
end: 92
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

https://synbiohub.org/public/igem/BBa_K1087022_sequence/1
type: Sequence
persistent identity: BBa K1087022 sequence
display id: BBa_K1087022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087007_sequence/1
type: Sequence
persistent identity: BBa K1087007 sequence
display id: BBa_K1087007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087018_sequence/1
type: Sequence
persistent identity: BBa K1087018 sequence
display id: BBa_K1087018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087004_sequence/1
type: Sequence
persistent identity: BBa K1087004 sequence
display id: BBa_K1087004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1087006_sequence/1
type: Sequence
persistent identity: BBa K1087006 sequence
display id: BBa_K1087006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746361_sequence/1
type: Sequence
persistent identity: BBa I746361 sequence
display id: BBa_I746361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z