LOCUS BBa_K1088026 4049 bp DNA linear 22-Nov-2024 DEFINITION B. subtilis dxs-GFP protein fusion (lac promoter with lac inhibitor: IPTG inducible) ACCESSION BBa_K1088026 VERSION BBa_K1088026.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 36..50 /label=RBS-1\Strong RBS 43..47 /label=RBS misc_feature 36..50 /label=BBa_B0030 RBS 36..50 /label=BBa_B0030 misc_feature 51..56 /label=BBa_K1088022 CDS 57..1139 /label=BBa_K1088018 misc_feature 1140..1173 /label=B1002 stem_loop 1149..1164 /label=stem loop polyA_site 1164..1170 /label=Poly A tail polyA_site 1143..1148 /label=Poly A tail terminator 1140..1173 /label=BBa_B1002 promoter 1..1173 /label=BBa_K1088019 CDS 1182..1269 /label=end of LacI coding region (inactive) misc_feature 1182..1381 /label=BBa_R0010 misc_feature 1270..1307 /label=CAP binding site promoter 1318..1323 /label=-35 promoter 1342..1347 /label=-10 misc_feature 1347..1381 /label=LacI binding site misc_feature 1354..1354 /label=start promoter 1182..1381 /label=BBa_R0010 misc_feature 1382..1387 /label=BBa_K1088021 misc_feature 1388..1390 /label=SacI RBS 1391..1397 /label=rbs RBS 1388..1397 /label=BBa_J15001 CDS 1398..3298 /label=Dxs B. sub coding region CDS 1398..3299 /label=BBa_K1088000 misc_feature 3300..3329 /label=BBa_K105012 CDS 3330..4049 /label=GFP protein CDS 3330..4049 /label=BBa_E0040 misc_feature 1182..4049 /label=BBa_K1088008 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatga 61 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 121 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 181 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 241 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 301 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 361 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 421 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 481 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 541 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 601 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 661 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 721 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 781 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 841 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 901 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 961 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 1021 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 1081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgac 1141 gcaaaaaacc ccgcttcggc ggggtttttt cgctactaga gcaatacgca aaccgcctct 1201 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1261 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 1321 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 1381 aactagactc aaggaggatg gatcttttat caatacagga cccgtcgttt ttaaaaaaca 1441 tgtccattga tgaattagag aaattaagtg atgaaatccg tcagttttta attacaagtt 1501 tatccgcttc cggcggccac atcggcccaa acttaggtgt cgtagagctt actgttgccc 1561 tgcataagga atttaacagc ccgaaagaca aatttttatg ggatgtaggc catcagtcgt 1621 atgtccataa gctgctgaca ggacgcggaa aagaatttgc gacgcttcgc cagtacaaag 1681 ggctttgcgg atttccaaag cggagtgaaa gcgagcacga tgtttgggaa accgggcaca 1741 gctcgacttc tctgtcaggc gcgatgggaa tggcagctgc ccgtgatatt aaaggaacgg 1801 atgaatatat tattccgatc attggtgacg gcgcgctgac cggcggtatg gcgctcgaag 1861 cccttaacca catcggcgac gagaaaaaag acatgattgt catccttaat gataatgaaa 1921 tgagtattgc gccaaacgtc ggtgccattc actctatgct cggacggctc cgcactgcgg 1981 ggaaatacca gtgggtcaaa gatgagcttg aatacttatt taaaaagatt ccggcagttg 2041 ggggcaagct tgccgccacg gcggaacggg tcaaagacag cctgaaatac atgctcgtct 2101 ccggaatgtt tttcgaggag ctcggtttta cgtatttggg cccagtggac ggacattctt 2161 atcatgagct gattgagaat cttcaatacg ccaaaaaaac gaaaggccct gttcttctgc 2221 acgtcattac gaaaaaaggg aaggggtaca aaccggctga gaccgatacg attgggacat 2281 ggcatggtac cggaccatat aaaattaata ccggtgactt tgtaaagccg aaagccgcag 2341 ctccttcgtg gagcggtctt gtcagcggaa ctgtgcagcg aatggcgcgc gaggacggac 2401 gcattgtagc cattacgccg gctatgcctg tcggttcaaa gcttgaaggc ttcgcaaagg 2461 aattccctga ccggatgttc gacgtaggaa tcgcagaaca gcatgccgca acaatggctg 2521 cagctatggc aatgcagggt atgaagccgt ttttggcgat ttactcaacc ttcctgcaaa 2581 gggcatatga ccaagttgtt catgacatct gccgccaaaa cgctaatgtg tttattggaa 2641 ttgaccgtgc tggactcgtt ggcgctgatg gagagacaca tcaaggcgtg tttgatattg 2701 cgtttatgcg ccacattcca aacatggtct taatgatgcc gaaagacgaa aatgaaggcc 2761 agcacatggt tcatacagca cttagctatg acgaaggccc gatagcaatg cgttttccgc 2821 gcggaaacgg actcggcgta aaaatggatg aacagttgaa aacgattccg atcggtacgt 2881 gggaggtgct gcgtccaggg aacgatgctg tcatcttaac attcggcaca acaatcgaaa 2941 tggcgattga agcagccgaa gagctgcaga aagaaggcct ttccgtgcgc gttgtgaatg 3001 cgcgttttat taagccgatt gatgaaaaga tgatgaagag tatcctaaaa gaaggcttgc 3061 caattttaac aattgaagaa gcggtcttag aaggcggttt cggaagctcg attttagaat 3121 tcgctcatga tcaaggtgaa tatcatactc cgattgacag aatgggtata cctgatcggt 3181 ttattgaaca cggaagtgta acagcgcttc ttgaggaaat tggactgaca aaacagcagg 3241 tggcaaatcg tattagatta ctgatgccac caaagacaca caaaggaatt ggatcatgag 3301 gtgaaaattt gtattttcaa tctggtggta tgcgtaaagg agaagaactt ttcactggag 3361 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 3421 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 3481 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 3541 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 3601 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 3661 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 3721 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 3781 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 3841 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 3901 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 3961 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 4021 atggcatgga tgaactatac aaataataa //