##gff-version 3
##sequence-region BBa_K109202 1 1076
BBa_K109202	.	engineered_region	141	1076	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_K109202	.	CDS	159	914	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K109202	.	engineered_region	159	160	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K109202	.	engineered_tag	915	939	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K109202	.	CDS	159	908	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K109202	.	point_mutation	650	650	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K109202	.	point_mutation	332	332	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K109202	.	engineered_region	159	914	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K109202	.	terminator	1036	1076	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K109202	.	stem_loop	1043	1062	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K109202	.	polyA_site	1063	1076	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K109202	.	stop_codon	1069	1069	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K109202	.	engineered_region	1036	1076	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K109202	.	ribosome_entry_site	141	152	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K109202	.	conserved_region	145	148	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K109202	.	terminator	948	1027	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K109202	.	stem_loop	959	1002	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K109202	.	engineered_region	948	1027	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K109202	.	promoter	1	132	.	+	0	ID=BBa_K109200;Name=BBa_K109200
BBa_K109202	.	promoter	73	78	.	+	0	ID=annotation1980471;Name=-10;Parent=BBa_K109200
BBa_K109202	.	non_covalent_binding_site	81	99	.	+	0	ID=annotation1969066;Name=TetR;Parent=BBa_K109200
BBa_K109202	.	non_covalent_binding_site	34	50	.	+	0	ID=annotation1969061;Name=AraC;Parent=BBa_K109200
BBa_K109202	.	promoter	49	54	.	+	0	ID=annotation1980470;Name=-35;Parent=BBa_K109200
>BBa_K109202
cacctcgagtacaacgtcgtgttagctgccttttagcaattttatccatagacacctgtaggatcgtacaggtataatt
aactctatcaatgatagagtgtcaacaaaaaaacggatcctctagttgcggcctactagagaaagaggagaaatactag
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata