LOCUS       BBa_K1093023            3502 bp    DNA     linear       26-Dec-2024
DEFINITION  MinC/Lysis device-based kill switch
ACCESSION   BBa_K1093023
VERSION     BBa_K1093023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23100
     RBS             44..55
                     /label=B0034
     CDS             62..775
                     /label=cIts
     stem_loop       784..912
                     /label=B0010+B0012
     promoter        921..987
                     /label=pR
     RBS             996..1007
                     /label=B0034
     CDS             1014..1709
                     /label=MinC
     CDS             1734..2390
                     /label=t gene
     CDS             2418..2912
                     /label=e gene
     stem_loop       2924..3095
                     /label=B0010+B0012
     promoter        3061..3095
                     /label=J23100
     CDS             3118..3414
                     /label=antyholin
     stem_loop       3423..3502
                     /label=B0010
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgatacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      901 tctgcgttta tatactagag taacaccgtg cgtgttgact attttacctc tggcggtgat
      961 aatggttgct actagaaaga ggagaaatac tagagaaaga ggagaaatac tagatgtcaa
     1021 acacgccaat cgagcttaaa ggcagtagct tcactttatc tgtggttcat ctgcatgagg
     1081 cagaacctaa ggttatccat caggcgctgg aagacaaaat cgctcaggcc cccgcatttt
     1141 taaaacatgc ccccgttgta ctcaacgtca gtgcactgga agacccggta aactggtcag
     1201 cgatgcataa ggcggtttcg gcaaccggtt tgcgggttat tggcgtaagt ggctgcaaag
     1261 atgcgcaact taaagccgaa attgaaaaga tggggctgcc tatcctgacg gaaggaaagg
     1321 aaaaagcgcc acgtccagct cccacaccgc aggctccagc gcaaaataca acgccggtca
     1381 caaaaacgcg tttaatagat accccggtgc gttccggtca gcgtatttat gctccacaat
     1441 gtgatctgat tgttacaagc cacgttagcg ctggggccga attgattgcc gatgggaaca
     1501 ttcatgtcta tggcatgatg cgcggtcgtg cgctggcagg ggcaagtggt gaccgggaaa
     1561 cgcaaatatt ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt gaatactggc
     1621 tgagtgatca aatcccagca gaattttatg gcaaagcggc gcgactgcaa ttagtcgaaa
     1681 acgctttgac cgttcaaccg ttaaattgat actagagctt aaaaggaggg tctatggcag
     1741 cacctagaat atcattttcg ccctctgata ttctatttgg tgttctcgat cgcttgttca
     1801 aagataacgc taccgggaag gttcttgctt cccgggtagc tgtcgtaatt cttttgttta
     1861 taatggcgat tgtttggtat aggggagata gtttctttga gtactataag caatcaaagt
     1921 atgaaacata cagtgaaatt attgaaaagg aaagaactgc acgctttgaa tctgtcgccc
     1981 tggaacaact ccagatagtt catatatcat ctgaggcaga ctttagtgcg gtgtattctt
     2041 tccgccctaa aaacttaaac tattttgttg atattatagc atacgaagga aaattacctt
     2101 caacaataag tgaaaaatca cttggaggat atcctgttga taaaactatg gatgaatata
     2161 cagttcattt aaatggacgt cattattatt ccaactcaaa atttgctttt ttaccaacta
     2221 aaaagcctac tcccgaaata aactacatgt acagttgtcc atattttaat ttggataata
     2281 tctatgctgg aacgataacc atgtactggt atagaaatga tcatataagt aatgaccgcc
     2341 ttgaatcaat atgtgctcag gcggccagaa tattaggaag ggctaaataa ttatactaga
     2401 gatacttagg aggtattatg aatatatttg aaatgttacg tatagatgaa ggtcttagac
     2461 ttaaaatcta taaagacaca gaaggctatt acactattgg catcggtcat ttgcttacaa
     2521 aaagtccatc acttaatgct gctaaatctg aattagataa agctattggg cgtaattgca
     2581 atggtgtaat tacaaaagat gaggctgaaa aactctttaa tcaggatgtt gatgctgctg
     2641 ttcgcggaat cctgagaaat gctaaattaa aaccggttta tgattctctt gatgcggttc
     2701 gtcgctgtgc attgattaat atggttttcc aaatgggaga aaccggtgtg gcaggattta
     2761 ctaactcttt acgtatgctt caacaaaaac gctgggatga agcagcagtt aacttagcta
     2821 aaagtagatg gtataatcaa acacctaatc gcgcaaaacg agtcattaca acgtttagaa
     2881 ctggcacttg ggacgcgtat aaaaatctat aaagctacta gagccaggca tcaaataaaa
     2941 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     3001 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     3061 ttgacagcta gctcagtcct agggactatg ctagctacta gaggatagga ggcctttatg
     3121 gccttaaaag caacagcact ttttgccatg ctaggattgt catttgtttt atctccatcg
     3181 attgaagcga atgtcgatcc tcattttgat aaatttatgg aatctggtat taggcacgtt
     3241 tatatgcttt ttgaaaataa aagcgtagaa tcgtctgaac aattctatag ttttatgaga
     3301 acgacctata aaaatgaccc gtgctcttct gattttgaat gtatagagcg aggcgcggag
     3361 atggcacaat catacgctag aattatgaac attaaattgg agactgaatg aaattactag
     3421 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3481 ttgtttgtcg gtgaacgctc tc
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