LOCUS BBa_K1093024 5128 bp DNA linear 27-Dec-2024 DEFINITION MinC/Lysis device-based kill switch with delayed expression ACCESSION BBa_K1093024 VERSION BBa_K1093024.1 FEATURES Location/Qualifiers promoter 1..35 /label=J23100 RBS 44..55 /label=B0034 CDS 62..775 /label=cIts RBS 784..795 /label=B0034 CDS 802..1557 /label=LuxR stem_loop 1566..1694 /label=B0010+B0012 promoter 1703..1769 /label=pR RBS 1778..1789 /label=B0034 CDS 1796..2491 /label=MinC RBS 2500..2511 /label=B0034 CDS 2518..3132 /label=luxI stem_loop 3144..3272 /label=B0010+B0012 promoter 3281..3335 /label=pLux CDS 3360..4016 /label=t gene CDS 4044..4538 /label=e gene stem_loop 4550..4678 /label=B0010+B0012 promoter 4687..4721 /label=J23100 CDS 4744..5040 /label=antyholin stem_loop 5049..5128 /label=B0010 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgatacta 781 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 841 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 901 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 961 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 1021 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 1081 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 1141 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 1201 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 1261 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 1321 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 1381 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 1441 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 1501 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataatac 1561 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1681 tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc tctggcggtg 1741 ataatggttg ctactagaaa gaggagaaat actagagaaa gaggagaaat actagatgtc 1801 aaacacgcca atcgagctta aaggcagtag cttcacttta tctgtggttc atctgcatga 1861 ggcagaacct aaggttatcc atcaggcgct ggaagacaaa atcgctcagg cccccgcatt 1921 tttaaaacat gcccccgttg tactcaacgt cagtgcactg gaagacccgg taaactggtc 1981 agcgatgcat aaggcggttt cggcaaccgg tttgcgggtt attggcgtaa gtggctgcaa 2041 agatgcgcaa cttaaagccg aaattgaaaa gatggggctg cctatcctga cggaaggaaa 2101 ggaaaaagcg ccacgtccag ctcccacacc gcaggctcca gcgcaaaata caacgccggt 2161 cacaaaaacg cgtttaatag ataccccggt gcgttccggt cagcgtattt atgctccaca 2221 atgtgatctg attgttacaa gccacgttag cgctggggcc gaattgattg ccgatgggaa 2281 cattcatgtc tatggcatga tgcgcggtcg tgcgctggca ggggcaagtg gtgaccggga 2341 aacgcaaata ttttgtacga acctgatggc ggaactggtg tccatcgcag gtgaatactg 2401 gctgagtgat caaatcccag cagaatttta tggcaaagcg gcgcgactgc aattagtcga 2461 aaacgctttg accgttcaac cgttaaattg atactagaga aagaggagaa atactagatg 2521 actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt 2581 ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat 2641 aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat 2701 actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa 2761 agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa 2821 ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt 2881 acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat 2941 gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat 3001 cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg 3061 cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct 3121 ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3181 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3241 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt 3301 ttacgcaaga aaatggtttg ttatagtcga ataaatacta gagcttaaaa ggagggtcta 3361 tggcagcacc tagaatatca ttttcgccct ctgatattct atttggtgtt ctcgatcgct 3421 tgttcaaaga taacgctacc gggaaggttc ttgcttcccg ggtagctgtc gtaattcttt 3481 tgtttataat ggcgattgtt tggtataggg gagatagttt ctttgagtac tataagcaat 3541 caaagtatga aacatacagt gaaattattg aaaaggaaag aactgcacgc tttgaatctg 3601 tcgccctgga acaactccag atagttcata tatcatctga ggcagacttt agtgcggtgt 3661 attctttccg ccctaaaaac ttaaactatt ttgttgatat tatagcatac gaaggaaaat 3721 taccttcaac aataagtgaa aaatcacttg gaggatatcc tgttgataaa actatggatg 3781 aatatacagt tcatttaaat ggacgtcatt attattccaa ctcaaaattt gcttttttac 3841 caactaaaaa gcctactccc gaaataaact acatgtacag ttgtccatat tttaatttgg 3901 ataatatcta tgctggaacg ataaccatgt actggtatag aaatgatcat ataagtaatg 3961 accgccttga atcaatatgt gctcaggcgg ccagaatatt aggaagggct aaataattat 4021 actagagata cttaggaggt attatgaata tatttgaaat gttacgtata gatgaaggtc 4081 ttagacttaa aatctataaa gacacagaag gctattacac tattggcatc ggtcatttgc 4141 ttacaaaaag tccatcactt aatgctgcta aatctgaatt agataaagct attgggcgta 4201 attgcaatgg tgtaattaca aaagatgagg ctgaaaaact ctttaatcag gatgttgatg 4261 ctgctgttcg cggaatcctg agaaatgcta aattaaaacc ggtttatgat tctcttgatg 4321 cggttcgtcg ctgtgcattg attaatatgg ttttccaaat gggagaaacc ggtgtggcag 4381 gatttactaa ctctttacgt atgcttcaac aaaaacgctg ggatgaagca gcagttaact 4441 tagctaaaag tagatggtat aatcaaacac ctaatcgcgc aaaacgagtc attacaacgt 4501 ttagaactgg cacttgggac gcgtataaaa atctataaag ctactagagc caggcatcaa 4561 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4621 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 4681 ctagagttga cagctagctc agtcctaggg actatgctag ctactagagg ataggaggcc 4741 tttatggcct taaaagcaac agcacttttt gccatgctag gattgtcatt tgttttatct 4801 ccatcgattg aagcgaatgt cgatcctcat tttgataaat ttatggaatc tggtattagg 4861 cacgtttata tgctttttga aaataaaagc gtagaatcgt ctgaacaatt ctatagtttt 4921 atgagaacga cctataaaaa tgacccgtgc tcttctgatt ttgaatgtat agagcgaggc 4981 gcggagatgg cacaatcata cgctagaatt atgaacatta aattggagac tgaatgaaat 5041 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 5101 tatctgttgt ttgtcggtga acgctctc //