BBa_K1093000
1
sfBFP
sfBFP
2013-07-21T11:00:00Z
2015-05-08T01:09:07Z
sfBFP is based on superfolder Green Fluorescent Protein (BBa_I746919), with Y66H mutation
Reference
[1]P??delacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology, 24(1), 79???88. doi:10.1038/nbt1172
Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence.
Excitation peak: 380 nm
Emission peak: 440 nm
false
false
_1403_
0
4695
9
It's complicated
false
Y66H mutation was introduced into BBa_I746919 via site-directed mutagenesis
false
Jakub Piątkowski
annotation2331035
1
sfBFP
range2331035
1
1
715
annotation2331033
1
start codon
range2331033
1
1
3
annotation2331034
1
stop codon
range2331034
1
715
717
BBa_K1093000_sequence
1
atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgactcatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z