BBa_K1088017 1 AraC AraC device (constitutive promoter, RBS and terminator) 2013-09-15T11:00:00Z 2015-05-08T01:09:06Z >gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655 Coding region for the LacI protein derived from E. coli. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG (Isopropyl &#946;-D-1-thiogalactopyranoside) binds to LacI and inhibits its operation, thereby promoting transcription. false false _1398_ 0 17057 9 In stock false The GTG start codon was changed into ATG false Patrick Rosendahl Andreassen component2352408 1 BBa_K1088005 component2352406 1 BBa_K1088022 component2352402 1 BBa_J23106 component2352404 1 BBa_B0030 component2352413 1 BBa_B1002 annotation2352406 1 BBa_K1088022 range2352406 1 51 56 annotation2352413 1 BBa_B1002 range2352413 1 936 969 annotation2352404 1 BBa_B0030 range2352404 1 36 50 annotation2352402 1 BBa_J23106 range2352402 1 1 35 annotation2352408 1 BBa_K1088005 range2352408 1 57 935 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1088003 1 HRT2 Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA 2013-07-09T11:00:00Z 2015-05-08T01:09:06Z The gene is synthesized by http://www.genscript.com/ This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons. false false _1398_ 0 12807 9 In stock false We optimized codons to fit the most used codons of E. coli. false Andreas Kj??r, Patrick Rosendahl Andreassen BBa_K1088016 1 BBa_K1088016 HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible) 2013-08-27T11:00:00Z 2015-05-08T01:09:06Z Apart from the prenyltransferase all the parts derive from E. coli. The prenyltransferase have been synthesized based on the cDNA sequence of a Hevea brasiliensis latex extract study. For more info, look at BBa_K1088003. This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with IPTG. false false _1398_ 0 12807 9 In stock true There is a scarsite between the RBS of araC (BBa_B0030) aswell as in front of and behind the RBS of the prenyltransferase to improve translational efficieny. There are no more scarsites because the rest is assembled with USER cloning false Andreas Kj??r component2376941 1 BBa_K1088024 component2376933 1 BBa_K1088017 annotation2376941 1 BBa_K1088024 range2376941 1 978 2044 annotation2376933 1 BBa_K1088017 range2376933 1 1 969 BBa_B1002 1 BBa_B1002 Terminator (artificial, small, %T~=85%) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artifical terminator, estimated %T~=85 false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues. true Haiyao Huang annotation1898412 1 B1002 range1898412 1 1 34 annotation1898414 1 Poly A tail range1898414 1 25 31 annotation1898415 1 Poly A tail range1898415 1 4 9 annotation1898413 1 stem loop range1898413 1 10 25 BBa_K1088005 1 araC arabinose regulator (araC) coding sequence 2013-08-19T11:00:00Z 2015-05-08T01:09:06Z E. coli K-12 MG1655 This sequence is a copy of the sequence 70,387 -> 71,265 in E. coli K-12 MG1655 which is the coding sequence of the pBAD repressor araC. From ECOCYC: The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. It coregulates with another transcriptional regulator, CRP; both are transcription factors involved in l-arabinose degradation. These regulators bind cooperatively to activate transcription of five operons related to transport, catabolism, and autoregulation of l-arabinose. Transcription of these operons is induced when E. coli is grown in the absence of glucose and when the physiological inducer, l-arabinose, binds to the AraC regulator. In the absence of glucose, cellular cyclic AMP levels are high and cyclic AMP forms a dimeric complex with CRP to coregulate with AraC. For more info go to http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10054 false false _1398_ 0 12807 9 Not in stock false The part was amplified from chromosomal DNA with primers carrying prefix and suffix overhangs false Andreas Kj??r annotation2331909 1 araC coding region range2331909 1 1 879 BBa_K1088024 1 Rubber Fuc HRT2 prenyltransferase from Hevea Brasilianis (ara promoter without araC: arabinose inducible) 2013-09-17T11:00:00Z 2015-05-08T01:09:06Z Source is specified in components This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The gene is expressed from the arabinose promoter (BBa_I13453), which is induceable with arabinose under glucose scarce conditions. Note that the regulater insn't a part of this part and thus no/very little transcription is expected. false false _1398_ 0 12807 9 It's complicated false There is a 3xFLAG-tag after the HRT2 stop codon, and thus the 3xFLAG-tag isn't translated. false Andreas Kj??r component2373590 1 BBa_J15001 component2373586 1 BBa_I13453 component2373587 1 BBa_K1088021 component2373592 1 BBa_K1088025 component2373591 1 BBa_K1088003 annotation2373591 1 BBa_K1088003 range2373591 1 147 1001 annotation2373592 1 BBa_K1088025 range2373592 1 1002 1067 annotation2373587 1 BBa_K1088021 range2373587 1 131 136 annotation2373590 1 BBa_J15001 range2373590 1 137 146 annotation2373586 1 BBa_I13453 range2373586 1 1 130 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_K1088021 1 BBa_K1088021 Mixed site (ACTAGA) 2013-09-16T11:00:00Z 2015-05-08T01:09:06Z Restriction sites Mixed site (ACTAGA)when XbaI and SpeI sticky ends ligate false false _1398_ 0 17057 9 Not in stock false None false Patrick Rosendahl Andreassen BBa_K1088022 1 BBa_K1088022 TACTAG 2013-09-16T11:00:00Z 2015-05-08T01:09:06Z TACTAG Short DNA piece (TACTAG) false false _1398_ 0 17057 9 Not in stock false 6 random nucleotides false Patrick Rosendahl Andreassen BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1088025 1 3xFLAG 3xFLAG 2013-09-17T11:00:00Z 2015-05-08T01:09:06Z Sigma Aldrich 3xFLAG tag that can be fused with proteins either at the C- or N-terminal end. It can be used to purify the tagged protein or check levels of expression of tagged protein. false false _1398_ 0 12807 9 Not in stock false None false Andreas Kj??r BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938045 1 SacI range1938045 1 1 3 annotation1938046 1 rbs range1938046 1 4 10 BBa_K1088016_sequence 1 tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataacgcaaaaaaccccgcttcggcggggttttttcgctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcactagactcaaggaggatggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataagactacaaagaccatgacggtgattataaagatcatgatatcgactacaaagatgacgacgataaa BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K1088024_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcactagactcaaggaggatggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataagactacaaagaccatgacggtgattataaagatcatgatatcgactacaaagatgacgacgataaa BBa_K1088003_sequence 1 atggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataa BBa_J15001_sequence 1 ctcaaggagg BBa_K1088021_sequence 1 actaga BBa_K1088025_sequence 1 gactacaaagaccatgacggtgattataaagatcatgatatcgactacaaagatgacgacgataaa BBa_B1002_sequence 1 cgcaaaaaaccccgcttcggcggggttttttcgc BBa_K1088022_sequence 1 tactag BBa_K1088017_sequence 1 tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataacgcaaaaaaccccgcttcggcggggttttttcgc BBa_B0030_sequence 1 attaaagaggagaaa BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K1088005_sequence 1 atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z