LOCUS       BBa_K1088059             916 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP reporter with flexible linker at N-terminus for creation of 
            GFP fusions
ACCESSION   BBa_K1088059
VERSION     BBa_K1088059.1  
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /label=BBa_K1088058
     RBS             39..53
                     /label=RBS-1\Strong
     RBS             46..50
                     /label=RBS
     misc_feature    39..53
                     /label=BBa_B0030
     RBS             39..53
                     /label=BBa_B0030
     CDS             60..779
                     /label=GFP protein
     CDS             60..779
                     /label=BBa_E0040
     stem_loop       799..842
                     /label=stem_loop
     misc_feature    788..867
                     /label=BBa_B0010
     terminator      788..867
                     /label=BBa_B0010
     stem_loop       883..902
                     /label=T7 TE
     misc_feature    909..909
                     /label=stop
     polyA_site      903..916
                     /label=polya
     misc_feature    876..916
                     /label=BBa_B0012
     terminator      876..916
                     /label=BBa_B0012
     misc_feature    39..916
                     /label=BBa_E0840
ORIGIN
        1 ggatccgaaa atttgtattt tcaatctggt tactagagat taaagaggag aaatactaga
       61 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      121 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      181 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      241 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      301 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      361 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      421 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      481 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      541 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      601 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      661 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      721 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
      781 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      901 cctttctgcg tttata
//