LOCUS       BBa_K1087023            1869 bp    DNA     linear       01-Jun-2024
DEFINITION  Ribokey+Lock+phiR73+PO promoter+mRFP1
ACCESSION   BBa_K1087023
VERSION     BBa_K1087023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..156
                     /label=BBa_J23008
     misc_feature    165..261
                     /label=BBa_J23009
     stem_loop       281..324
                     /label=stem_loop
     misc_feature    270..349
                     /label=BBa_B0010
     terminator      270..349
                     /label=BBa_B0010
     stem_loop       365..384
                     /label=T7 TE
     misc_feature    391..391
                     /label=stop
     polyA_site      385..398
                     /label=polya
     misc_feature    358..398
                     /label=BBa_B0012
     terminator      358..398
                     /label=BBa_B0012
     misc_feature    1..398
                     /label=BBa_K145215
     misc_feature    407..425
                     /label=TetR 1
     misc_feature    407..460
                     /label=BBa_R0040
     promoter        426..431
                     /label=-35
     misc_feature    432..450
                     /label=TetR 2
     promoter        449..454
                     /label=-10
     promoter        407..460
                     /label=BBa_R0040
     misc_feature    469..511
                     /label=BBa_J23032
     promoter        407..511
                     /label=BBa_K1087004
     misc_feature    518..900
                     /label=BBa_K1087006
     promoter        407..900
                     /label=BBa_K1087007
     promoter        909..1000
                     /label=PO
     promoter        909..1000
                     /label=BBa_I746361
     misc_feature    1013..1016
                     /label=conserved
     RBS             1009..1020
                     /label=BBa_B0034
     CDS             1027..1701
                     /label=mrfp1
     misc_feature    1708..1732
                     /label=Help:Barcodes
     CDS             1027..1707
                     /label=BBa_E1010
     stem_loop       1752..1795
                     /label=stem_loop
     misc_feature    1741..1820
                     /label=BBa_B0010
     terminator      1741..1820
                     /label=BBa_B0010
     stem_loop       1836..1855
                     /label=T7 TE
     misc_feature    1862..1862
                     /label=stop
     polyA_site      1856..1869
                     /label=polya
     misc_feature    1829..1869
                     /label=BBa_B0012
     terminator      1829..1869
                     /label=BBa_B0012
     misc_feature    1009..1869
                     /label=BBa_I13507
     misc_feature    909..1869
                     /label=BBa_K1087018
     misc_feature    407..1869
                     /label=BBa_K1087022
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacccaaaa gcaagaggtg attctagttg gtggttaatg aaaattaact tacttactag
      121 aaatatctct aaaaagccag attattaatc cggctttact agagcgggcg ggcgggcggg
      181 cgggatccat aaaaaaaaaa cccaaaagca agaggtgatt ctagttaaaa aaaaaaagct
      241 tcccgcccgc ccgcccgccc gtactagagc caggcatcaa ataaaacgaa aggctcagtc
      301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      361 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat
      421 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa ctagaatcac
      481 ctcttgcttt tgggtaagac agaagaggag atactagatg atgcgctgcc ctttctgtcg
      541 tcattcagcg catacccgca ccagccggta tgtgagtgac aatgtcaaag aaagttatct
      601 ccagtgccag aatatttact gttcggcgac atttaaaacg catgagtcaa tttgtgccgt
      661 gattcgttct ccggtcacgg aggaaaaacc agcaccggca agcacagcac cggctgttgt
      721 ccgaaaagtt aaaggctgtt acagctcacc attcaaccat taatactaga gccaggcatc
      781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      841 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      901 tactagagcg cgccccgcga ttcgctaagg tgctgttgtg tcagtgataa gccatccggg
      961 actgatggcg gaggatgcgc atcgtcggga aactgatgcc tactagagaa agaggagaaa
     1021 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     1081 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     1141 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1201 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1261 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1321 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1381 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     1441 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     1501 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     1561 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     1621 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     1681 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     1741 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1801 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1861 gcgtttata
//