LOCUS       BBa_K1100015             941 bp    DNA     linear       23-Dec-2024
DEFINITION  R0010 with insulator and sfGFP
ACCESSION   BBa_K1100015
VERSION     BBa_K1100015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     stem_loop       215..230
                     /label=insulator: Csy4 cleavage loci
     RBS             230..242
                     /label=B0034
     CDS             249..947
                     /label=sfGFP
     misc_feature    209..941
                     /label=BBa_K1100006
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagct gccgtatagg cagcaaagag gagaaatact
      241 agatgagcaa aggcgaagaa ctgtttaccg gcgtggtgcc gattctggtt gaactggatg
      301 gcgatgttaa tggccataag tttagcgtgc gcggcgaagg cgaaggcgat gcgaccaacg
      361 gcaaactgac cctgaagttt atttgcacca ccggcaaact gccggtgccg tggccgaccc
      421 tggtgaccac cctgacctat ggcgtgcagt gctttagccg ctatccggat cacatgaaac
      481 gccatgattt ctttaagtcc gcgatgccgg aaggctatgt tcaggaacgc accattagct
      541 ttaaagatga tggcacctat aagacccgcg cggaagtgaa atttgaaggc gataccctgg
      601 tgaaccgcat tgaactgaaa ggcattgact tcaaagaaga tggcaacatt ctgggccata
      661 aactggagta caatttcaac agccataacg tgtatattac cgcggacaaa cagaagaacg
      721 gcatcaaagc gaatttcaaa atccgccata atgtggaaga tggcagcgtg cagctggcgg
      781 atcactatca gcagaatacc ccgatcggcg atggcccggt gctgctgccg gacaatcact
      841 acctgagcac ccagtccgtg ctgagcaaag atccgaatga gaaacgcgat cacatggttc
      901 tgctggagtt tgtgaccgcg gcgggtatca ctcatggcta a
//