LOCUS       BBa_K1088013            3344 bp    DNA     linear       25-Nov-2024
DEFINITION  B. subtilis dxs (lac promoter with lac inhibitor: IPTG inducible)
ACCESSION   BBa_K1088013
VERSION     BBa_K1088013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    51..56
                     /label=BBa_K1088022
     CDS             57..1184
                     /label=LacI-LVA
     misc_feature    1146..1184
                     /label=LVA
     CDS             57..1184
                     /label=BBa_K1088023
     misc_feature    1185..1218
                     /label=B1002
     stem_loop       1194..1209
                     /label=stem loop
     polyA_site      1209..1215
                     /label=Poly A tail
     polyA_site      1188..1193
                     /label=Poly A tail
     terminator      1185..1218
                     /label=BBa_B1002
     promoter        1..1218
                     /label=BBa_K1088020
     CDS             1227..1314
                     /label=end of LacI coding region (inactive)
     misc_feature    1227..1426
                     /label=BBa_R0010
     misc_feature    1315..1352
                     /label=CAP binding site
     promoter        1363..1368
                     /label=-35
     promoter        1387..1392
                     /label=-10
     misc_feature    1392..1426
                     /label=LacI binding site
     misc_feature    1399..1399
                     /label=start
     promoter        1227..1426
                     /label=BBa_R0010
     misc_feature    1427..1432
                     /label=BBa_K1088021
     misc_feature    1433..1435
                     /label=SacI
     RBS             1436..1442
                     /label=rbs
     RBS             1433..1442
                     /label=BBa_J15001
     CDS             1443..3343
                     /label=Dxs B. sub coding region
     CDS             1443..3344
                     /label=BBa_K1088000
     misc_feature    1227..3344
                     /label=BBa_K1088011
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgg
       61 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
      121 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
      181 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
      241 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
      301 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
      361 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
      421 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
      481 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
      541 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
      601 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
      661 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
      721 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
      781 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
      841 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
      901 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
      961 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     1021 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     1081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1141 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataacgcaaa aaaccccgct
     1201 tcggcggggt tttttcgcta ctagagcaat acgcaaaccg cctctccccg cgcgttggcc
     1261 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1321 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1381 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaacta gactcaagga
     1441 ggatggatct tttatcaata caggacccgt cgtttttaaa aaacatgtcc attgatgaat
     1501 tagagaaatt aagtgatgaa atccgtcagt ttttaattac aagtttatcc gcttccggcg
     1561 gccacatcgg cccaaactta ggtgtcgtag agcttactgt tgccctgcat aaggaattta
     1621 acagcccgaa agacaaattt ttatgggatg taggccatca gtcgtatgtc cataagctgc
     1681 tgacaggacg cggaaaagaa tttgcgacgc ttcgccagta caaagggctt tgcggatttc
     1741 caaagcggag tgaaagcgag cacgatgttt gggaaaccgg gcacagctcg acttctctgt
     1801 caggcgcgat gggaatggca gctgcccgtg atattaaagg aacggatgaa tatattattc
     1861 cgatcattgg tgacggcgcg ctgaccggcg gtatggcgct cgaagccctt aaccacatcg
     1921 gcgacgagaa aaaagacatg attgtcatcc ttaatgataa tgaaatgagt attgcgccaa
     1981 acgtcggtgc cattcactct atgctcggac ggctccgcac tgcggggaaa taccagtggg
     2041 tcaaagatga gcttgaatac ttatttaaaa agattccggc agttgggggc aagcttgccg
     2101 ccacggcgga acgggtcaaa gacagcctga aatacatgct cgtctccgga atgtttttcg
     2161 aggagctcgg ttttacgtat ttgggcccag tggacggaca ttcttatcat gagctgattg
     2221 agaatcttca atacgccaaa aaaacgaaag gccctgttct tctgcacgtc attacgaaaa
     2281 aagggaaggg gtacaaaccg gctgagaccg atacgattgg gacatggcat ggtaccggac
     2341 catataaaat taataccggt gactttgtaa agccgaaagc cgcagctcct tcgtggagcg
     2401 gtcttgtcag cggaactgtg cagcgaatgg cgcgcgagga cggacgcatt gtagccatta
     2461 cgccggctat gcctgtcggt tcaaagcttg aaggcttcgc aaaggaattc cctgaccgga
     2521 tgttcgacgt aggaatcgca gaacagcatg ccgcaacaat ggctgcagct atggcaatgc
     2581 agggtatgaa gccgtttttg gcgatttact caaccttcct gcaaagggca tatgaccaag
     2641 ttgttcatga catctgccgc caaaacgcta atgtgtttat tggaattgac cgtgctggac
     2701 tcgttggcgc tgatggagag acacatcaag gcgtgtttga tattgcgttt atgcgccaca
     2761 ttccaaacat ggtcttaatg atgccgaaag acgaaaatga aggccagcac atggttcata
     2821 cagcacttag ctatgacgaa ggcccgatag caatgcgttt tccgcgcgga aacggactcg
     2881 gcgtaaaaat ggatgaacag ttgaaaacga ttccgatcgg tacgtgggag gtgctgcgtc
     2941 cagggaacga tgctgtcatc ttaacattcg gcacaacaat cgaaatggcg attgaagcag
     3001 ccgaagagct gcagaaagaa ggcctttccg tgcgcgttgt gaatgcgcgt tttattaagc
     3061 cgattgatga aaagatgatg aagagtatcc taaaagaagg cttgccaatt ttaacaattg
     3121 aagaagcggt cttagaaggc ggtttcggaa gctcgatttt agaattcgct catgatcaag
     3181 gtgaatatca tactccgatt gacagaatgg gtatacctga tcggtttatt gaacacggaa
     3241 gtgtaacagc gcttcttgag gaaattggac tgacaaaaca gcaggtggca aatcgtatta
     3301 gattactgat gccaccaaag acacacaaag gaattggatc atga
//