BBa_K1093020 1 BBa_K1093020 HBA+NsfGFP (+B0034) 2013-09-20T11:00:00Z 2015-05-08T01:09:08Z The sequence was synthetized by GeneRay Human hemoglobin alpha chain fused at C-terminus with the N-terminal fragment of superfolder green fluorescent protein. Along with BBa_K1093021 and BBa_K1093031 (hemoglobin beta chain fused with two variants of C-terminal of sfGFP) it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to ihibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli. false false _1403_ 0 4695 9 Not in stock false HBA and sfGFP are fused with a flexible linker false Jakub Piątkowski annotation2357347 1 B0034 range2357347 1 2 13 annotation2357348 1 HBA-NsfGFP range2357348 1 20 1141 annotation2357350 1 stop range2357350 1 1139 1144 annotation2357351 1 B0010+B0012 range2357351 1 1154 1281 annotation2357349 1 start range2357349 1 20 22 BBa_K1093020_sequence 1 taaagaggagaaatactagatggtgctgagcccggcggataaaaccaacgtgaaagcggcgtggggcaaagtgggcgcgcatgcgggcgaatatggcgcggaagcgctggaacgcatgtttctgagctttccgaccaccaaaacctattttccgcattttgatctgagccatggcagcgcgcaggtgaaaggccatggcaaaaaagtggcggatgcgctgaccaacgcggtggcgcatgtggatgatatgccgaacgcgctgagcgcgctgagcgatctgcatgcgcataaactgcgcgtggatccggtgaactttaaactgctgagccattgcctgctggtgaccctggcggcgcatctgccggcggaatttaccccggcggtgcatgcgagcctggataaatttctggcgagcgtgagcaccgtgctgaccagcaaatatcgccgaccagcctgtaagattccaaatgacctgaagcagaaagttatgaatcacatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z