BBa_K1088010
type: ComponentDefinition
persistent identity: BBa K1088010
display id: BBa_K1088010
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: With the exception of the artificial terminator and the GFP part from BBa_K1088007 (GFP = BBa_E0040), everything derives from E. coli K-12 MG1655
owned by: james
owned by: myers
mutable description: This is the same part as BBa_K1088007, with the exception of LacI under the control of a constitutive promoter to ensure regulation of the Lac Promoter This part consists of 5 subparts: A constitutive promoter: BBa_J23106 A strong RBS: BBa_B0030 The LacI repressor protein, regulating the lac promoter: BBa_C0012 An artificial terminator: BBa_B1002 and the rest is similar to BBa_K1088007
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The terminator aswell as the RBS and promoter was part of the reverse primer and forward primer for the LacI part, respectively. However we did insert a scar sequence between the RBS and startcodon to improve efficiency. There is also scar sites between the terminator (BBa_10002) and the rest of this composite part (BBa_K1088007). In the BBa_K1088007 part there is specific sites for scars aswell. Go to that parts page to investigate further.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K1088007
component: BBa_K1088019
sequence annotation: BBa_K1088019
sequence annotation: BBa_K1088007
sequence: https://synbiohub.org/public/igem/BBa_K1088010_sequence/1

BBa_K1088007
type: Component
persistent identity: component2351710
display id: component2351710
version: 1
top level: BBa_K1088010
definition: BBa_K1088007
access: public

BBa_K1088019
type: Component
persistent identity: component2351693
display id: component2351693
version: 1
top level: BBa_K1088010
definition: BBa_K1088019
access: public

BBa_K1088019
type: SequenceAnnotation
persistent identity: annotation2351693
display id: annotation2351693
version: 1
top level: BBa_K1088010
location: https://synbiohub.org/public/igem/BBa_K1088010/annotation2351693/range2351693/1
component: BBa_K1088019

https://synbiohub.org/public/igem/BBa_K1088010/annotation2351693/range2351693/1
type: Range
persistent identity: range2351693
display id: range2351693
version: 1
top level: BBa_K1088010
direction: direction/reverse
start: 1
end: 1173
orientation: inline

BBa_K1088007
type: SequenceAnnotation
persistent identity: annotation2351710
display id: annotation2351710
version: 1
top level: BBa_K1088010
location: https://synbiohub.org/public/igem/BBa_K1088010/annotation2351710/range2351710/1
component: BBa_K1088007

https://synbiohub.org/public/igem/BBa_K1088010/annotation2351710/range2351710/1
type: Range
persistent identity: range2351710
display id: range2351710
version: 1
top level: BBa_K1088010
direction: direction/reverse
start: 1182
end: 4013
orientation: inline

BBa_K1088007
type: ComponentDefinition
persistent identity: BBa K1088007
display id: BBa_K1088007
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: The subparts of this composite part is derived from E. coli K-12 MG1655. The GFP derives from Aequorea victoria.
owned by: james
owned by: myers
mutable description: This composite part consists of ? subparts: A Lac promoter: BBa_R0010 A strong RBS: BBa_J15001 The Dxs protein from E. coli: BBa_K118000 A 10 aa protein linker: BBa_K105012 A GFP reporter protein: BBa_E0040
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used USER cloning to assemble this part and therefore there are no scar sites. However we did insert a scarsite between the RBS and the start codon aswell as between the promoter and RBS, for optimal expression of our protein.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_R0010
component: BBa_K105012
component: BBa_K118000
component: BBa_J15001
component: BBa_K1088021
sequence annotation: BBa_R0010
sequence annotation: BBa_K1088021
sequence annotation: BBa_J15001
sequence annotation: BBa_K118000
sequence annotation: BBa_K105012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1088007_sequence/1

BBa_E0040
type: Component
persistent identity: component2367848
display id: component2367848
version: 1
top level: BBa_K1088007
definition: GFP
access: public

BBa_R0010
type: Component
persistent identity: component2367833
display id: component2367833
version: 1
top level: BBa_K1088007
definition: LacI
access: public

BBa_K105012
type: Component
persistent identity: component2367846
display id: component2367846
version: 1
top level: BBa_K1088007
definition: BBa_K105012
access: public

BBa_K118000
type: Component
persistent identity: component2367845
display id: component2367845
version: 1
top level: BBa_K1088007
definition: dxs
access: public

BBa_J15001
type: Component
persistent identity: component2367843
display id: component2367843
version: 1
top level: BBa_K1088007
definition: BBa_J15001
access: public

BBa_K1088021
type: Component
persistent identity: component2367840
display id: component2367840
version: 1
top level: BBa_K1088007
definition: BBa_K1088021
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2367833
display id: annotation2367833
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367833/range2367833/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367833/range2367833/1
type: Range
persistent identity: range2367833
display id: range2367833
version: 1
top level: BBa_K1088007
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1088021
type: SequenceAnnotation
persistent identity: annotation2367840
display id: annotation2367840
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367840/range2367840/1
component: BBa_K1088021

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367840/range2367840/1
type: Range
persistent identity: range2367840
display id: range2367840
version: 1
top level: BBa_K1088007
direction: direction/reverse
start: 201
end: 206
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2367843
display id: annotation2367843
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367843/range2367843/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367843/range2367843/1
type: Range
persistent identity: range2367843
display id: range2367843
version: 1
top level: BBa_K1088007
direction: direction/reverse
start: 207
end: 216
orientation: inline

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation2367845
display id: annotation2367845
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367845/range2367845/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367845/range2367845/1
type: Range
persistent identity: range2367845
display id: range2367845
version: 1
top level: BBa_K1088007
direction: direction/reverse
start: 217
end: 2082
orientation: inline

BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2367846
display id: annotation2367846
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367846/range2367846/1
component: BBa_K105012

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367846/range2367846/1
type: Range
persistent identity: range2367846
display id: range2367846
version: 1
top level: BBa_K1088007
direction: direction/reverse
start: 2083
end: 2112
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2367848
display id: annotation2367848
version: 1
top level: BBa_K1088007
location: https://synbiohub.org/public/igem/BBa_K1088007/annotation2367848/range2367848/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1088007/annotation2367848/range2367848/1
type: Range
persistent identity: range2367848
display id: range2367848
version: 1
top level: BBa_K1088007
direction: direction/reverse
start: 2113
end: 2832
orientation: inline

BBa_K1088019
type: ComponentDefinition
persistent identity: BBa K1088019
display id: BBa_K1088019
version: 1
was generated by: iGEM to SBOL conversion
description: LacI device (constitutive promoter, RBS and terminator)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
mutable description: The trancsriptional repressor lacI (BBa_K1088018)under the constitutivly active promoter (BBa_J23106), with a RBS (BBa_B0030) and with a terminator (BBa_B1002). This device can be used to overexpress lacI with the purpose of repressing transcription from high-copy plasmids containting the lac promoter (BBa_R0010). The repression can be relieved by allosteric binding of IPTG to lacI, thereby promoting transcription from the lac promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Used a strong terminator so that the composite part can be used in front of the lac promoter without unwanted transcription of the regulated gene.
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_B0030
component: BBa_J23106
component: BBa_B1002
component: BBa_K1088018
component: BBa_K1088022
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence annotation: BBa_K1088022
sequence annotation: BBa_K1088018
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1088019_sequence/1

BBa_B0030
type: Component
persistent identity: component2350235
display id: component2350235
version: 1
top level: BBa_K1088019
definition: BBa_B0030
access: public

BBa_J23106
type: Component
persistent identity: component2350233
display id: component2350233
version: 1
top level: BBa_K1088019
definition: BBa_J23106
access: public

BBa_B1002
type: Component
persistent identity: component2350243
display id: component2350243
version: 1
top level: BBa_K1088019
definition: BBa_B1002
access: public

BBa_K1088018
type: Component
persistent identity: component2350238
display id: component2350238
version: 1
top level: BBa_K1088019
definition: lacI
access: public

BBa_K1088022
type: Component
persistent identity: component2350237
display id: component2350237
version: 1
top level: BBa_K1088019
definition: BBa_K1088022
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2350233
display id: annotation2350233
version: 1
top level: BBa_K1088019
location: https://synbiohub.org/public/igem/BBa_K1088019/annotation2350233/range2350233/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1088019/annotation2350233/range2350233/1
type: Range
persistent identity: range2350233
display id: range2350233
version: 1
top level: BBa_K1088019
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2350235
display id: annotation2350235
version: 1
top level: BBa_K1088019
location: https://synbiohub.org/public/igem/BBa_K1088019/annotation2350235/range2350235/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1088019/annotation2350235/range2350235/1
type: Range
persistent identity: range2350235
display id: range2350235
version: 1
top level: BBa_K1088019
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_K1088022
type: SequenceAnnotation
persistent identity: annotation2350237
display id: annotation2350237
version: 1
top level: BBa_K1088019
location: https://synbiohub.org/public/igem/BBa_K1088019/annotation2350237/range2350237/1
component: BBa_K1088022

https://synbiohub.org/public/igem/BBa_K1088019/annotation2350237/range2350237/1
type: Range
persistent identity: range2350237
display id: range2350237
version: 1
top level: BBa_K1088019
direction: direction/reverse
start: 51
end: 56
orientation: inline

BBa_K1088018
type: SequenceAnnotation
persistent identity: annotation2350238
display id: annotation2350238
version: 1
top level: BBa_K1088019
location: https://synbiohub.org/public/igem/BBa_K1088019/annotation2350238/range2350238/1
component: BBa_K1088018

https://synbiohub.org/public/igem/BBa_K1088019/annotation2350238/range2350238/1
type: Range
persistent identity: range2350238
display id: range2350238
version: 1
top level: BBa_K1088019
direction: direction/reverse
start: 57
end: 1139
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2350243
display id: annotation2350243
version: 1
top level: BBa_K1088019
location: https://synbiohub.org/public/igem/BBa_K1088019/annotation2350243/range2350243/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1088019/annotation2350243/range2350243/1
type: Range
persistent identity: range2350243
display id: range2350243
version: 1
top level: BBa_K1088019
direction: direction/reverse
start: 1140
end: 1173
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

dxs
type: ComponentDefinition
persistent identity: BBa K118000
display id: BBa_K118000
version: 1
was generated by: iGEM to SBOL conversion
description: dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of dxs from Escherichia coli JM109. It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses teh first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dxs coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118000_sequence/1

dxs coding sequence
type: SequenceAnnotation
persistent identity: annotation1995699
display id: annotation1995699
version: 1
top level: dxs
location: https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
type: Range
persistent identity: range1995699
display id: range1995699
version: 1
top level: dxs
direction: direction/forward
start: 1
end: 1863
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K105012
type: ComponentDefinition
persistent identity: BBa K105012
display id: BBa_K105012
version: 1
was generated by: iGEM to SBOL conversion
description: 10 aa flexible protein domain linker
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper''
owned by: james
owned by: myers
mutable description: This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n>
discontinued: false
dominant: true
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
star: true
creator: Manuel Gersbacher, Katja Karstens
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_K105012_sequence/1

BBa_K1088021
type: ComponentDefinition
persistent identity: BBa K1088021
display id: BBa_K1088021
version: 1
was generated by: iGEM to SBOL conversion
description: Mixed site (ACTAGA)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Restriction sites
owned by: james
owned by: myers
mutable description: Mixed site (ACTAGA)when XbaI and SpeI sticky ends ligate
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088021_sequence/1

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_K1088022
type: ComponentDefinition
persistent identity: BBa K1088022
display id: BBa_K1088022
version: 1
was generated by: iGEM to SBOL conversion
description: TACTAG
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: TACTAG
owned by: james
owned by: myers
mutable description: Short DNA piece (TACTAG)
discontinued: false
dominant: false
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 6 random nucleotides
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1088022_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa K1088018
display id: BBa_K1088018
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor from E. coli
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: >gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655 http://ecocyc.org/ECOLI/sequence-rc?type=GENE&object=EG10525
owned by: james
owned by: myers
mutable description: Coding region for the LacI protein. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG binds LacI and inhibits its function, and thus promotes transcription from the lac promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: GTG start codon has been changed to ATG
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1088018_sequence/1

https://synbiohub.org/public/igem/BBa_K1088010_sequence/1
type: Sequence
persistent identity: BBa K1088010 sequence
display id: BBa_K1088010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgacgcaaaaaaccccgcttcggcggggttttttcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaactagactcaaggaggatgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088007_sequence/1
type: Sequence
persistent identity: BBa K1088007 sequence
display id: BBa_K1088007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaactagactcaaggaggatgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaagctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcaccgaagaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacggtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgaccaaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagcggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcgaaagacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccggatcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaacccattgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccggtcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacctgcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgccagatgctctataccggctatcactataacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggtcggcgtggaactgacgccgctggaaaaactaccaattggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatccttaactttggtacgctgatgccagaagcggcgaaagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttgtgaaaccgcttgatgaagcgttaattctggaaatggccgccagccatgaagcgctggtcaccgtagaagaaaacgccattatgggcggcgcaggcagcggcgtgaacgaagtgctgatggcccatcgtaaaccagtacccgtgctgaacattggcctgccggacttctttattccgcaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088019_sequence/1
type: Sequence
persistent identity: BBa K1088019 sequence
display id: BBa_K1088019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgacgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118000_sequence/1
type: Sequence
persistent identity: BBa K118000 sequence
display id: BBa_K118000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaagctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcaccgaagaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacggtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgaccaaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagcggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcgaaagacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccggatcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaacccattgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccggtcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacctgcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgccagatgctctataccggctatcactataacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggtcggcgtggaactgacgccgctggaaaaactaccaattggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatccttaactttggtacgctgatgccagaagcggcgaaagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttgtgaaaccgcttgatgaagcgttaattctggaaatggccgccagccatgaagcgctggtcaccgtagaagaaaacgccattatgggcggcgcaggcagcggcgtgaacgaagtgctgatggcccatcgtaaaccagtacccgtgctgaacattggcctgccggacttctttattccgcaaggaactcaggaagaaatgcgcgccgaactcggcctcgatgccgctggtatggaagccaaaatcaaggcctggctggcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105012_sequence/1
type: Sequence
persistent identity: BBa K105012 sequence
display id: BBa_K105012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgaaaatttgtattttcaatctggtggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088021_sequence/1
type: Sequence
persistent identity: BBa K1088021 sequence
display id: BBa_K1088021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088022_sequence/1
type: Sequence
persistent identity: BBa K1088022 sequence
display id: BBa_K1088022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088018_sequence/1
type: Sequence
persistent identity: BBa K1088018 sequence
display id: BBa_K1088018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z