LOCUS       BBa_K1092020            1892 bp    DNA     linear       21-May-2024
DEFINITION  T7-RBS-ssDsbA-PDZ Ligand-FL3 Lgt FL3-Laccase
ACCESSION   BBa_K1092020
VERSION     BBa_K1092020.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..116
                     /label=BBa_K1092101
     misc_feature    145..156
                     /label=B0034
     promoter        125..144
                     /label=T7 promoter
     RBS             148..151
                     /label=RBS
     promoter        125..156
                     /label=BBa_K525998
     misc_feature    165..344
                     /label=BBa_K1092006
     misc_feature    125..344
                     /label=BBa_K1092013
     CDS             351..1892
                     /label=BBa_K1092004
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaaaagatt tggctggcgc
       61 tggctggttt agttttagcg tttagcgcat cggcgggcgt gaaggaatct ctggtgtact
      121 agagtaatac gactcactat agggaaagag gagaaatact agagggtggt ggtggttctg
      181 gtggtggtgg ttctggtggt ggtggttctc tgggtctgtt tcgtctggtg cgcctgctgc
      241 gttttctgcg tattctgctg attatttgcc cggaaccgga accggaacca gaaccagaag
      301 gtggtggtgg ttctggtggt ggtggttctg gtggtggtgg ttcttactag atgacacttg
      361 aaaaatttgt ggatgctctc ccaatcccag atacactaaa gccagtacag caatcaaaag
      421 aaaaaacata ctacgaagtc accatggagg aatgcaccca tcagctccat cgcgatctcc
      481 ctccaacccg cctgtggggc tacaacggct tatttccggg gccgaccatt gaggttaaaa
      541 gaaatgaaaa cgtatatgta aaatggatga ataacctacc ttccacgcat ttccttccga
      601 ttgatcacac cattcatcac agtgacagcc agcatgaaga gtccgaggta aagactgttg
      661 ttcatttaca cggcggcgtc acgccagatg acagtgacgg gtatcccgag gcttggtttt
      721 ccaaagactt tgaacaaaca ggaccttatt tcaaaagaga ggtttatcat tatccaaacc
      781 agcagcgcgg ggctatattg tggtatcacg atcacgccat ggcgctcacc aggctaaatg
      841 tctatgccgg acttgtcggt gcttatatca ttcatgaccc aaaggaaaaa cgcttaaaac
      901 tgccttcaga cgaatacgat gtgccgcttc ttatcacaga ccgcacgatc aatgaggatg
      961 gttctttgtt ttatccgagc gcaccggaaa acccttctcc atcactgcct aatccttcaa
     1021 tcgttccggc tttttgcgga gaaaccatac tcgtcaacgg gaaggtatgg ccatacttgg
     1081 aggtcgagcc gaggaaatac cgattccgtg tcatcaacgc ctccaataca agaacctata
     1141 acctgtcact cgataatggc ggagagttta ttcaggttgg ttcagatgga gggctcctgc
     1201 cgcgatctgt taaactgaac tctttcagcc ttgcgcctgc tgaacgttat gatatcatca
     1261 ttgacttcac agcatatgaa ggagaatcga tcattttggc aaacagcgcg ggctgcggcg
     1321 gtgacgtcaa tcctgaaaca gatgcgaata tcatgcaatt cagagtcaca aaaccattgg
     1381 cacaaaaaga cgaaagcaga aagccggagt acctcgcctc atacccttcg gtacagcatg
     1441 agagaataca aaacatcaga acgttaaaac tggcaggcac ccaggacgaa tacggcagac
     1501 ccgtccttct gcttaataac aaacgctggc acgatcccgt cacaggagca ccaaaagtcg
     1561 gcacaactga aatatggtcc attatcaacc cgacacgcgg aacacatccg atccacctgc
     1621 atctagtctc cttccgcgta ttagaccggc ggccgtttga tatcgcccgt tatcaagaaa
     1681 gcggggaatt gtcctatacc ggtccggctg tcccgccgcc gccaagtgaa gagggctgga
     1741 aagacaccat tcaagcgcat gcaggtgaag tcctgagaat cgcggcgaca ttcggtccgt
     1801 acagcggacg atacgtatgg cattgccata ttctggagca tgaagactat gacatgatga
     1861 gaccgatgga tataactgat cgccataaat aa
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