LOCUS       BBa_K109201             1016 bp    DNA     linear       21-May-2024
DEFINITION  GFP under AraC and TetR (K109200 + E0240)
ACCESSION   BBa_K109201
VERSION     BBa_K109201.1  
FEATURES             Location/Qualifiers
     misc_feature    34..50
                     /label=AraC
     misc_feature    81..99
                     /label=TetR
     promoter        49..54
                     /label=-35
     promoter        73..78
                     /label=-10
     promoter        1..132
                     /label=BBa_K109200
     RBS             141..153
                     /label=RBS-3\Weak
     RBS             147..150
                     /label=RBS
     misc_feature    141..153
                     /label=BBa_B0032
     RBS             141..153
                     /label=BBa_B0032
     CDS             160..879
                     /label=GFP protein
     CDS             160..879
                     /label=BBa_E0040
     stem_loop       899..942
                     /label=stem_loop
     misc_feature    888..967
                     /label=BBa_B0010
     terminator      888..967
                     /label=BBa_B0010
     stem_loop       983..1002
                     /label=T7 TE
     misc_feature    1009..1009
                     /label=stop
     polyA_site      1003..1016
                     /label=polya
     misc_feature    976..1016
                     /label=BBa_B0012
     terminator      976..1016
                     /label=BBa_B0012
ORIGIN
        1 cacctcgagt acaacgtcgt gttagctgcc ttttagcaat tttatccata gacacctgta
       61 ggatcgtaca ggtataatta actctatcaa tgatagagtg tcaacaaaaa aacggatcct
      121 ctagttgcgg cctactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt
      181 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      241 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      301 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      361 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      421 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      481 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      541 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      601 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      661 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
      721 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
      781 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
      841 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
      901 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      961 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//