BBa_K1088015
type: ComponentDefinition
persistent identity: BBa K1088015
display id: BBa_K1088015
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG controlled B. sub. dxs coupled with IspG from E. coli
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Despite Dxs from B. sub, it is all from E. coli genome
owned by: james
owned by: myers
mutable description: This parts is the same as BBa_K1088013, but with the IspG gene translationally coupled (seperated by a scarsite) to it.
discontinued: false
dominant: false
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Look at the design considerations for BBa_K1088013 for more info. The IspG is conventionally cloned so there is a scarsite between the Dxs and IspG gene.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K1088004
component: BBa_K1088013
sequence annotation: BBa_K1088013
sequence annotation: BBa_K1088004
sequence: https://synbiohub.org/public/igem/BBa_K1088015_sequence/1

BBa_K1088004
type: Component
persistent identity: component2374417
display id: component2374417
version: 1
top level: BBa_K1088015
definition: BBa_K1088004
access: public

BBa_K1088013
type: Component
persistent identity: component2374415
display id: component2374415
version: 1
top level: BBa_K1088015
definition: BBa_K1088013
access: public

BBa_K1088013
type: SequenceAnnotation
persistent identity: annotation2374415
display id: annotation2374415
version: 1
top level: BBa_K1088015
location: https://synbiohub.org/public/igem/BBa_K1088015/annotation2374415/range2374415/1
component: BBa_K1088013

https://synbiohub.org/public/igem/BBa_K1088015/annotation2374415/range2374415/1
type: Range
persistent identity: range2374415
display id: range2374415
version: 1
top level: BBa_K1088015
direction: direction/reverse
start: 1
end: 3344
orientation: inline

BBa_K1088004
type: SequenceAnnotation
persistent identity: annotation2374417
display id: annotation2374417
version: 1
top level: BBa_K1088015
location: https://synbiohub.org/public/igem/BBa_K1088015/annotation2374417/range2374417/1
component: BBa_K1088004

https://synbiohub.org/public/igem/BBa_K1088015/annotation2374417/range2374417/1
type: Range
persistent identity: range2374417
display id: range2374417
version: 1
top level: BBa_K1088015
direction: direction/reverse
start: 3351
end: 4469
orientation: inline

BBa_K1088004
type: ComponentDefinition
persistent identity: BBa K1088004
display id: BBa_K1088004
version: 1
was generated by: iGEM to SBOL conversion
description: IspG (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase)
created: 2013-08-05T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Isolated from E. coli MG1655
owned by: james
owned by: myers
mutable description: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) is a part of the bacterial MEP pathway used for production of isoprenoids. From ecocyc: "IspG catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. IspG activity depends on as-yet unidentified additional proteins, most likely involved in the oxidation portion of the reaction" http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10370
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We designed primers to isolate IspG directly from the genome by colony PCR. The primers included an overhang containing the biobrick prefix and suffix
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ispG
sequence: https://synbiohub.org/public/igem/BBa_K1088004_sequence/1

ispG
type: SequenceAnnotation
persistent identity: annotation2331287
display id: annotation2331287
version: 1
top level: BBa_K1088004
location: https://synbiohub.org/public/igem/BBa_K1088004/annotation2331287/range2331287/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1088004/annotation2331287/range2331287/1
type: Range
persistent identity: range2331287
display id: range2331287
version: 1
top level: BBa_K1088004
direction: direction/forward
start: 1
end: 1119
orientation: inline

BBa_K1088013
type: ComponentDefinition
persistent identity: BBa K1088013
display id: BBa_K1088013
version: 1
was generated by: iGEM to SBOL conversion
description: B. subtilis dxs (lac promoter with lac inhibitor: IPTG inducible)
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: All but the B. sub Dxs originates from the E. coli genome
owned by: james
owned by: myers
mutable description: This system allows IPTG controlled expression of the Dxs enzyme isolated from B. sub.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There is a scarsite between the RBS (BBa_B0030) and LacI aswell as a scarsite before and after the RBS of B. sub. Dxs
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1088011
component: BBa_K1088020
sequence annotation: BBa_K1088020
sequence annotation: BBa_K1088011
sequence: https://synbiohub.org/public/igem/BBa_K1088013_sequence/1

BBa_K1088011
type: Component
persistent identity: component2374386
display id: component2374386
version: 1
top level: BBa_K1088013
definition: BBa_K1088011
access: public

BBa_K1088020
type: Component
persistent identity: component2374372
display id: component2374372
version: 1
top level: BBa_K1088013
definition: BBa_K1088020
access: public

BBa_K1088020
type: SequenceAnnotation
persistent identity: annotation2374372
display id: annotation2374372
version: 1
top level: BBa_K1088013
location: https://synbiohub.org/public/igem/BBa_K1088013/annotation2374372/range2374372/1
component: BBa_K1088020

https://synbiohub.org/public/igem/BBa_K1088013/annotation2374372/range2374372/1
type: Range
persistent identity: range2374372
display id: range2374372
version: 1
top level: BBa_K1088013
direction: direction/reverse
start: 1
end: 1218
orientation: inline

BBa_K1088011
type: SequenceAnnotation
persistent identity: annotation2374386
display id: annotation2374386
version: 1
top level: BBa_K1088013
location: https://synbiohub.org/public/igem/BBa_K1088013/annotation2374386/range2374386/1
component: BBa_K1088011

https://synbiohub.org/public/igem/BBa_K1088013/annotation2374386/range2374386/1
type: Range
persistent identity: range2374386
display id: range2374386
version: 1
top level: BBa_K1088013
direction: direction/reverse
start: 1227
end: 3344
orientation: inline

BBa_K1088020
type: ComponentDefinition
persistent identity: BBa K1088020
display id: BBa_K1088020
version: 1
was generated by: iGEM to SBOL conversion
description: lacI:LVA device (constitutive promoter, RBS and terminator)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
mutable description: The trancsriptional repressor lacI with LVA tag for faster degradation (BBa_C0012)under the constitutivly active promoter (BBa_J23106), with a RBS (BBa_B0030) and with a terminator (BBa_B1002). This device can be used to overexpress lacI with the purpose of repressing transcription from high-copy plasmids containting the lac promoter (BBa_R0010). The repression can be relieved by allosteric binding of IPTG to lacI, thereby promoting transcription from the lac promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Used a strong terminator so that the composite part can be used in front of the lac promoter without unwanted transcription of the regulated gene.
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_J23106
component: BBa_B1002
component: BBa_K1088023
component: BBa_K1088022
component: BBa_B0030
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence annotation: BBa_K1088022
sequence annotation: BBa_K1088023
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1088020_sequence/1

BBa_J23106
type: Component
persistent identity: component2351520
display id: component2351520
version: 1
top level: BBa_K1088020
definition: BBa_J23106
access: public

BBa_B1002
type: Component
persistent identity: component2351532
display id: component2351532
version: 1
top level: BBa_K1088020
definition: BBa_B1002
access: public

BBa_K1088023
type: Component
persistent identity: component2351527
display id: component2351527
version: 1
top level: BBa_K1088020
definition: lacI-LVA
access: public

BBa_K1088022
type: Component
persistent identity: component2351524
display id: component2351524
version: 1
top level: BBa_K1088020
definition: BBa_K1088022
access: public

BBa_B0030
type: Component
persistent identity: component2351522
display id: component2351522
version: 1
top level: BBa_K1088020
definition: BBa_B0030
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2351520
display id: annotation2351520
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351520/range2351520/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351520/range2351520/1
type: Range
persistent identity: range2351520
display id: range2351520
version: 1
top level: BBa_K1088020
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2351522
display id: annotation2351522
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351522/range2351522/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351522/range2351522/1
type: Range
persistent identity: range2351522
display id: range2351522
version: 1
top level: BBa_K1088020
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_K1088022
type: SequenceAnnotation
persistent identity: annotation2351524
display id: annotation2351524
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351524/range2351524/1
component: BBa_K1088022

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351524/range2351524/1
type: Range
persistent identity: range2351524
display id: range2351524
version: 1
top level: BBa_K1088020
direction: direction/reverse
start: 51
end: 56
orientation: inline

BBa_K1088023
type: SequenceAnnotation
persistent identity: annotation2351527
display id: annotation2351527
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351527/range2351527/1
component: BBa_K1088023

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351527/range2351527/1
type: Range
persistent identity: range2351527
display id: range2351527
version: 1
top level: BBa_K1088020
direction: direction/reverse
start: 57
end: 1184
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2351532
display id: annotation2351532
version: 1
top level: BBa_K1088020
location: https://synbiohub.org/public/igem/BBa_K1088020/annotation2351532/range2351532/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1088020/annotation2351532/range2351532/1
type: Range
persistent identity: range2351532
display id: range2351532
version: 1
top level: BBa_K1088020
direction: direction/reverse
start: 1185
end: 1218
orientation: inline

BBa_K1088011
type: ComponentDefinition
persistent identity: BBa K1088011
display id: BBa_K1088011
version: 1
was generated by: iGEM to SBOL conversion
description: B. subtilis dxs (lac promoter without lac inhibitor)
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: The Lac promoter derives from E. coli. The Dxs protein is from B. sub
owned by: james
owned by: myers
mutable description: This part has the B. sub dxs (BBa_K1088000) controlled by the common Lac promoter. Notice that there is no LacI in this system, which is required for regulation of the Lac promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We forgot to acknowledge the need for high expression of LacI to keep the regulation of the lac promoter vaild. The constructs where assembled with USER cloning, and therefore there are no scarsites between the parts. There are however inserted scarsites between the RBS and the coding region aswell as between the lac promoter and the RBS to improve translational efficiency
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1088021
component: BBa_R0010
component: BBa_K1088000
component: BBa_J15001
sequence annotation: BBa_R0010
sequence annotation: BBa_K1088021
sequence annotation: BBa_J15001
sequence annotation: BBa_K1088000
sequence: https://synbiohub.org/public/igem/BBa_K1088011_sequence/1

BBa_K1088021
type: Component
persistent identity: component2369133
display id: component2369133
version: 1
top level: BBa_K1088011
definition: BBa_K1088021
access: public

BBa_R0010
type: Component
persistent identity: component2369126
display id: component2369126
version: 1
top level: BBa_K1088011
definition: LacI
access: public

BBa_K1088000
type: Component
persistent identity: component2369138
display id: component2369138
version: 1
top level: BBa_K1088011
definition: dxs (B. su
access: public

BBa_J15001
type: Component
persistent identity: component2369136
display id: component2369136
version: 1
top level: BBa_K1088011
definition: BBa_J15001
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2369126
display id: annotation2369126
version: 1
top level: BBa_K1088011
location: https://synbiohub.org/public/igem/BBa_K1088011/annotation2369126/range2369126/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1088011/annotation2369126/range2369126/1
type: Range
persistent identity: range2369126
display id: range2369126
version: 1
top level: BBa_K1088011
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1088021
type: SequenceAnnotation
persistent identity: annotation2369133
display id: annotation2369133
version: 1
top level: BBa_K1088011
location: https://synbiohub.org/public/igem/BBa_K1088011/annotation2369133/range2369133/1
component: BBa_K1088021

https://synbiohub.org/public/igem/BBa_K1088011/annotation2369133/range2369133/1
type: Range
persistent identity: range2369133
display id: range2369133
version: 1
top level: BBa_K1088011
direction: direction/reverse
start: 201
end: 206
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2369136
display id: annotation2369136
version: 1
top level: BBa_K1088011
location: https://synbiohub.org/public/igem/BBa_K1088011/annotation2369136/range2369136/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K1088011/annotation2369136/range2369136/1
type: Range
persistent identity: range2369136
display id: range2369136
version: 1
top level: BBa_K1088011
direction: direction/reverse
start: 207
end: 216
orientation: inline

BBa_K1088000
type: SequenceAnnotation
persistent identity: annotation2369138
display id: annotation2369138
version: 1
top level: BBa_K1088011
location: https://synbiohub.org/public/igem/BBa_K1088011/annotation2369138/range2369138/1
component: BBa_K1088000

https://synbiohub.org/public/igem/BBa_K1088011/annotation2369138/range2369138/1
type: Range
persistent identity: range2369138
display id: range2369138
version: 1
top level: BBa_K1088011
direction: direction/reverse
start: 217
end: 2118
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

dxs (B. su
type: ComponentDefinition
persistent identity: BBa K1088000
display id: BBa_K1088000
version: 1
was generated by: iGEM to SBOL conversion
description: 1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168
created: 2013-07-08T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: B. subtilis 168
owned by: james
owned by: myers
mutable description: The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs)gene aswell as the second step of the MEP pathway. They saw a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway). From Biocyc: "Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways." -http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: we changed TTG start codon to ATG to improve translation efficiency. We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Dxs B. sub coding region
sequence: https://synbiohub.org/public/igem/BBa_K1088000_sequence/1

Dxs B. sub coding region
type: SequenceAnnotation
persistent identity: annotation2331286
display id: annotation2331286
version: 1
top level: dxs (B. su
location: https://synbiohub.org/public/igem/BBa_K1088000/annotation2331286/range2331286/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1088000/annotation2331286/range2331286/1
type: Range
persistent identity: range2331286
display id: range2331286
version: 1
top level: dxs (B. su
direction: direction/forward
start: 1
end: 1901
orientation: inline

BBa_K1088021
type: ComponentDefinition
persistent identity: BBa K1088021
display id: BBa_K1088021
version: 1
was generated by: iGEM to SBOL conversion
description: Mixed site (ACTAGA)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Restriction sites
owned by: james
owned by: myers
mutable description: Mixed site (ACTAGA)when XbaI and SpeI sticky ends ligate
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088021_sequence/1

BBa_K1088022
type: ComponentDefinition
persistent identity: BBa K1088022
display id: BBa_K1088022
version: 1
was generated by: iGEM to SBOL conversion
description: TACTAG
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: TACTAG
owned by: james
owned by: myers
mutable description: Short DNA piece (TACTAG)
discontinued: false
dominant: false
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 6 random nucleotides
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088022_sequence/1

lacI-LVA
type: ComponentDefinition
persistent identity: BBa K1088023
display id: BBa_K1088023
version: 1
was generated by: iGEM to SBOL conversion
description: LacI-LVA (part C0012 without barcodes)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
mutable description: See BBa_C0012 Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LacI-LVA
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K1088023_sequence/1

LacI-LVA
type: SequenceAnnotation
persistent identity: annotation2351490
display id: annotation2351490
version: 1
top level: lacI-LVA
location: https://synbiohub.org/public/igem/BBa_K1088023/annotation2351490/range2351490/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1088023/annotation2351490/range2351490/1
type: Range
persistent identity: range2351490
display id: range2351490
version: 1
top level: lacI-LVA
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2351491
display id: annotation2351491
version: 1
top level: lacI-LVA
location: https://synbiohub.org/public/igem/BBa_K1088023/annotation2351491/range2351491/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1088023/annotation2351491/range2351491/1
type: Range
persistent identity: range2351491
display id: range2351491
version: 1
top level: lacI-LVA
direction: direction/forward
start: 1090
end: 1128
orientation: inline

https://synbiohub.org/public/igem/BBa_K1088015_sequence/1
type: Sequence
persistent identity: BBa K1088015 sequence
display id: BBa_K1088015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataacgcaaaaaaccccgcttcggcggggttttttcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaactagactcaaggaggatggatcttttatcaatacaggacccgtcgtttttaaaaaacatgtccattgatgaattagagaaattaagtgatgaaatccgtcagtttttaattacaagtttatccgcttccggcggccacatcggcccaaacttaggtgtcgtagagcttactgttgccctgcataaggaatttaacagcccgaaagacaaatttttatgggatgtaggccatcagtcgtatgtccataagctgctgacaggacgcggaaaagaatttgcgacgcttcgccagtacaaagggctttgcggatttccaaagcggagtgaaagcgagcacgatgtttgggaaaccgggcacagctcgacttctctgtcaggcgcgatgggaatggcagctgcccgtgatattaaaggaacggatgaatatattattccgatcattggtgacggcgcgctgaccggcggtatggcgctcgaagcccttaaccacatcggcgacgagaaaaaagacatgattgtcatccttaatgataatgaaatgagtattgcgccaaacgtcggtgccattcactct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088004_sequence/1
type: Sequence
persistent identity: BBa K1088004 sequence
display id: BBa_K1088004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcataaccaggctccaattcaacgtagaaaatcaacacgtatttacgttgggaatgtgccgattggcgatggtgctcccatcgccgtacagtccatgaccaatacgcgtacgacagacgtcgaagcaacggtcaatcaaatcaaggcgctggaacgcgttggcgctgatatcgtccgtgtatccgtaccgacgatggacgcggcagaagcgttcaaactcatcaaacagcaggttaacgtgccgctggtggctgacatccacttcgactatcgcattgcgctgaaagtagcggaatacggcgtcgattgtctgcgtattaaccctggcaatatcggtaatgaagagcgtattcgcatggtggttgactgtgcgcgcgataaaaacattccgatccgtattggcgttaacgccggatcgctggaaaaagatctgcaagaaaagtatggcgaaccgacgccgcaggcgttgctggaatctgccatgcgtcatgttgatcatctcgatcgcctgaacttcgatcagttcaaagtcagcgtgaaagcgtctgacgtcttcctcgctgttgagtcttatcgtttgctggcaaaacagatcgatcagccgttgcatctggggatcaccgaagccggtggtgcgcgcagcggggcagtaaaatccgccattggtttaggtctgctgctgtctgaaggcatcggcgacacgctgcgcgtatcgctggcggccgatccggtcgaagagatcaaagtcggtttcgatattttgaaatcgctgcgtatccgttcgcgagggatcaacttcatcgcctgcccgacctgttcgcgtcaggaatttgatgttatcggtacggttaacgcgctggagcaacgcctggaagatatcatcactccgatggacgtttcgattatcggctgcgtggtgaatggcccaggtgaggcgctggtttctacactcggcgtcaccggcggcaacaagaaaagcggcctctatgaagatggcgtgcgcaaagaccgtctggacaacaacgatatgatcgaccagctggaagcacgcattcgtgcgaaagccagtcagctggacgaagcgcgtcgaattgacgttcagcaggttgaaaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088013_sequence/1
type: Sequence
persistent identity: BBa K1088013 sequence
display id: BBa_K1088013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataacgcaaaaaaccccgcttcggcggggttttttcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaactagactcaaggaggatggatcttttatcaatacaggacccgtcgtttttaaaaaacatgtccattgatgaattagagaaattaagtgatgaaatccgtcagtttttaattacaagtttatccgcttccggcggccacatcggcccaaacttaggtgtcgtagagcttactgttgccctgcataaggaatttaacagcccgaaagacaaatttttatgggatgtaggccatcagtcgtatgtccataagctgctgacaggacgcggaaaagaatttgcgacgcttcgccagtacaaagggctttgcggatttccaaagcggagtgaaagcgagcacgatgtttgggaaaccgggcacagctcgacttctctgtcaggcgcgatgggaatggcagctgcccgtgatattaaaggaacggatgaatatattattccgatcattggtgacggcgcgctgaccggcggtatggcgctcgaagcccttaaccacatcggcgacgagaaaaaagacatgattgtcatccttaatgataatgaaatgagtattgcgccaaacgtcggtgccattcactct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088020_sequence/1
type: Sequence
persistent identity: BBa K1088020 sequence
display id: BBa_K1088020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataacgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088011_sequence/1
type: Sequence
persistent identity: BBa K1088011 sequence
display id: BBa_K1088011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaactagactcaaggaggatggatcttttatcaatacaggacccgtcgtttttaaaaaacatgtccattgatgaattagagaaattaagtgatgaaatccgtcagtttttaattacaagtttatccgcttccggcggccacatcggcccaaacttaggtgtcgtagagcttactgttgccctgcataaggaatttaacagcccgaaagacaaatttttatgggatgtaggccatcagtcgtatgtccataagctgctgacaggacgcggaaaagaatttgcgacgcttcgccagtacaaagggctttgcggatttccaaagcggagtgaaagcgagcacgatgtttgggaaaccgggcacagctcgacttctctgtcaggcgcgatgggaatggcagctgcccgtgatattaaaggaacggatgaatatattattccgatcattggtgacggcgcgctgaccggcggtatggcgctcgaagcccttaaccacatcggcgacgagaaaaaagacatgattgtcatccttaatgataatgaaatgagtattgcgccaaacgtcggtgccattcactctatgctcggacggctccgcactgcggggaaataccagtgggtcaaagatgagcttgaatacttatttaaaaagattccggcagttgggggcaagcttgccgccacggcggaacgggtcaaagacagcctgaaatacatgctcgtctccggaatgtttttcgaggagctcggttttacgtatttgggcccagtggacggacattcttatcatgagctgattgagaatcttcaatacgccaaaaaaacgaaaggccctgttcttctgcacgtcattacgaaaaaagggaaggggtacaaaccggctgagaccgatacgattgggacatggcatggtaccggaccatataaaattaataccggtgactttgtaaagccgaaagccgcagctccttcgtggagcggtcttgtcagcggaactgtgcagcgaatggcgcgcgaggacggacgcattgtagccattacgccggctatgcctgtcggttcaaagcttgaaggcttcgcaaaggaattccctgaccggatgttcgacgtaggaatcgcagaacagcatgccgcaacaatggctgcagctatggcaatgcagggtatgaagccgtttttggcgatttactcaaccttcctgcaaagggcatatgaccaagttgttcatgacatctgccgccaaaacgctaatgtgtttattggaattgaccgtgctggactcgttggcgctgatggagagacacatcaaggcgtgtttgatattgcgtttatgcgccacattccaaacatggtcttaatgatgccgaaagacgaaaatgaaggccagcacatggttcatacagcacttagctatgacgaaggcccgatagcaatgcgttttccgcgcggaaacggactcggcgtaaaaatggatgaacagttgaaaacgattccgatcggtacgtgggaggtgctgcgtccagggaacgatgctgtcatcttaacattcggcacaacaatcgaaatggcgattgaagcagccgaagagctgcagaaagaaggcctttccgtgcgcgttgtgaatgcgcgttttattaagccgattgatgaaaagatgatgaagagtatcctaaaagaaggcttgccaattttaacaattgaagaagcggtcttagaaggcggtttcggaagctcgattttagaattcgctcatgatcaaggtgaatatcatactccgattgacagaatgggtatacctgatcggtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088000_sequence/1
type: Sequence
persistent identity: BBa K1088000 sequence
display id: BBa_K1088000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggatcttttatcaatacaggacccgtcgtttttaaaaaacatgtccattgatgaattagagaaattaagtgatgaaatccgtcagtttttaattacaagtttatccgcttccggcggccacatcggcccaaacttaggtgtcgtagagcttactgttgccctgcataaggaatttaacagcccgaaagacaaatttttatgggatgtaggccatcagtcgtatgtccataagctgctgacaggacgcggaaaagaatttgcgacgcttcgccagtacaaagggctttgcggatttccaaagcggagtgaaagcgagcacgatgtttgggaaaccgggcacagctcgacttctctgtcaggcgcgatgggaatggcagctgcccgtgatattaaaggaacggatgaatatattattccgatcattggtgacggcgcgctgaccggcggtatggcgctcgaagcccttaaccacatcggcgacgagaaaaaagacatgattgtcatccttaatgataatgaaatgagtattgcgccaaacgtcggtgccattcactctatgctcggacggctccgcactgcggggaaataccagtgggtcaaagatgagcttgaatacttatttaaaaagattccggcagttgggggcaagcttgccgccacggcggaacgggtcaaagacagcctgaaatacatgctcgtctccggaatgtttttcgaggagctcggttttacgtatttgggcccagtggacggacattcttatcatgagctgattgagaatcttcaatacgccaaaaaaacgaaaggccctgttcttctgcacgtcattacgaaaaaagggaaggggtacaaaccggctgagaccgatacgattgggacatggcatggtaccggaccatataaaattaataccggtgactttgtaaagccgaaagccgcagctccttcgtggagcggtcttgtcagcggaactgtgcagcgaatggcgcgcgaggacggacgcattgtagccattacgccggctatgcctgtcggttcaaagcttgaaggcttcgcaaaggaattccctgaccggatgttcgacgtaggaatcgcagaacagcatgccgcaacaatggctgcagctatggcaatgcagggtatgaagccgtttttggcgatttactcaaccttcctgcaaagggcatatgaccaagttgttcatgacatctgccgccaaaacgctaatgtgtttattggaattgaccgtgctggactcgttggcgctgatggagagacacatcaaggcgtgtttgatattgcgtttatgcgccacattccaaacatggtcttaatgatgccgaaagacgaaaatgaaggccagcacatggttcatacagcacttagctatgacgaaggcccgatagcaatgcgttttccgcgcggaaacggactcggcgtaaaaatggatgaacagttgaaaacgattccgatcggtacgtgggaggtgctgcgtccagggaacgatgctgtcatcttaacattcggcacaacaatcgaaatggcgattgaagcagccgaagagctgcagaaagaaggcctttccgtgcgcgttgtgaatgcgcgttttattaagccgattgatgaaaagatgatgaagagtatcctaaaagaaggcttgccaattttaacaattgaagaagcggtcttagaaggcggtttcggaagctcgattttagaattcgctcatgatcaaggtgaatatcatactccgattgacagaatgggtatacctgatcggtttattgaacacggaagtgtaacagcgcttcttgaggaaattggactgacaaaacagcaggtggcaaatcgtattagattactgatgccaccaaagacacacaaaggaattggatcatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088021_sequence/1
type: Sequence
persistent identity: BBa K1088021 sequence
display id: BBa_K1088021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088022_sequence/1
type: Sequence
persistent identity: BBa K1088022 sequence
display id: BBa_K1088022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088023_sequence/1
type: Sequence
persistent identity: BBa K1088023 sequence
display id: BBa_K1088023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z