LOCUS BBa_K1088019 1173 bp DNA linear 25-Nov-2024 DEFINITION LacI device (constitutive promoter, RBS and terminator) ACCESSION BBa_K1088019 VERSION BBa_K1088019.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 36..50 /label=RBS-1\Strong RBS 43..47 /label=RBS misc_feature 36..50 /label=BBa_B0030 RBS 36..50 /label=BBa_B0030 misc_feature 51..56 /label=BBa_K1088022 CDS 57..1139 /label=BBa_K1088018 misc_feature 1140..1173 /label=B1002 stem_loop 1149..1164 /label=stem loop polyA_site 1164..1170 /label=Poly A tail polyA_site 1143..1148 /label=Poly A tail terminator 1140..1173 /label=BBa_B1002 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatga 61 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 121 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 181 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 241 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 301 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 361 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 421 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 481 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 541 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 601 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 661 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 721 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 781 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 841 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 901 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 961 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 1021 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 1081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgac 1141 gcaaaaaacc ccgcttcggc ggggtttttt cgc //