LOCUS       BBa_K1088052             887 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP reporter with flexible linker at N-terminus for creation of 
            GFP fusions
ACCESSION   BBa_K1088052
VERSION     BBa_K1088052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=BamHI-site
     CDS             6..24
                     /label=TEV recognition-site
     misc_feature    1..30
                     /label=BBa_K1088051
     CDS             31..750
                     /label=GFP protein
     CDS             31..750
                     /label=BBa_E0040
     stem_loop       770..813
                     /label=stem_loop
     misc_feature    759..838
                     /label=BBa_B0010
     terminator      759..838
                     /label=BBa_B0010
     stem_loop       854..873
                     /label=T7 TE
     misc_feature    880..880
                     /label=stop
     polyA_site      874..887
                     /label=polya
     misc_feature    847..887
                     /label=BBa_B0012
     terminator      847..887
                     /label=BBa_B0012
     misc_feature    31..887
                     /label=BBa_I13401
ORIGIN
        1 ggatccgaaa atttgtattt tcaatctggt atgcgtaaag gagaagaact tttcactgga
       61 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      121 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      181 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      241 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      301 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      361 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      421 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      481 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      541 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      601 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      661 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      721 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
      781 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      841 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//