LOCUS       BBa_K1092025            2641 bp    DNA     linear       22-Nov-2024
DEFINITION  T7-RBS -ssDsbA-PDZ Ligand-Voltage Switch-Laccase
ACCESSION   BBa_K1092025
VERSION     BBa_K1092025.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..116
                     /label=BBa_K1092101
     misc_feature    125..1093
                     /label=BBa_K1092103
     CDS             1100..2641
                     /label=BBa_K1092004
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaaaagatt tggctggcgc
       61 tggctggttt agttttagcg tttagcgcat cggcgggcgt gaaggaatct ctggtgtact
      121 agaggctgag gccgccgcaa aagaagcagc agctaaggaa gctgcggcga agatgaccag
      181 tagctatctg cattttccgg agtttgatcc ggtcattttc tcaataggac ccgtggcgct
      241 tcactggtac ggcctgatgt atctggtggg tttcattttt gcaatgtggc tggcaacacg
      301 acgggcgaat cgtccgggca gcggctggac caaaaatgaa gttgaaaact tactctatgc
      361 gggcttcctc ggcgtcttcc tcgggggacg tattggttat gttctgttct acaatttccc
      421 gcagtttatg gccgatccgc tgtatctgtt ccgtgtctgg gacggcggca tgtctttcca
      481 cggcggcctg attggcgtta tcgtggtgat gattatcttc gcccgccgta ctaaacgttc
      541 cttcttccag gtctctgatt ttatcgcacc actcattccg tttggtcttg gtgccgggcg
      601 tctgggcaac tttattaacg gtgaattgtg gggccgcgtt gacccgaact tcccgtttgc
      661 catgctgttc cctggctccc gtacagaaga tattttgctg ctgcaaacca acccgcagtg
      721 gcaatccatt ttcgacactt acggtgtgct gccgcgccac ccatcacagc tttacgagct
      781 gctgctggaa ggtgtggtgc tgtttattat cctcaacctg tatattcgta aaccacgccc
      841 aatgggagct gtctcaggtt tgttcctgat tggttacggc gcgtttcgca tcattgttga
      901 gtttttccgc cagcccgacg cgcagtttac cggtgcctgg gtgcagtaca tcagcatggg
      961 gcaaattctt tccatcccga tgattgtcgc gggtgtgatc atgatggtct gggcatatcg
     1021 tcgcagccca cagcaacagg tttccgctga ggccgccgca aaagaagcag cagctaagga
     1081 agctgcggcg aagtactaga tgacacttga aaaatttgtg gatgctctcc caatcccaga
     1141 tacactaaag ccagtacagc aatcaaaaga aaaaacatac tacgaagtca ccatggagga
     1201 atgcacccat cagctccatc gcgatctccc tccaacccgc ctgtggggct acaacggctt
     1261 atttccgggg ccgaccattg aggttaaaag aaatgaaaac gtatatgtaa aatggatgaa
     1321 taacctacct tccacgcatt tccttccgat tgatcacacc attcatcaca gtgacagcca
     1381 gcatgaagag tccgaggtaa agactgttgt tcatttacac ggcggcgtca cgccagatga
     1441 cagtgacggg tatcccgagg cttggttttc caaagacttt gaacaaacag gaccttattt
     1501 caaaagagag gtttatcatt atccaaacca gcagcgcggg gctatattgt ggtatcacga
     1561 tcacgccatg gcgctcacca ggctaaatgt ctatgccgga cttgtcggtg cttatatcat
     1621 tcatgaccca aaggaaaaac gcttaaaact gccttcagac gaatacgatg tgccgcttct
     1681 tatcacagac cgcacgatca atgaggatgg ttctttgttt tatccgagcg caccggaaaa
     1741 cccttctcca tcactgccta atccttcaat cgttccggct ttttgcggag aaaccatact
     1801 cgtcaacggg aaggtatggc catacttgga ggtcgagccg aggaaatacc gattccgtgt
     1861 catcaacgcc tccaatacaa gaacctataa cctgtcactc gataatggcg gagagtttat
     1921 tcaggttggt tcagatggag ggctcctgcc gcgatctgtt aaactgaact ctttcagcct
     1981 tgcgcctgct gaacgttatg atatcatcat tgacttcaca gcatatgaag gagaatcgat
     2041 cattttggca aacagcgcgg gctgcggcgg tgacgtcaat cctgaaacag atgcgaatat
     2101 catgcaattc agagtcacaa aaccattggc acaaaaagac gaaagcagaa agccggagta
     2161 cctcgcctca tacccttcgg tacagcatga gagaatacaa aacatcagaa cgttaaaact
     2221 ggcaggcacc caggacgaat acggcagacc cgtccttctg cttaataaca aacgctggca
     2281 cgatcccgtc acaggagcac caaaagtcgg cacaactgaa atatggtcca ttatcaaccc
     2341 gacacgcgga acacatccga tccacctgca tctagtctcc ttccgcgtat tagaccggcg
     2401 gccgtttgat atcgcccgtt atcaagaaag cggggaattg tcctataccg gtccggctgt
     2461 cccgccgccg ccaagtgaag agggctggaa agacaccatt caagcgcatg caggtgaagt
     2521 cctgagaatc gcggcgacat tcggtccgta cagcggacga tacgtatggc attgccatat
     2581 tctggagcat gaagactatg acatgatgag accgatggat ataactgatc gccataaata
     2641 a
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