BBa_K1088055 1 pKT25 RBS RBS from pKT25 2015-03-29T11:00:00Z 2015-05-08T01:09:07Z pKT25 Ribosomal binding site from pKT25 false false _1398_ 0 17057 9 Not in stock false No false Patrick Rosendahl Andreassen annotation2430673 1 Shine Dalgarno range2430673 1 8 14 BBa_K1088057 1 BBa_K1088057 T25 domain of bacterial two-hybrid system (IPTG inducible) 2015-03-29T11:00:00Z 2015-05-08T01:09:07Z pKT25 zsdf false false _1398_ 0 17057 9 Not in stock false YES!! false Patrick Rosendahl Andreassen component2430686 1 BBa_K1088054 component2430690 1 BBa_K1088056 component2430688 1 BBa_K1088055 annotation2430686 1 BBa_K1088054 range2430686 1 1 142 annotation2430690 1 BBa_K1088056 range2430690 1 162 848 annotation2430688 1 BBa_K1088055 range2430688 1 143 161 BBa_K1088054 1 pLac Lac operator 2015-03-29T11:00:00Z 2015-05-08T01:09:06Z pKT25 This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein. In the absence of LacI protein and CAP protein, this part promotes transcription. In the presence of LacI protein and CAP protein, this part inhibits transcription. LacI can be inhibited by IPTG. LacI is coded by BBa_C0010 false false _1398_ 0 17057 9 Not in stock false No false Patrick Rosendahl Andreassen annotation2430670 1 Lac promoter range2430670 1 88 118 annotation2430672 1 Lac operator range2430672 1 126 142 annotation2430671 1 CAP binding range2430671 1 52 73 BBa_K1088056 1 T25 domain T25 domain of CyaA from Bordetella pertussis 2015-03-29T11:00:00Z 2015-05-08T01:09:07Z pKT25 hj false false _1398_ 0 17057 9 Not in stock false YES!! false Patrick Rosendahl Andreassen annotation2430674 1 BamHI restriction site range2430674 1 682 687 BBa_K1088054_sequence 1 ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaa BBa_K1088055_sequence 1 tttcacacaggaaacagct BBa_K1088056_sequence 1 atgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc BBa_K1088057_sequence 1 ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgcagcaatcgcatcaggctggttacgcaaacgccgccgaccgggagtctggcatccccgcagccgtactcgatggcatcaaggccgtggcgaaggaaaaaaacgccacattgatgttccgcctggtcaacccccattccaccagcctgattgccgaaggggtggccaccaaaggattgggcgtgcacgccaagtcgtccgattgggggttgcaggcgggctacattcccgtcaacccgaatctttccaaactgttcggccgtgcgcccgaggtgatcgcgcgggccgacaacgacgtcaacagcagcctggcgcatggccataccgcggtcgacctgacgctgtcgaaagagcggcttgactatctgcggcaagcgggcctggtcaccggcatggccgatggcgtggtcgcgagcaaccacgcaggctacgagcagttcgagtttcgcgtgaaggaaacctcggacgggcgctatgccgtgcagtatcgccgcaagggcggcgacgatttcgaggcggtcaaggtgatcggcaatgccgccggtattccactgacggcggatatcgacatgttcgccattatgccgcatctgtccaacttccgcgactcggcgcgcagttcggtgaccagcggcgattcggtgaccgattacctggcgcgcacgcggcgggctggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z