LOCUS BBa_K1088014 3294 bp DNA linear 22-Nov-2024 DEFINITION E. coli dxs (lac promoter with lac inhibitor: IPTG inducible) ACCESSION BBa_K1088014 VERSION BBa_K1088014.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 36..50 /label=RBS-1\Strong RBS 43..47 /label=RBS misc_feature 36..50 /label=BBa_B0030 RBS 36..50 /label=BBa_B0030 misc_feature 51..56 /label=BBa_K1088022 CDS 57..1184 /label=LacI-LVA misc_feature 1146..1184 /label=LVA CDS 57..1184 /label=BBa_K1088023 misc_feature 1185..1218 /label=B1002 stem_loop 1194..1209 /label=stem loop polyA_site 1209..1215 /label=Poly A tail polyA_site 1188..1193 /label=Poly A tail terminator 1185..1218 /label=BBa_B1002 CDS 1219..1306 /label=end of LacI coding region (inactive) misc_feature 1219..1418 /label=BBa_R0010 misc_feature 1307..1344 /label=CAP binding site promoter 1355..1360 /label=-35 promoter 1379..1384 /label=-10 misc_feature 1384..1418 /label=LacI binding site misc_feature 1391..1391 /label=start promoter 1219..1418 /label=BBa_R0010 misc_feature 1419..1421 /label=SacI RBS 1422..1428 /label=rbs RBS 1419..1428 /label=BBa_J15001 CDS 1429..3291 /label=dxs coding sequence CDS 1429..3294 /label=BBa_K118000 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgg 61 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 121 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 181 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 241 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 301 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 361 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 421 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 481 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 541 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 601 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 661 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 721 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 781 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 841 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 901 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 961 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 1021 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 1081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 1141 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataacgcaaa aaaccccgct 1201 tcggcggggt tttttcgcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 1381 tgttgtgtgg aattgtgagc ggataacaat ttcacacact caaggaggat gagttttgat 1441 attgccaaat acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg 1501 aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc 1561 cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac 1621 tatgtctaca acaccccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg 1681 cataaaattt tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggcggtctg 1741 cacccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca 1801 acctccatca gtgccggaat tggtattgcg gttgctgccg aaaaagaagg caaaaatcgc 1861 cgcaccgtct gtgtcattgg cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg 1921 aatcacgcgg gcgatatccg tcctgatatg ctggtgattc tcaacgacaa tgaaatgtcg 1981 atttccgaaa atgtcggcgc gctcaacaac catctggcac agctgctttc cggtaagctt 2041 tactcttcac tgcgcgaagg cgggaaaaaa gttttctctg gcgtgccgcc aattaaagag 2101 ctgctcaaac gcaccgaaga acatattaaa ggcatggtag tgcctggcac gttgtttgaa 2161 gagctgggct ttaactacat cggcccggtg gacggtcacg atgtgctggg gcttatcacc 2221 acgctaaaga acatgcgcga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa 2281 ggtcgtggtt atgaaccggc agaaaaagac ccgatcactt tccacgccgt gcctaaattt 2341 gatccctcca gcggttgttt gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc 2401 tttggcgact ggttgtgcga aacggcagcg aaagacaaca agctgatggc gattactccg 2461 gcgatgcgtg aaggttccgg catggtcgag ttttcacgta aattcccgga tcgctacttc 2521 gacgtggcaa ttgccgagca acacgcggtg acctttgctg cgggtctggc gattggtggg 2581 tacaaaccca ttgtcgcgat ttactccact ttcctgcaac gcgcctatga tcaggtgctg 2641 catgacgtgg cgattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt 2701 ggtgctgacg gtcaaaccca tcagggtgct tttgatctct cttacctgcg ctgcataccg 2761 gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc 2821 tatcactata acgatggccc gtcagcggtg cgctacccgc gtggcaacgc ggtcggcgtg 2881 gaactgacgc cgctggaaaa actaccaatt ggcaaaggca ttgtgaagcg tcgtggcgag 2941 aaactggcga tccttaactt tggtacgctg atgccagaag cggcgaaagt cgccgaatcg 3001 ctgaacgcca cgctggtcga tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg 3061 gaaatggccg ccagccatga agcgctggtc accgtagaag aaaacgccat tatgggcggc 3121 gcaggcagcg gcgtgaacga agtgctgatg gcccatcgta aaccagtacc cgtgctgaac 3181 attggcctgc cggacttctt tattccgcaa ggaactcagg aagaaatgcg cgccgaactc 3241 ggcctcgatg ccgctggtat ggaagccaaa atcaaggcct ggctggcata ataa //