LOCUS       BBa_K109702             1144 bp    DNA     linear       22-Nov-2024
DEFINITION  LuxR generator regulated by Lac Promoter
ACCESSION   BBa_K109702
VERSION     BBa_K109702.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..228
                     /label=prefix
     misc_feature    400..400
                     /label=T
     misc_feature    718..718
                     /label=A
     CDS             227..976
                     /label=luxR
     misc_feature    227..982
                     /label=BBa_C0062
     misc_feature    983..1007
                     /label=Help:Barcodes
     CDS             227..982
                     /label=BBa_C0062
     stem_loop       1027..1070
                     /label=stem_loop
     misc_feature    1016..1095
                     /label=BBa_B0010
     terminator      1016..1095
                     /label=BBa_B0010
     stem_loop       1111..1130
                     /label=T7 TE
     misc_feature    1137..1137
                     /label=stop
     polyA_site      1131..1144
                     /label=polya
     misc_feature    1104..1144
                     /label=BBa_B0012
     terminator      1104..1144
                     /label=BBa_B0012
     misc_feature    209..1144
                     /label=BBa_I0462
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaacataaa
      241 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
      301 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
      361 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
      421 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
      481 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
      541 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
      601 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
      661 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
      721 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
      781 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
      841 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
      901 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
      961 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1141 tata
//