LOCUS       BBa_K1088057             848 bp    DNA     linear       15-Jun-2024
DEFINITION  T25 domain of bacterial two-hybrid system (IPTG inducible)
ACCESSION   BBa_K1088057
VERSION     BBa_K1088057.1  
FEATURES             Location/Qualifiers
     promoter        88..118
                     /label=Lac promoter
     misc_feature    52..73
                     /label=CAP binding
     misc_feature    126..142
                     /label=Lac operator
     promoter        1..142
                     /label=BBa_K1088054
     RBS             150..156
                     /label=Shine Dalgarno
     RBS             143..161
                     /label=BBa_K1088055
     misc_feature    843..848
                     /label=BamHI restriction site
     CDS             162..848
                     /label=BBa_K1088056
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg cagcaatcgc
      181 atcaggctgg ttacgcaaac gccgccgacc gggagtctgg catccccgca gccgtactcg
      241 atggcatcaa ggccgtggcg aaggaaaaaa acgccacatt gatgttccgc ctggtcaacc
      301 cccattccac cagcctgatt gccgaagggg tggccaccaa aggattgggc gtgcacgcca
      361 agtcgtccga ttgggggttg caggcgggct acattcccgt caacccgaat ctttccaaac
      421 tgttcggccg tgcgcccgag gtgatcgcgc gggccgacaa cgacgtcaac agcagcctgg
      481 cgcatggcca taccgcggtc gacctgacgc tgtcgaaaga gcggcttgac tatctgcggc
      541 aagcgggcct ggtcaccggc atggccgatg gcgtggtcgc gagcaaccac gcaggctacg
      601 agcagttcga gtttcgcgtg aaggaaacct cggacgggcg ctatgccgtg cagtatcgcc
      661 gcaagggcgg cgacgatttc gaggcggtca aggtgatcgg caatgccgcc ggtattccac
      721 tgacggcgga tatcgacatg ttcgccatta tgccgcatct gtccaacttc cgcgactcgg
      781 cgcgcagttc ggtgaccagc ggcgattcgg tgaccgatta cctggcgcgc acgcggcggg
      841 ctggatcc
//