LOCUS       BBa_K1100011            1537 bp    DNA     linear       22-Nov-2024
DEFINITION  B0015 terminator efficiency estimation device (with Csy4 system)
ACCESSION   BBa_K1100011
VERSION     BBa_K1100011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0040
     misc_feature    1..19
                     /label=TetR 1
     promoter        20..25
                     /label=-35
     promoter        43..48
                     /label=-10
     RBS             79..89
                     /label=B0034
     stem_loop       62..77
                     /label=Insulator: Csy4 cleavage loci
     CDS             96..659
                     /label=Csy4
     misc_feature    1..659
                     /label=BBa_K1100009
     stem_loop       679..722
                     /label=stem_loop
     misc_feature    668..747
                     /label=BBa_B0010
     terminator      668..747
                     /label=BBa_B0010
     stem_loop       763..782
                     /label=T7 TE
     misc_feature    789..789
                     /label=stop
     polyA_site      783..796
                     /label=polya
     misc_feature    756..796
                     /label=BBa_B0012
     terminator      756..796
                     /label=BBa_B0012
     terminator      668..796
                     /label=BBa_B0015
     misc_feature    1..796
                     /label=BBa_K1100010
     stem_loop       811..826
                     /label=insulator: Csy4 cleavage loci
     RBS             826..838
                     /label=B0034
     CDS             845..1543
                     /label=sfGFP
     misc_feature    805..1537
                     /label=BBa_K1100006
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 actgccgtat aggcagcaaa gaggagaaat actagatgga ccactacctc gacattcgct
      121 tgcgaccgga cccggaattt cccccggcgc aactcatgag cgtgctcttc ggcaagctcc
      181 accaggccct ggtggcacag ggcggggaca ggatcggcgt gagcttcccc gacctcgacg
      241 aaagccgctc ccggctgggc gagcgcctgc gcattcatgc ctcggcggac gaccttcgtg
      301 ccctgctcgc ccggccctgg ctggaagggt tgcgggacca tctgcaattc ggagaaccgg
      361 cagtcgtgcc tcaccccaca ccgtaccgtc aggtcagtcg ggttcaggcg aaaagcaatc
      421 cggaacgcct gcggcggcgg ctcatgcgcc ggcacgatct gagtgaggag gaggctcgga
      481 aacgcattcc cgatacggtc gcgagagcct tggacctgcc cttcgtcacg ctacgcagcc
      541 agagcaccgg acagcacttc cgtctcttca tccgccacgg gccgttgcag gtgacggcag
      601 aggaaggagg attcacctgt tacgggttga gcaaaggagg tttcgttccc tggttctgat
      661 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      721 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      781 cctttctgcg tttatatact agagctgccg tataggcagc aaagaggaga aatactagat
      841 gagcaaaggc gaagaactgt ttaccggcgt ggtgccgatt ctggttgaac tggatggcga
      901 tgttaatggc cataagttta gcgtgcgcgg cgaaggcgaa ggcgatgcga ccaacggcaa
      961 actgaccctg aagtttattt gcaccaccgg caaactgccg gtgccgtggc cgaccctggt
     1021 gaccaccctg acctatggcg tgcagtgctt tagccgctat ccggatcaca tgaaacgcca
     1081 tgatttcttt aagtccgcga tgccggaagg ctatgttcag gaacgcacca ttagctttaa
     1141 agatgatggc acctataaga cccgcgcgga agtgaaattt gaaggcgata ccctggtgaa
     1201 ccgcattgaa ctgaaaggca ttgacttcaa agaagatggc aacattctgg gccataaact
     1261 ggagtacaat ttcaacagcc ataacgtgta tattaccgcg gacaaacaga agaacggcat
     1321 caaagcgaat ttcaaaatcc gccataatgt ggaagatggc agcgtgcagc tggcggatca
     1381 ctatcagcag aataccccga tcggcgatgg cccggtgctg ctgccggaca atcactacct
     1441 gagcacccag tccgtgctga gcaaagatcc gaatgagaaa cgcgatcaca tggttctgct
     1501 ggagtttgtg accgcggcgg gtatcactca tggctaa
//