BBa_K1100015 1 BBa_K1100015 R0010 with insulator and sfGFP 2013-09-12T11:00:00Z 2015-05-08T01:09:08Z coming soon coming soon false false _1410_ 0 14489 9 It's complicated false coming soon false Chenxi He, Jiadong Ni component2354827 1 BBa_K1100006 component2354817 1 BBa_R0010 annotation2354827 1 BBa_K1100006 range2354827 1 209 941 annotation2354817 1 BBa_R0010 range2354817 1 1 200 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1100006 1 BBa_K1100006 optimized sfGFP with Csy4 insulator loci 2013-09-12T11:00:00Z 2015-05-08T01:09:08Z The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements. false false _1410_ 0 14489 9 Not in stock false Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated false Hao-tian Guo annotation2339687 1 B0034 range2339687 1 22 34 annotation2339478 1 insulator: Csy4 cleavage loci range2339478 1 7 22 annotation2339688 1 sfGFP range2339688 1 41 739 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1100015_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa BBa_K1100006_sequence 1 ctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z