LOCUS BBa_K1088061 4789 bp DNA linear 22-Dec-2024 DEFINITION Strong Promoter + Strong RBS + I-E Cascade ACCESSION BBa_K1088061 VERSION BBa_K1088061.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23104 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1..55 /label=BBa_K608002 CDS 64..2063 /label=Prot 1 CDS 64..4789 /label=BBa_K1088060 ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gagccatggt ctcacatgag tcggtttaat ttacttgatg aaccctggat ttctgttatt 121 tatgacgaga aaggatccac aaaagatgtt tctttacagg atttatttac aaatgctcat 181 cagtataaag aattagcagg agatacgaaa acacaagatt ttgcggtatt acgagtattg 241 ttggctgttt tgcatacagt tttttcccga tttgatatag atggaaatgc ttatgagtat 301 cttactattg atgaaggatg gaatcagctt gaaccagtgg atgagatgga tattgagaat 361 tatgaagaag ctctttatga aacatgggaa aaactttgga ctaataaaag atttccaaat 421 attgttaatc aatacttaga aaaatggcga gatcgctttt acttatttga ccaaaagtat 481 ccattttttc aggtaacgaa agaagatatt gctggagata aaattagcaa agccaaagga 541 actagtattt tgggtaaaaa tataaataga attatatctg agagtggaaa taagatagct 601 ttattttccc caaaagatga agaaaataaa aatactctaa ctgccgcaga attagctaga 661 tggctaatta catttcaagg gtattcagga gtatccgata aggtcatttt tggaaatgaa 721 aaatatacac cttcgaaagg atggctattt gatattggtg ctatttatat taaaggtaac 781 actttattcg aaacgttgtt actaaattat atttcaccgt ataatgaatg tggtaatgta 841 gaaaatattc agcgaccttg ctgggaacgt aaaagttctg atatcattaa aagttacctt 901 gatgaaaaag atattaccaa tatagctagt ttatatactg tttggagtaa aggaatctat 961 attgacccag actttaattt aaataaacct ttctcttttg atattgtgaa attacctgat 1021 atcaatcata gggataattt tttggagcca atgacacttt ggaagtataa tgtttcgggt 1081 aaaaataggg atagctatac accaaagaaa catcttttga atcagtcttt atggcgatct 1141 tttggcttgt tatctataaa agatactgat cttcaacata gaaaacctgg cgttatcgaa 1201 tggcttacat atattgatga tataatagga aatcgactat caaacattgt tgcaattagt 1261 atgcaggacg atggaaaccc tcaatcacgg cttccaacgg atgaggttat tgactcaatt 1321 tttatcaatg atttggtttt aacggattta gacgagggtg gttgggttcc tcgtatcaat 1381 gaagtagtag aagaaacaaa aaaaattatt tctagaacat ataaaacgta tattaatgat 1441 ataaaggaaa tcagaaatat ctctaatggt agttatactc agcagatagt tgaaagtcta 1501 tatttcaaaa tcgatcaacc tttccgacaa tggttagcta gcattcaacc agaagatgat 1561 aaagattcta agatacaaga atggagagtt ttactgaaga aaatagttaa agctgaagca 1621 gaagttattt tacatcaagg gggggcgagg gattatcttg gtatccaaaa agatgggcga 1681 attaagaata ttgcaactgc ctataattcg ttcgattttt ggcttcgtca acagttaaaa 1741 taggagcaga gaatgtcaca acatactaaa caaacagttt ggactgttac aaagaagatt 1801 attttcaaat tgagtaacca aaaagaggaa acatatgata aagctacact agcaaaagtt 1861 agaaaaacat tggggaaacc cttgagtgaa gcaacagaaa tatggccaat tctgttcgaa 1921 aatttaccag aggaattttt aagttcgtat cagcaaccaa gctatgagga attagctatt 1981 tatacagcgc ttcaattgta tgcattgcat cagcaaggtg tttcaacaag tgtgatgcta 2041 gatgattcta agccctatga aaatataggt tctgcacttg gtaagcttag gaaagaagat 2101 gataccactt caattgatag acgattcaat accatgatta cctcatcaac gtttgaagaa 2161 cttacttatc atttaaggca tttgatttcg ctacttaaag tggagtcacc ggtgattcag 2221 gttgattact ctggcctagc aaatgattta tattggtttt taaataatag tcaagaaaat 2281 gtccgcctaa attgggcaag agcatattat aaacaaatta aaggagaaaa aaacaatgac 2341 aactgaacaa cgattatttt tagatattca tgctattcaa agcttaccac cttcaaatat 2401 gaatagagat gatacaggta gcccgaaaac agctcagtat ggaggagtta gaagatctcg 2461 cgtaagttct caagcatgga agaaagcgat gagagagtat tttaatactc atggtgatca 2521 ttcaaatgtt ggtattagaa caaaagaaat tgtgagatat gttgcagata aaatagtaga 2581 actagatagt tctatcagta ttgaagatgc tttggaaaaa gccgataaag tcttgatagc 2641 tgctggaatc aagaaaaaag gtgaagttaa agcactttat tttatgggtg atagtcaagc 2701 taagaagtta gctcaagcag cctatgacaa tataacacat aaaaaagagc ttcaaaagtt 2761 ggctaatgaa gatccagcta ttgatattgc cctgtttggt cgaatggtag ccgaagatcc 2821 aactcttaac gaggattctt cttctcaggt tgcacatgcc atttccacac atccagtaca 2881 aactgaattt gatttcttta cggctgttga tgatttagct ccagaggata atgcaggggc 2941 gggtatgctt ggaactgttg agtttaattc ttctacacta tatcgctatg caaatgttgc 3001 gattcatgaa ttgtcaaaac aattaggaaa caaggaatcg ttggagaata gtttatcttt 3061 gttcattgag gcttttgcaa agtcacttcc aacagggaaa gtaaatacat ttgctaatca 3121 aacgctacca cagactttaa ttgtaacggt tcgaagtgat cgtccggtta atcttgtcag 3181 tgcatttgaa gaaccagtta aatcaataga aggctatgtt actaaatcta tcgataaact 3241 cgccaaagag tttattaagg ttgagaaatt tgtagacaaa ccacttatga ccttctatgt 3301 tactttgcat gagagcgaca gtctaaaaca aattggggag gaaaaagatt cagtttcaaa 3361 attattggaa gatttttcag aaatgatagc ccaatatatt taaaaaggtg aatgctatga 3421 agacgatatt gttgaaattt gctggcccct tacaatcatg gggaacgagt tcacattttg 3481 aaactaggca tacagatttt tatccttcca aaagtgcggt aattggacta cttgctgcta 3541 gtttaggtta taggagagac gaagatgaaa aaattcaaaa attaaatgaa cttgattttg 3601 ctgttcgtgt cgatcagcaa ggaaatctgt taagagactt ccatgttgca cagaagtata 3661 cagaaaaggg taaatttgat agaaattatg ttacgaatcg gtattatcta gaagacgctg 3721 tatttgttgt agccctctcc catacagatg atagctttat agatatcatt aaaggtgctc 3781 taaaaaatcc ctatttccaa ccttttatgg ggagaagggc actcccaata actgcagact 3841 ttatcttagg tgagacagat gttaatccat tagagagcct aaaatcgctg aaatggcaag 3901 cttctctatg gtttatgaaa aaatctcatg ataaggatgc tatttctttg gaagtttatc 3961 tagatagtca attagtcaac aaggatgttt atcagctgag gcaagattat gttaggtcat 4021 tttcacaaaa agggagaaga tttggtttta ggtatgaagt acgtgaatta atcagtgttc 4081 aaaatcctca ctatttaaat aagaatacag agcataacat tttcgagagt ataggagatt 4141 gaagtaatgt acatttctag ggtagaaatt gatcgatata atagacgaaa ggttagagat 4201 ttaacgcatg taggagctta tcatgcttgg gtggaagaga gttttccatc ggaattagag 4261 caatcaatcc gtacaagaaa attatggcga atcgatcgta ttcaaggtaa ggattatctt 4321 attattgtaa gtaaagaaaa acctaattta caaaaacttg aaaaatatgg tgttgtagga 4381 agcgctcaaa ctaaggacta tcaacatttt ttagatagca tcaaaactgg ttttcgaatg 4441 aattttagaa tagtgctaaa tccggttatt tcaattactt cacccgataa atcaaaacga 4501 ggagttgtaa aaccgcatgt gacgattaaa catcagatgg actttctagt gaaacaatct 4561 gagaaaaatg gcttctcatt agtaggagaa agtttctcaa ttgttgaacg tggttatgaa 4621 gtttttcaga aaccagaaaa acccatacga ctatcaaaag ttgtttacga aggaacctta 4681 actgttagtg atgatacgct tttcaaaaaa atgctgaccg agggagttgg aaagaaaaaa 4741 gcatatggtt ttggcttgat gacagtggta ccacttgagg aactcgagt //