LOCUS       BBa_K1090000            1738 bp    DNA     linear       22-Nov-2024
DEFINITION  AHL signal sender with RFP reporter under lac promoter control
ACCESSION   BBa_K1090000
VERSION     BBa_K1090000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    806..837
                     /label=LVA
     CDS             227..805
                     /label=luxI
     misc_feature    227..844
                     /label=BBa_C0061
     misc_feature    845..869
                     /label=Help:Barcodes
     CDS             227..844
                     /label=BBa_C0061
     misc_feature    209..869
                     /label=BBa_C0261
     misc_feature    882..885
                     /label=conserved
     RBS             878..889
                     /label=BBa_B0034
     CDS             896..1570
                     /label=mrfp1
     misc_feature    1577..1601
                     /label=Help:Barcodes
     CDS             896..1576
                     /label=BBa_E1010
     stem_loop       1621..1664
                     /label=stem_loop
     misc_feature    1610..1689
                     /label=BBa_B0010
     terminator      1610..1689
                     /label=BBa_B0010
     stem_loop       1705..1724
                     /label=T7 TE
     misc_feature    1731..1731
                     /label=stop
     polyA_site      1725..1738
                     /label=polya
     misc_feature    1698..1738
                     /label=BBa_B0012
     terminator      1698..1738
                     /label=BBa_B0012
     misc_feature    878..1738
                     /label=BBa_I13507
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta
      841 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatggc
      901 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      961 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1021 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1081 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1141 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1201 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1261 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1321 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1381 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1441 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1501 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1561 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1621 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1681 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//