BBa_K1088016
type: ComponentDefinition
persistent identity: BBa K1088016
display id: BBa_K1088016
version: 1
was generated by: iGEM to SBOL conversion
description: HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Apart from the prenyltransferase all the parts derive from E. coli. The prenyltransferase have been synthesized based on the cDNA sequence of a Hevea brasiliensis latex extract study. For more info, look at BBa_K1088003.
owned by: james
owned by: myers
mutable description: This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with IPTG.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a scarsite between the RBS of araC (BBa_B0030) aswell as in front of and behind the RBS of the prenyltransferase to improve translational efficieny. There are no more scarsites because the rest is assembled with USER cloning
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1088024
component: BBa_K1088017
sequence annotation: BBa_K1088017
sequence annotation: BBa_K1088024
sequence: https://synbiohub.org/public/igem/BBa_K1088016_sequence/1

BBa_K1088024
type: Component
persistent identity: component2376941
display id: component2376941
version: 1
top level: BBa_K1088016
definition: Rubber Fuc
access: public

BBa_K1088017
type: Component
persistent identity: component2376933
display id: component2376933
version: 1
top level: BBa_K1088016
definition: AraC
access: public

BBa_K1088017
type: SequenceAnnotation
persistent identity: annotation2376933
display id: annotation2376933
version: 1
top level: BBa_K1088016
location: https://synbiohub.org/public/igem/BBa_K1088016/annotation2376933/range2376933/1
component: BBa_K1088017

https://synbiohub.org/public/igem/BBa_K1088016/annotation2376933/range2376933/1
type: Range
persistent identity: range2376933
display id: range2376933
version: 1
top level: BBa_K1088016
direction: direction/reverse
start: 1
end: 969
orientation: inline

BBa_K1088024
type: SequenceAnnotation
persistent identity: annotation2376941
display id: annotation2376941
version: 1
top level: BBa_K1088016
location: https://synbiohub.org/public/igem/BBa_K1088016/annotation2376941/range2376941/1
component: BBa_K1088024

https://synbiohub.org/public/igem/BBa_K1088016/annotation2376941/range2376941/1
type: Range
persistent identity: range2376941
display id: range2376941
version: 1
top level: BBa_K1088016
direction: direction/reverse
start: 978
end: 2044
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa K1088017
display id: BBa_K1088017
version: 1
was generated by: iGEM to SBOL conversion
description: AraC device (constitutive promoter, RBS and terminator)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: >gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: Coding region for the LacI protein derived from E. coli. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG (Isopropyl β-D-1-thiogalactopyranoside) binds to LacI and inhibits its operation, thereby promoting transcription.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The GTG start codon was changed into ATG
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K1088022
component: BBa_B0030
component: BBa_J23106
component: BBa_B1002
component: BBa_K1088005
sequence annotation: BBa_J23106
sequence annotation: BBa_B0030
sequence annotation: BBa_K1088022
sequence annotation: BBa_K1088005
sequence annotation: BBa_B1002
sequence: https://synbiohub.org/public/igem/BBa_K1088017_sequence/1

BBa_K1088022
type: Component
persistent identity: component2352406
display id: component2352406
version: 1
top level: AraC
definition: BBa_K1088022
access: public

BBa_B0030
type: Component
persistent identity: component2352404
display id: component2352404
version: 1
top level: AraC
definition: BBa_B0030
access: public

BBa_J23106
type: Component
persistent identity: component2352402
display id: component2352402
version: 1
top level: AraC
definition: BBa_J23106
access: public

BBa_B1002
type: Component
persistent identity: component2352413
display id: component2352413
version: 1
top level: AraC
definition: BBa_B1002
access: public

BBa_K1088005
type: Component
persistent identity: component2352408
display id: component2352408
version: 1
top level: AraC
definition: araC
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2352402
display id: annotation2352402
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_K1088017/annotation2352402/range2352402/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1088017/annotation2352402/range2352402/1
type: Range
persistent identity: range2352402
display id: range2352402
version: 1
top level: AraC
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2352404
display id: annotation2352404
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_K1088017/annotation2352404/range2352404/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1088017/annotation2352404/range2352404/1
type: Range
persistent identity: range2352404
display id: range2352404
version: 1
top level: AraC
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_K1088022
type: SequenceAnnotation
persistent identity: annotation2352406
display id: annotation2352406
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_K1088017/annotation2352406/range2352406/1
component: BBa_K1088022

https://synbiohub.org/public/igem/BBa_K1088017/annotation2352406/range2352406/1
type: Range
persistent identity: range2352406
display id: range2352406
version: 1
top level: AraC
direction: direction/reverse
start: 51
end: 56
orientation: inline

BBa_K1088005
type: SequenceAnnotation
persistent identity: annotation2352408
display id: annotation2352408
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_K1088017/annotation2352408/range2352408/1
component: BBa_K1088005

https://synbiohub.org/public/igem/BBa_K1088017/annotation2352408/range2352408/1
type: Range
persistent identity: range2352408
display id: range2352408
version: 1
top level: AraC
direction: direction/reverse
start: 57
end: 935
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2352413
display id: annotation2352413
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_K1088017/annotation2352413/range2352413/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1088017/annotation2352413/range2352413/1
type: Range
persistent identity: range2352413
display id: range2352413
version: 1
top level: AraC
direction: direction/reverse
start: 936
end: 969
orientation: inline

Rubber Fuc
type: ComponentDefinition
persistent identity: BBa K1088024
display id: BBa_K1088024
version: 1
was generated by: iGEM to SBOL conversion
description: HRT2 prenyltransferase from Hevea Brasilianis (ara promoter without araC: arabinose inducible)
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Source is specified in components
owned by: james
owned by: myers
mutable description: This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The gene is expressed from the arabinose promoter (BBa_I13453), which is induceable with arabinose under glucose scarce conditions. Note that the regulater insn't a part of this part and thus no/very little transcription is expected.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: There is a 3xFLAG-tag after the HRT2 stop codon, and thus the 3xFLAG-tag isn't translated.
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1088021
component: BBa_I13453
component: BBa_K1088025
component: BBa_K1088003
component: BBa_J15001
sequence annotation: BBa_I13453
sequence annotation: BBa_K1088021
sequence annotation: BBa_J15001
sequence annotation: BBa_K1088003
sequence annotation: BBa_K1088025
sequence: https://synbiohub.org/public/igem/BBa_K1088024_sequence/1

BBa_K1088021
type: Component
persistent identity: component2373587
display id: component2373587
version: 1
top level: Rubber Fuc
definition: BBa_K1088021
access: public

BBa_I13453
type: Component
persistent identity: component2373586
display id: component2373586
version: 1
top level: Rubber Fuc
definition: BBa_I13453
access: public

BBa_K1088025
type: Component
persistent identity: component2373592
display id: component2373592
version: 1
top level: Rubber Fuc
definition: 3xFLAG
access: public

BBa_K1088003
type: Component
persistent identity: component2373591
display id: component2373591
version: 1
top level: Rubber Fuc
definition: HRT2
access: public

BBa_J15001
type: Component
persistent identity: component2373590
display id: component2373590
version: 1
top level: Rubber Fuc
definition: BBa_J15001
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2373586
display id: annotation2373586
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373586/range2373586/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373586/range2373586/1
type: Range
persistent identity: range2373586
display id: range2373586
version: 1
top level: Rubber Fuc
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_K1088021
type: SequenceAnnotation
persistent identity: annotation2373587
display id: annotation2373587
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373587/range2373587/1
component: BBa_K1088021

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373587/range2373587/1
type: Range
persistent identity: range2373587
display id: range2373587
version: 1
top level: Rubber Fuc
direction: direction/reverse
start: 131
end: 136
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2373590
display id: annotation2373590
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373590/range2373590/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373590/range2373590/1
type: Range
persistent identity: range2373590
display id: range2373590
version: 1
top level: Rubber Fuc
direction: direction/reverse
start: 137
end: 146
orientation: inline

BBa_K1088003
type: SequenceAnnotation
persistent identity: annotation2373591
display id: annotation2373591
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373591/range2373591/1
component: BBa_K1088003

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373591/range2373591/1
type: Range
persistent identity: range2373591
display id: range2373591
version: 1
top level: Rubber Fuc
direction: direction/reverse
start: 147
end: 1001
orientation: inline

BBa_K1088025
type: SequenceAnnotation
persistent identity: annotation2373592
display id: annotation2373592
version: 1
top level: Rubber Fuc
location: https://synbiohub.org/public/igem/BBa_K1088024/annotation2373592/range2373592/1
component: BBa_K1088025

https://synbiohub.org/public/igem/BBa_K1088024/annotation2373592/range2373592/1
type: Range
persistent identity: range2373592
display id: range2373592
version: 1
top level: Rubber Fuc
direction: direction/reverse
start: 1002
end: 1067
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_B1002
type: ComponentDefinition
persistent identity: BBa B1002
display id: BBa_B1002
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, small, %T~=85%)
created: 2006-08-29T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: antiquity
owned by: james
owned by: myers
mutable description: Artifical terminator, estimated %T~=85
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1002
sequence annotation: Poly A tail
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1002_sequence/1

B1002
type: SequenceAnnotation
persistent identity: annotation1898412
display id: annotation1898412
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1002/annotation1898412/range1898412/1
type: Range
persistent identity: range1898412
display id: range1898412
version: 1
top level: BBa_B1002
direction: direction/forward
start: 1
end: 34
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898415
display id: annotation1898415
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898415/range1898415/1
type: Range
persistent identity: range1898415
display id: range1898415
version: 1
top level: BBa_B1002
direction: direction/reverse
start: 4
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898413
display id: annotation1898413
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1002/annotation1898413/range1898413/1
type: Range
persistent identity: range1898413
display id: range1898413
version: 1
top level: BBa_B1002
direction: direction/forward
start: 10
end: 25
orientation: inline

Poly A tail
type: SequenceAnnotation
persistent identity: annotation1898414
display id: annotation1898414
version: 1
top level: BBa_B1002
location: https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1002/annotation1898414/range1898414/1
type: Range
persistent identity: range1898414
display id: range1898414
version: 1
top level: BBa_B1002
direction: direction/forward
start: 25
end: 31
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

HRT2
type: ComponentDefinition
persistent identity: BBa K1088003
display id: BBa_K1088003
version: 1
was generated by: iGEM to SBOL conversion
description: Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA
created: 2013-07-09T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: The gene is synthesized by http://www.genscript.com/
owned by: james
owned by: myers
mutable description: This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We optimized codons to fit the most used codons of E. coli.
star: false
creator: Andreas Kj??r, Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1088003_sequence/1

araC
type: ComponentDefinition
persistent identity: BBa K1088005
display id: BBa_K1088005
version: 1
was generated by: iGEM to SBOL conversion
description: arabinose regulator (araC) coding sequence
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: E. coli K-12 MG1655
owned by: james
owned by: myers
mutable description: This sequence is a copy of the sequence 70,387 -> 71,265 in E. coli K-12 MG1655 which is the coding sequence of the pBAD repressor araC. From ECOCYC: The "arabinose regulator," AraC, is a transcription factor that regulates transcription of several genes and operons involved in arabinose catabolism and transport. It coregulates with another transcriptional regulator, CRP; both are transcription factors involved in l-arabinose degradation. These regulators bind cooperatively to activate transcription of five operons related to transport, catabolism, and autoregulation of l-arabinose. Transcription of these operons is induced when E. coli is grown in the absence of glucose and when the physiological inducer, l-arabinose, binds to the AraC regulator. In the absence of glucose, cellular cyclic AMP levels are high and cyclic AMP forms a dimeric complex with CRP to coregulate with AraC. For more info go to http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10054
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part was amplified from chromosomal DNA with primers carrying prefix and suffix overhangs
star: false
creator: Andreas Kj??r
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: araC coding region
sequence: https://synbiohub.org/public/igem/BBa_K1088005_sequence/1

araC coding region
type: SequenceAnnotation
persistent identity: annotation2331909
display id: annotation2331909
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_K1088005/annotation2331909/range2331909/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1088005/annotation2331909/range2331909/1
type: Range
persistent identity: range2331909
display id: range2331909
version: 1
top level: araC
direction: direction/forward
start: 1
end: 879
orientation: inline

BBa_K1088021
type: ComponentDefinition
persistent identity: BBa K1088021
display id: BBa_K1088021
version: 1
was generated by: iGEM to SBOL conversion
description: Mixed site (ACTAGA)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Restriction sites
owned by: james
owned by: myers
mutable description: Mixed site (ACTAGA)when XbaI and SpeI sticky ends ligate
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088021_sequence/1

BBa_K1088022
type: ComponentDefinition
persistent identity: BBa K1088022
display id: BBa_K1088022
version: 1
was generated by: iGEM to SBOL conversion
description: TACTAG
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: TACTAG
owned by: james
owned by: myers
mutable description: Short DNA piece (TACTAG)
discontinued: false
dominant: false
group u list: _1398_
m user id: 0
owner id: 17057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 6 random nucleotides
star: false
creator: Patrick Rosendahl Andreassen
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1088022_sequence/1

3xFLAG
type: ComponentDefinition
persistent identity: BBa K1088025
display id: BBa_K1088025
version: 1
was generated by: iGEM to SBOL conversion
description: 3xFLAG
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:09:06Z
mutable provenance: Sigma Aldrich
owned by: james
owned by: myers
mutable description: 3xFLAG tag that can be fused with proteins either at the C- or N-terminal end. It can be used to purify the tagged protein or check levels of expression of tagged protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _1398_
m user id: 0
owner id: 12807
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Andreas Kj??r
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_K1088025_sequence/1

https://synbiohub.org/public/igem/BBa_K1088016_sequence/1
type: Sequence
persistent identity: BBa K1088016 sequence
display id: BBa_K1088016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataacgcaaaaaaccccgcttcggcggggttttttcgctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcactagactcaaggaggatggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataagactacaaagaccatgacggtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088017_sequence/1
type: Sequence
persistent identity: BBa K1088017 sequence
display id: BBa_K1088017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagcattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataacgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088024_sequence/1
type: Sequence
persistent identity: BBa K1088024 sequence
display id: BBa_K1088024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcactagactcaaggaggatggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataagactacaaagaccatgacggtgattataaagatcatgatatcgactacaaagatgacgacgataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1002_sequence/1
type: Sequence
persistent identity: BBa B1002 sequence
display id: BBa_B1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcttcggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088003_sequence/1
type: Sequence
persistent identity: BBa K1088003 sequence
display id: BBa_K1088003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaattatacaacggtgaaaggccgagcgtgttcagactgctggggaaatatatgcgtaaaggcctgtatagcatcctgacccagggtccgatcccgacccatattgccttcatcctggatggtaacggtcgttttgcgaaaaaacataaactgccggaaggtggtggtcataaagcgggttttctggcgctgctgaacgtgctgacctattgctatgaactgggcgtgaaatatgcgactatctatgcctttagcatcgataactttcgtcgtaaaccgcatgaagttcagtacgtgatgaacctgatgctggaaaaaattgaaggtatgatcatggaagaaagcatcatcaacgcatatgatatttgcgtgcgttttgttggtaacctgaaactgctggatgaaccgctgaaaaccgcagcagataaaattatgcgtgcgaccgccaaaaactccaaatttgtgctgctgctggcggtgtgctacacctcaaccgatgaaatcgtgcatgcggttgaagaatcctctaaagataaactgaaatccgatgaaatttgcaacgatggcaacggcgattgtgtgattaaaattgaagaaatggaaccgtattctgaaatcaaactggtggaactggaacgtaacacctacattaacccgtatccggatgtgctgattcgtacctctggcgaaacccgtctgagcaactacctgctgtggcagaccaccaactgcattctgtattctccgcatgcgctgtggccggaaattggtctgcgtcacgtggtgtgggcggtgattaactgccagcgtcattattcttacctggaaaaacataaagaatacctgaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088005_sequence/1
type: Sequence
persistent identity: BBa K1088005 sequence
display id: BBa_K1088005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088021_sequence/1
type: Sequence
persistent identity: BBa K1088021 sequence
display id: BBa_K1088021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088022_sequence/1
type: Sequence
persistent identity: BBa K1088022 sequence
display id: BBa_K1088022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1088025_sequence/1
type: Sequence
persistent identity: BBa K1088025 sequence
display id: BBa_K1088025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gactacaaagaccatgacggtgattataaagatcatgatatcgactacaaagatgacgacgataaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z